Immunohistochemical determination of the P15^INK4b protein expression in cutaneous squamous cell carcinoma

Abstract

The tumor suppressor gene p15(INK4b) is a cyclin-dependent kinase inhibitor, in which its inactivation has been determined in primary tumors and in several tumor-derived cell lines. The precise role of p15(INK4b) protein expression in cutaneous squamous cell carcinoma (SCC) is currently not known. In a previous study, we have shown the frequent occurrence of allelic imbalance/loss of heterozygosity in cutaneous SCC using two microsatellite markers flanking the p15(INK4b) gene. This study is a continuation of our previous study and aims to determine the possible role of p15(INK4b) protein expression in the genesis of cutaneous SCC. P15(INK4b) protein expression was determined using immunohistochemical approach in 107 cases of cutaneous SCC tissue arrays and 19 cases of normal human skin tissues. The expression of p15(INK4b) was significantly reduced in the cutaneous SCC cases as compared with normal human skin (p = 0.017 and p < 0.05). However, there were no significant relationship between clinicopathologic variables of the patients (age, sex and tumor grade) and p15(INK4b) protein expression. The absence of p15(INK4b) expression in the majority of tissue microarray cores of cutaneous SCC indicated that p15(INK4b) could possibly be involved in the pathogenesis of cutaneous SCC.

Loss of p15INK4bExpression in Colorectal Cancer is Linked to Ethnic Origin

“…Consistent with our data, no correlation was observed between p15 INK4b and pathological or survival data apart from tendency to affect male gender and distal location within the stomach (Sakellariou et al, 2008). In cutaneous squamous cell carcinoma, p15 INK4b protein expression was absent in relationship between clinicopathologic variables of the patients (age, sex and tumor grade) and p15 INK4b protein expression (Moad et al, 2009 Wael Mohamed Abdel Rahman et al invasiveness. However, they could not establish p15 INK4b as independent prognostic markers (Holm et al, 2013).…”

Lack of Increased P15INK4BProtein Expression in Basal Cell Carcinomas

“…In previous study, we have shown the absence of p15 INK4b expression in the majority of tissue microarray cores of cutaneous SCC and this indicated that p15 INK4b could possibly be involved in the pathogenesis of cutaneous SCC (Moad et al, 2009). Alteration of the p15 INK4b gene has been reported in several tumour-derived cell lines and primary tumor, but the role played by this gene in the pathogenesis of BCC is not fully elucidated.…”

“…We have shown previously the absence of the expression of p15 INK4b protein in the majority of tissue microarray cores of cutaneous squamous cell carcinoma (Moad et al, 2009 (Kamb, 1998). Loss of p15 INK4b protein function is known to initiate continual activation of the CDK2-cyclin E complex, which is necessary for entry into the S phase of the cell cycle, resulting in an avoidance of cell cycle arrest and promotion of the neoplastic process (Hartwell and Kastan, 1994).…”

Systematical Analysis of Cutaneous Squamous Cell Carcinoma Network of microRNAs, Transcription Factors, and Target and Host Genes

“…Analysis of IRF6 expression in a large series of SCCs showed a strong down-regulation that correlated with tumor invasive and differentiation status (Botti et al, 2011). RPL15 and LGTN were down-regulated and the expression of p15(INK4b) was significantly reduced (Dang et al, 2006;Moad et al, 2009). The expression of KGF receptor (KGFR) mRNA was lower in cutaneous SCCs than in normal skin samples (Toriseva et al, 2012).…”