Immunohistochemical evidence of cytokine networks during progression of human melanocytic lesions

Moretti, Silvia; Pinzi, Cinzia; Spallanzani, Adelina; Berti, Emilio; Chiarugi, Alessandra; Mazzoli, Sandra; Fabiani, Massimo; Vallecchi, C; Herlyn, Meenhard

doi:10.1002/(sici)1097-0215(19990420)84:2<160::aid-ijc12>3.3.co;2-i

Cited by 29 publications

(45 citation statements)

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“…TNF-a exerts a role in cytotoxic events 21 and may be involved in the disappearance of halo nevus cells. Interestingly, bFGF was not expressed in compound nevi, and TNF-a was detected in more than half of the lesions (significantly less than in halo nevi); this result appears in agreement with our previous data 12 and data of other authors. 22 A certain number of macrophages, only present in melanomas, expressed the same cytokines as melanoma cells.…”

Section: Table 3b Expression Of Cytokines On Melanocytic Cells In Masupporting

confidence: 93%

“…The cytokines involved in the regulation of fibrotic evolution (IL-6, TGF-b, TNF-a, bFGF, and PDGF) and of TAM (MPC-1 and GM-CSF) were expressed by melanoma cells in accordance with previous data, 12,14,18,19 irrespective of the presence of regression. In this and in another of our previous studies, 12 TGF-b was expressed in PM that were thicker than 1 mm; this finding does not completely match another report 20 with regard to TGF-b2 transcript expression in PM, where TGF-b2 was also found in few of Clark's level II invasive lesions.…”

Section: Table 3b Expression Of Cytokines On Melanocytic Cells In Masupporting

confidence: 89%

“…According to our data, the milieu of cytokines cannot completely explain the fibrotic evolution in RPM, but it is noteworthy that immunoreactivity of the antifibrogenic cytokine TNF-a was higher in halo nevi than in melanomas. This cytokine has already been described as weakly expressed in nevus and melanoma cells 12 and in the inflammatory infiltrate of halo nevus; 8 it might exert a role in nonfibrotic evolution in halo nevus.…”

Section: Table 3b Expression Of Cytokines On Melanocytic Cells In Mamentioning

confidence: 94%

“…Details of the method have been described elsewhere. 12 A two-step APAAP method, with Dako EnVision TM Labelle Polymer, Alkaline Phosphatase, was used when primary antibody was polyclonal rabbit (anti-MPC-1 and anti-PDGF-AA and anti-PDGF-BB) antibody. In this case, negative control sections were incubated with normal rabbit IgG.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…This difference would seem to be because of the process of malignant transformation and tumor progression that are associated with a higher expression of cytokines and growth factors, leading to a progressive growth independence of malignant cells. 12,13 Thus, the presence of fibrosis in RPM does not appear related to the fibrogenic cytokines alone, but it is possibly because of a complex network of cytokines and growth factors that are more dependent on the whole microenvironment of the tumor, possibly involving interactions between tumor and inflammatory infiltrate (especially macrophages?) and giving rise to signaling loops not clearly understood in depth.…”

Section: Table 3b Expression Of Cytokines On Melanocytic Cells In Mamentioning

confidence: 99%

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Fibrosis in regressing melanoma versus nonfibrosis in halo nevus upon melanocyte disappearance: Could it be related to a different cytokine microenvironment?

et al. 2007

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Fibrogenic cytokines were more frequently expressed in melanoma than in halo nevus, irrespective of regression. At ultrastructural level, melanocytes showed a more activated status in regressing melanoma than in halo nevus, in compliance with a milieu richer in cytokines. Although the cytokine microenvironment does not completely justify the fibrotic evolution in regressing PM, the higher TNF-alpha expression in halo nevus suggests a possible role in nonfibrotic evolution of this lesion.

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Section: Table 3b Expression Of Cytokines On Melanocytic Cells In Masupporting

confidence: 93%

Section: Table 3b Expression Of Cytokines On Melanocytic Cells In Masupporting

confidence: 89%

Section: Table 3b Expression Of Cytokines On Melanocytic Cells In Mamentioning

confidence: 94%

Section: Immunohistochemistrymentioning

confidence: 99%

Section: Table 3b Expression Of Cytokines On Melanocytic Cells In Mamentioning

confidence: 99%

See 3 more Smart Citations

Fibrosis in regressing melanoma versus nonfibrosis in halo nevus upon melanocyte disappearance: Could it be related to a different cytokine microenvironment?

et al. 2007

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High levels of extracellular ATP lead to chronic inflammatory response in melanoma patients

Mânica

Silva

Cardoso

et al. 2018

J of Cellular Biochemistry

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Skin cancer represents a serious public health problem and melanoma is considered the most significant due to its high metastasis capacity. Evasion mechanisms are the main characteristic of these tumor cells to escape of immune response. Extracellular nucleotides and nucleosides play an important role in inflammatory and immune responses. In this study, we analyzed the expression and activity of purinergic system enzymes in platelets and lymphocytes, ATP levels quantification, as well the level of pro and anti-inflammatory interleukins in the serum of 23 patients with surgical melanoma removal (CM group) and 23 control subjects (CT group). Results showed a decrease in ATP, ADP, and AMP hydrolysis and an increase in ATP levels quantification in CM group. The pro-inflammatory cytokines were elevated in CM group when compared to CT group. These results suggest an inflammatory process, even after surgical removal, due to elevated extracellular ATP levels. Besides, CM group displayed an increase in IL-10 levels and an increased in ADA activity in platelets and lymphocytes. Once adenosine and IL-10 are anti-inflammatory molecules, these results indicate a down-regulation of immune system front to malignant process. The alteration in nucleotide and nucleoside hydrolysis reinforces the purinergic systems role in this cancer. Therefore, even after surgical removal, the purinergic system can develop a chronic inflammatory micro-environment that can influence directly on relapse or metastasis.

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ROCK and JAK1 Signaling Cooperate to Control Actomyosin Contractility in Tumor Cells and Stroma

et al. 2011

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Proinflammatory cytokines are frequently observed in the tumor microenvironment, and chronic inflammation is involved in cancer initiation and progression. We show that cytokine signaling through the receptor subunit GP130-IL6ST and the kinase JAK1 generates actomyosin contractility through Rho-kinase dependent signaling. This pathway generates contractile force in stromal fibroblasts to remodel the extracellular matrix to create tracks for collective migration of squamous carcinoma cells and provides the high levels of actomyosin contractility required for migration of individual melanoma cells in the rounded, "amoeboid" mode. Thus, cytokine signaling can generate actomyosin contractility in both stroma and tumor cells. Strikingly, actomyosin contractility itself positively modulates activity of the transcription factor STAT3 downstream of JAK1, demonstrating positive feedback within the signaling network.

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Immunohistochemical evidence of cytokine networks during progression of human melanocytic lesions

Cited by 29 publications

References 0 publications

Fibrosis in regressing melanoma versus nonfibrosis in halo nevus upon melanocyte disappearance: Could it be related to a different cytokine microenvironment?

Fibrosis in regressing melanoma versus nonfibrosis in halo nevus upon melanocyte disappearance: Could it be related to a different cytokine microenvironment?

High levels of extracellular ATP lead to chronic inflammatory response in melanoma patients

ROCK and JAK1 Signaling Cooperate to Control Actomyosin Contractility in Tumor Cells and Stroma

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