Immunohistochemical Evaluation of E-Cadherin and α-Catenin Expression in Human Esophageal Cancer

Kadowaki, Takatoshi; Shiozaki, Hitoshi; Iihara, Keisuke; Inoue, Masatoshi; Tamura, Shinji; Oka, Hiroshi; Doki, Yuichiro; Matsui, Shigeo; Iwazawa, Takashi; Shimaya, K; Nagafuchi, Akira; Tsukita, Sachiko; Mori, Takesada

doi:10.1007/978-4-431-68252-3_31

Cited by 104 publications

(147 citation statements)

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“…This difference might be due to fractionated irradiation and acquisition of radioresistance. Some studies have shown that reduced E-cadherin expression was correlated with a high incidence of cancer metastasis and invasion (Doki et al, 1993;Kadowaki et al, 1994), and radiation enhanced the ability to form metastases (Sheldon and Fowler, 1976). Our results also suggest that the tumour cells that acquired radioresistance may have higher invasive ability and metastatic potential.…”

Section: Genes Related To Cell Adhesion and Motilitysupporting

confidence: 67%

Differential gene expression profiles of radioresistant oesophageal cancer cell lines established by continuous fractionated irradiation

et al. 2004

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Section: Genes Related To Cell Adhesion and Motilitysupporting

confidence: 67%

Differential gene expression profiles of radioresistant oesophageal cancer cell lines established by continuous fractionated irradiation

et al. 2004

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“…28 Recent studies have demonstrated that loss of E-cadherin immunoreactivity may correlate with a lack of differentiation and aggressive behavior of breast, head and neck, lung, gastric, esophageal, colorectal, prostatic and urothelial carcinomas. [5][6][7]15,[29][30][31][32][33][34][35] Published studies on urothelial carcinoma show that loss of membranous E-cadherin immunoreactivity is strongly correlated with high grade and advanced stage. 8,12,15,[36][37][38] Whether or not loss of E-cadherin is correlated with disease progression or has any prognostic significance has been debated by several investigators.…”

Section: Discussionmentioning

confidence: 99%

E-cadherin expression in plasmacytoid, signet ring cell and micropapillary variants of urothelial carcinoma: comparison with usual-type high-grade urothelial carcinoma

et al. 2011

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Loss of E-cadherin expression has been linked to the invasive phenotypes of a variety of neoplasms, including lobular breast cancer. The expression of E-cadherin in variants of urothelial carcinoma relative to usual-type urothelial carcinoma, maximum depth of invasion and angiolymphatic invasion has not been well characterized. A total of eight cases of micropapillary urothelial carcinoma, four cases of plasmacytoid urothelial carcinoma, two cases of urothelial carcinoma with signet ring cell differentiation and two cases of urothelial carcinoma with mixed plasmacytoid and signet ring cell differentiation, all obtained from cystectomy/cystoprostatectomy cases, were identified. In all nine cases of usual-type invasive and noninvasive high-grade urothelial carcinoma were also included in the study. Immunohistochemical staining of E-cadherin was performed in all cases. Pathological parameters including depth of invasion and presence of angiolymphatic invasion were documented. Maximum depth of invasion: In micropapillary urothelial carcinoma, extravesical extension was seen in three of eight cases; muscularis propria invasion in four of eight cases; and lamina propria invasion in one of eight cases. In plasmacytoid urothelial carcinoma, extravesical extension was observed in two of four cases, and muscularis propria invasion and lamina propria invasion in one of four cases each. In urothelial carcinoma with signet ring cell differentiation, extravesical extension and muscularis propria invasion was seen in one of two cases each. In urothelial carcinoma with mixed plasmacytoid and signet ring cell differentiation, muscularis propria invasion and lamina propria invasion was observed in one of two cases each. In usual-type high-grade urothelial carcinoma, extravesical extension was seen in six of nine cases and noninvasive in three of nine cases. In angiolymphatic invasion, micropapillary urothelial carcinoma was observed in eight of eight cases; plasmacytoid urothelial carcinoma in two of four cases; urothelial carcinoma with signet ring cell differentiation in one of two cases; and urothelial carcinoma with mixed plasmacytoid and signet ring cell differentiation in one of two cases. Usual-type high-grade urothelial carcinoma was seen in six of nine cases. E-cadherin expression: All eight cases of micropapillary urothelial carcinoma were positive for E-cadherin in the micropapillary component and adjacent usual-type urothelial carcinoma. The four cases of plasmacytoid urothelial carcinoma, two cases of urothelial carcinoma with signet ring cell differentiation and two cases of urothelial carcinoma with mixed plasmacytoid and signet ring cell differentiation were all negative for E-cadherin. All nine additional cases of usual-type high-grade urothelial carcinoma were diffusely positive for E-cadherin. E-cadherin is diffusely positive in usual-type urothelial carcinoma and micropapillary urothelial carcinoma, irrespective of pathological stage and angiolymphatic invasion. Loss of E-cadherin expression may be a marker of...

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“…detergent-soluble fraction (lanes Is and 2s) and insoluble fraction (lane li and 2i), (b) (Kadowaki et al. 1994;Matsui et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Effect of epidermal growth factor on cadherin-mediated adhesion in a human oesophageal cancer cell line

Shiozaki

Kadowaki²,

Doki³

et al. 1995

Br J Cancer

124

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SummanrEpidermal growth factor (EGF) mediates many pleiotrophic biological effects, one of which is alteration of cellular morphology. In the present study. we examine the possibility that this alteration in cell morphology is caused in part by the dysfunction of cadherin-mediated cell-cell adhesion using the human oesophageal cancer cell line TE-2R. which expresses E-cadherin and EGF receptor. In the presence of EGF. TE-2R changed its shape from round to fibroblastic and its colony formation from compact to sparse. Vanadate. a tyrosine phosphatase inhibitor, further potentiated the EGF response. whereas herbimycin A. a tvrosine kinase inhibitor, interfered with it. Moreover. EGF enabled the cells to invade in organotypic raft culture. These phenomena were accompanied not by decreased expression of the E-cadherin molecule but by a change in its localisation from the lateral adhesion site to the whole cell surface. Both a-and P-catenin.cadherin-binding proteins, were also expressed at the same level throughout these morphological changes.Finally. we examined tyrosine phosphorylation of E-cadherin and a-and P-catenin. and observed tyrosine phosphorylation of -catenin induced by EGF. These results suggest that EGF counteracts E-cadherinmediated junctional assembly through phosphorylation of P-catenin and modulates tumour cell behaviour to a more aggressive phenotype.

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Immunohistochemical Evaluation of E-Cadherin and α-Catenin Expression in Human Esophageal Cancer

Cited by 104 publications

References 3 publications

Differential gene expression profiles of radioresistant oesophageal cancer cell lines established by continuous fractionated irradiation

Differential gene expression profiles of radioresistant oesophageal cancer cell lines established by continuous fractionated irradiation

E-cadherin expression in plasmacytoid, signet ring cell and micropapillary variants of urothelial carcinoma: comparison with usual-type high-grade urothelial carcinoma

Effect of epidermal growth factor on cadherin-mediated adhesion in a human oesophageal cancer cell line

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