Immunohistochemical differential distribution of S‐100α and S‐100β in the peripheral nervous system of the rat

Sugimura, Kimiya; Haimoto, Hajime; Nishimura, Hiroshi; Kato, Kanefusa; Takahashi, Akira

doi:10.1002/mus.880121109

Cited by 41 publications

(27 citation statements)

References 14 publications

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“…According to Vega et al (7), not all SGC are positive for vimentin; however, we found positive immunoreactivity in the entire population of SGC regardless of the type or size of the neuron. Observation of this reaction by confocal microscopy revealed that the expression of vimentin is more evident near the membrane, which is interesting because it is known that vimentin is a protein that participates in the communication between the cell surface and the nucleus (16). In view of these considerations, our results suggest that vimentin may exert its function through interaction with actin microfilaments, which are typically located under the cytoplasmic membrane.…”

supporting

confidence: 57%

Diversity among satellite glial cells in dorsal root ganglia of the rat

Nascimento

Santiago

Marques

et al. 2008

Braz J Med Biol Res

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Peripheral glial cells consist of satellite, enteric glial, and Schwann cells. In dorsal root ganglia, besides pseudo-unipolar neurons, myelinated and nonmyelinated fibers, macrophages, and fibroblasts, satellite cells also constitute the resident components. Information on satellite cells is not abundant; however, they appear to provide mechanical and metabolic support for neurons by forming an envelope surrounding their cell bodies. Although there is a heterogeneous population of neurons in the dorsal root ganglia, satellite cells have been described to be a homogeneous group of perineuronal cells. Our objective was to characterize the ultrastructure, immunohistochemistry, and histochemistry of the satellite cells of the dorsal root ganglia of 17 adult 3-4-month-old Wistar rats of both genders. Ultrastructurally, the nuclei of some satellite cells are heterochromatic, whereas others are euchromatic, which may result from different amounts of nuclear activity. We observed positive immunoreactivity for S-100 and vimentin in the cytoplasm of satellite cells. The intensity of S-100 protein varied according to the size of the enveloped neuron. We also noted that vimentin expression assumed a ring-like pattern and was preferentially located in the cytoplasm around the areas stained for S-100. In addition, we observed nitric oxide synthase-positive small-sized neurons and negative large-sized neurons equal to that described in the literature. Satellite cells were also positive for NADPH-diaphorase, particularly those associated with small-sized neurons. We conclude that all satellite cells are not identical as previously thought because they have different patterns of glial marker expression and these differences may be correlated with the size and function of the neuron they envelope.

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supporting

confidence: 57%

Diversity among satellite glial cells in dorsal root ganglia of the rat

Nascimento

Santiago

Marques

et al. 2008

Braz J Med Biol Res

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“…The present results suggest that S100 protein of sensory corpuscles consists of S100␣ and S100␤ proteins, as it was demonstrated for glial cells of the peripheral nervous system (Sugimura et al, 1989). The homo-or heterodimeric combination of S100␣ and S100␤ proteins predominate in the mammalian peripheral nervous system (for references see Schä fer and Heizmann, 1996).…”

Section: Discussionmentioning

confidence: 67%

“…Thus, the central axons of cutaneous sensory corpuscles, at least in man, lack S100 proteins. Consequently, it must be assumed that sensory neurons containing S100 (Vega et al, 1989;Ichikawa et al, 1997), S100␣ (Isobe et al, 1984;Sugimura et al, 1989;Vega et al, 1989Vega et al, , 1991, and S100␤ (Sugimura et al, 1989;Vega et al, 1991;Yang et al, 1995) proteins do not project to the skin, do not exist in adult men, and do not form cutaneous sensory corpuscles (for references see Vega et al, 1996), or they lack this antigen just in that localization. Recently we observed that neuronal S100 proteins in dorsal root ganglion sensory neurons occur only in the rat and not in other mammals, including man (Albuerne et al, unpublished data).…”

Section: Discussionmentioning

confidence: 98%

“…They have been found in satellite glial cells of the peripheral ganglia as well as in Schwann and Schwann-related cells. Moreover, S100 proteins (Sugimura et al, 1989;Vega et al, 1989Vega et al, , 1991 and S100␤ protein (Isobe et al, 1984;Sugimura et al, 1989;Vega et al, 1989Vega et al, , 1991Yang et al, 1995;Ichikawa et al, 1997) were observed in peripheral neurons. Among Schwann-related cells displaying S100 protein immunoreactivity (IR) are the lamellar cells of Meissner corpuscles and the inner-core cells of Pacinian corpuscles (Iwanaga et al, 1982; for references see Vega et al, 1996).…”

mentioning

confidence: 94%

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S100α and S100β proteins in human cutaneous sensory corpuscles: Effects of nerve and spinal cord injury

et al. 1998

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S100 protein in the vertebrate peripheral nervous system consists of homo- or heterodimers of S100alpha and S100beta proteins, the first predominating in neurons and the second in glial cells. Recently, however, occurrence of S100beta protein in neurons has been reported. The expression of S100 protein by Schwann cells, as well as their derivatives in sensory corpuscles, depends on the sensory axon (i.e., the Schwann cell-axon contact). The present study analyzed the distribution of S100alpha and S100beta proteins in human cutaneous sensory corpuscles and the effects of peripheral or central sensory axon severance in the expression of these proteins. Simple or double immunohistochemistry was carried out using a panel of antibodies against S100alpha, S100beta or S100alpha+beta proteins, and the sections were examined by light or laser confocal scanning microscopy. Skin samples were obtained from normal subjects and patients with spinal cord injury, nerve entrapment, and nerve sections plus graft. The lamellar cells of Meissner corpuscles as well as the inner-core lamellae of the Pacinian corpuscles displayed strong immunoreactivity (IR) for all antigens examined, the most intense labeling being obtained for S100beta protein. The pattern of immunostaining was unchanged after spinal cord injury, whereas the number of stained corpuscles as well as the intensity of IR for each antigen decreased in cutaneous sensory corpuscles after nerve injury, both entrapment and section plus graft. No evidence was found of axonal labeling. The present results provide evidence that Schwann-related cells in human cutaneous sensory corpuscles contain both S100alpha and S100beta and that the expression of these proteins is dependent on the functional and structural integrity of sensory fibers.

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“…No Sistema Nervoso Periférico SNP foi encontrada S100β em células de Schwann, sugerindo envolvimento na produção de mielina, e manutenção da meninge pia mater. (Sugimura et al, 1989;Mata et al, 1990).…”

Section: Discussionunclassified

Estudo da distribuição da proteína S100b em encéfalo de ratos.

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Immunohistochemical differential distribution of S‐100α and S‐100β in the peripheral nervous system of the rat

Cited by 41 publications

References 14 publications

Diversity among satellite glial cells in dorsal root ganglia of the rat

Diversity among satellite glial cells in dorsal root ganglia of the rat

S100α and S100β proteins in human cutaneous sensory corpuscles: Effects of nerve and spinal cord injury

Estudo da distribuição da proteína S100b em encéfalo de ratos.

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