Immunohistochemical distribution of vanilloid receptor, calcitonin-gene related peptide and substance P in gastrointestinal mucosa of patients with different gastrointestinal disorders

Abstract

The immunohistochemical distribution of capsaicin/vanilloid (transient receptor potential vanilloid 1, TRPV1) receptors and neuropeptides (CGRP, SP) was studied in the gastrointestinal mucosal biopsies of patients with gastritis, erosions, ulcers, polyps, adenocarcinoma, chronic inflammatory bowel diseases, polyps without and with hyperplasia, dysplasia and adenocarcinoma in colon. The studies were carried out in 127 patients and 30 people with only functional dyspepsia (without any histological alteration). The results were: (1) the positivity of TRPV1 receptor and CGRP was detected, and weak participation of SP was detected in patients with different gastric diseases; (2) the presence of TRPV1, CGRP and SP could be detected in chronic inflammation of bowel disease; (3) SP could not detected in patients with colon polyps, dysplasia and adenocarcinoma; (4) the presence of TRPV1 and CGRP was proved in colon dysplasia and adenocarcinoma. We conclude that (1) the immunohistochemical distribution of TRPV1, CGRP and SP differs in gastrointestinal diseases of the upper and lower tract, and (2) the participation of TRPV1, CGRP and SP differs significantly in these different gastrointestinal diseases.

CXCL12-CXCR4 Promotes Proliferation and Invasion of Pancreatic Cancer Cells

“…However, more recently, this axis has been found to not only contribute to immunoregulation and mediatiation of the normal inflammatory response, but also play an important role in human immunological diseases, especially in mediating HIV infection (Pitcher et al, 2010;Patrussi and Baldari, 2011). Increasingly, studies have been devoted to uncovering the effects of CXCL12-CXCR4 on behavior of tumor cells, especially on proliferation, differentiation, directional migration, infiltration, and invasion of malignant cells (Horuk, 2001;Dömötör et al, 2005).…”

Approach to Role of Capsaicin - Sensitive Afferent Nerves in the Development and Healing in Patients with Chronic Gastritis

“…The possible role(s) of the capsaicin-sensitive afferent vagal nerve has been studied by our work-team since the l980s under physiological and different pathological conditions in animal experiments (Mózsik et al, 1997(Mózsik et al, , 2001(Mózsik et al, , 2004bAbdel Salam et al, 1999), healthy subjects (Mózsik et al, 2004b(Mózsik et al, , 2005a and in patients with different gastrointestinal disorders (Dömötör et al, 2005. The distribution of TRVP1, CGRP significantly increased in H. pylori positive chronic gastritis (in comparison with their distribution in gastric mucosa of healthy subjects), which data suggests that the TRPV1 and CGRP are involved in the development of human chronic gastritis (Tables 6 and 7).…”

“…Similar conclusions were obtained from the results of animal experiments and human observations, when we co-applied drugs acting on efferent and afferent vagal nerve fibres (Mózsik et al, 1997(Mózsik et al, , 2009) and received combined actions. Recently, different molecular-pharmacological observations were carried out (and calculated, based on the dose-responses curves of drugs) with capsaicin, atropine, pirenzepine, cimetidine, ranitidine, famotidine, nizatidine, omeprazole, esomeprazole, PGI 2, vitamin A, -carotene on the gastric basal acid output (BAO) in healthy human subjects (Mózsik et al, 2005), on changes of gastric transmucosal potential difference produced by ethanol (5 ml, 30 v/v% topically applied directly into the gastric mucosa by the way of endoscopic biopsy channel), on indomethacin-induced (3x25+25 mg orally) gastric microbleeding in healthy human subjects (Mózsik et al, 2007), and on gastric (basal and stimulated by betanechol, histamine and pentagastrin) acid secretion of pylorus-ligated rats or on gastric mucosal damage produced by different chemicals (Indomethacin, HCl, ethanol, NaOH, concentrated NaCl solution) in rats . The values of affinity (pD) and intrinsic activity ( ) curves of these compounds were calculated according to standard procedures employed in molecular-pharmacology (Csáky, 1969).…”

“…The possible role of afferent vagal nerve was studied in the last decades both in development of gastrointestinal mucosal damage and protection (Mózsik et al, 1997;2004a;Holzer, 1999;Abdel-Salam et al, 1999). Recently, the gastroprotective effect of capsaicin against chemical agents (ethanol, indomethacin) has been proven in human healthy subjects (Mózsik et al, 2004b(Mózsik et al, , 2005. The beneficial effect of capsaicin has also been shown in patients with functional gastrointestinal disorders (Bartolotti et al 2002;Bhat & Bielefeldt, 2006).…”

“…The immunodistribution of neuropeptides (SP, VIP, NPY, SOM, GAL, and TH) released from the sensory neurons and their neuroimmune function are known in H. pylori positive gastritis, but not have been examined in gastritis without H. pylori infection (Sipos et al, 2006). The presence of this receptor and released neurotransmitters could be studied in the development of human gastrointestinal disorders including gastritis, peptic ulcer, polyp without and with dysplasia, tumour and inflammatory bowel diseases by immunhistological method (Kihara et al, 2003;Zhang et al, 2005b;Dömötör et al, 2005;Mózsik et al, 2007). In our further research significant changes were observed in the immunhistological distribution of TRPV1, CGRP and SP in patients with chronic H. pylori positive gastritis and in histological healthy subjects but no change could be detected between the patients suffered from chronic gastritis without or with H. pylori infection .…”