Immunohistochemical diagnosis of human infectious diseases: a review

Hama, Hamadou Oumarou; Aboudharam, Gérard; Barbieri, Rémi; Lépidi, Hubert; Drancourt, Michel

doi:10.1186/s13000-022-01197-5

Cited by 13 publications

(6 citation statements)

References 238 publications

(186 reference statements)

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“…as a causative pathogen that may be increasingly found as a cause of infective endocarditis. It should be highlighted that infectious disease specialists should keep in mind the likelihood of borrelial etiology of endocarditis in endemic areas [ 102 , 103 ] ( Figure 4 ).…”

Section: Microbiologymentioning

confidence: 99%

Infective Endocarditis in High-Income Countries

et al. 2022

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Infective endocarditis remains an illness that carries a significant burden to healthcare resources. In recent times, there has been a shift from Streptococcus sp. to Staphylococcus sp. as the primary organism of interest. This has significant consequences, given the virulence of Staphylococcus and its propensity to form a biofilm, rendering non-surgical therapy ineffective. In addition, antibiotic resistance has affected treatment of this organism. The cohorts at most risk for Staphylococcal endocarditis are elderly patients with multiple comorbidities. The innovation of transcatheter technologies alongside other cardiac interventions such as implantable devices has contributed to the increased risk attributable to this cohort. We examined the pathophysiology of infective endocarditis carefully. Inter alia, the determinants of Staphylococcus aureus virulence, interaction with host immunity, as well as the discovery and emergence of a potential vaccine, were investigated. Furthermore, the potential role of prophylactic antibiotics during dental procedures was also evaluated. As rates of transcatheter device implantation increase, endocarditis is expected to increase, especially in this high-risk group. A high level of suspicion is needed alongside early initiation of therapy and referral to the heart team to improve outcomes.

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Section: Microbiologymentioning

confidence: 99%

Infective Endocarditis in High-Income Countries

et al. 2022

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“…Trachea, lung, and kidney tissues were collected and fixed in 10% neutral buffered formalin for further histopathological and immunohistochemical analysis, respectively. The immunohistochemical staining was conducted following previously reported protocols [ 20 ], using a monoclonal antibody against IBV N as the primary antibody. Meanwhile, total RNAs were extracted from the trachea, lung, and kidney tissues harvested at 7 days post-infection, and the viral RNA copy numbers were quantified by RT-qPCR.…”

Section: Methodsmentioning

confidence: 99%

Prevalence, Genotype Diversity, and Distinct Pathogenicity of 205 Gammacoronavirus Infectious Bronchitis Virus Isolates in China during 2019–2023

Xiong,

Xie,

et al. 2024

Viruses

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Gammacoronavirus infectious bronchitis virus (IBV) causes a highly contagious disease in chickens and seriously endangers the poultry industry. The emergence and co-circulation of diverse IBV serotypes and genotypes with distinct pathogenicity worldwide pose a serious challenge to the development of effective intervention measures. In this study, we report the epidemic trends of IBV in China from 2019 to 2023 and a comparative analysis on the antigenic characteristics and pathogenicity of isolates among major prevalent lineages. Phylogenetic and recombination analyses based on the nucleotide sequences of the spike (S) 1 gene clustered a total of 205 isolates into twelve distinct lineages, with GI-19 as a predominant lineage (61.77 ± 4.56%) exhibiting an overall increasing trend over the past five years, and demonstrated that a majority of the variants were derived from gene recombination events. Further characterization of the growth and pathogenic properties of six representative isolates from different lineages classified four out of the six isolates as nephropathogenic types with mortality rates in one-day-old SPF chickens varying from 20–60%, one as a respiratory type with weak virulence, and one as a naturally occurring avirulent strain. Taken together, our findings illuminate the epidemic trends, prevalence, recombination, and pathogenicity of current IBV strains in China, providing key information for further strengthening the surveillance and pathogenicity studies of IBV.

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“…Positive control and negative control are used to confirm the validity of individual tests [22]. Examples: Adenovirus [23], Rotavirus, and Norovirus can be diagnosed using this method (Figure 5) [24]. Trends of Diagnostic Methods for Human Viral Diseases DOI: http://dx.doi.org/10.5772/intechopen.113239…”

Section: Immunochromatographymentioning

confidence: 99%

Trends of Diagnostic Methods for Human Viral Diseases

Borkakoty,

Jakharia,

Singh

et al. 2024

Infectious Diseases

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The global health field is significantly affected by viral infections, and sero-diagnosis is crucial in diagnostic virology. Various laboratory techniques such as nucleic acid detection, viral culture, and antigen detection are essential for diagnosing viral infections. Advances in science have led to the development of new immunologic and molecular techniques, enabling rapid and simplified diagnosis of different viruses. Timely and accurate identification of viral infections is vital for effective outbreak management. Immunological techniques, detecting viral antigens or antibodies, are widely used in diagnostic and epidemiological research, aiding in epidemic identification, appropriate diagnostic tests, vaccination programs, and detecting common and emerging viruses. However, traditional viral identification methods demand extensive technical expertise, time, and financial resources. Consequently, scientists worldwide are dedicated to developing precise diagnostic methods for viral diseases. Various innovative approaches are being explored, aiming to create more accessible, time-efficient, and cost-effective viral disease diagnosis methods, thereby benefiting low-income countries.

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Immunohistochemical diagnosis of human infectious diseases: a review

Cited by 13 publications

References 238 publications

Infective Endocarditis in High-Income Countries

Infective Endocarditis in High-Income Countries

Prevalence, Genotype Diversity, and Distinct Pathogenicity of 205 Gammacoronavirus Infectious Bronchitis Virus Isolates in China during 2019–2023

Trends of Diagnostic Methods for Human Viral Diseases

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