Immunohistochemical determination of calbindin-d28k in the kidney of postnatal rats

Timurkaan, Sema; Tarakçi, Berrin Gençer

doi:10.17221/5713-vetmed

Cited by 5 publications

(4 citation statements)

References 26 publications

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“…Isolated cells represented a mixture (approximately 50%:50%) of cytokeratin-positive epithelial cells and vimentin-positive non-epithelial cells. An in-depth analysis further showed that cells also expressed the renal collecting duct cell marker aquaporin-2 [ 27 ], the distal tubulus cell marker calbindin D28K [ 28 ] and the proximal tubulus cell marker alkaline phosphatase [ 29 ]. Hence, it was concluded that the isolated primary feline kidney cells express the terminal differentiation markers also present in naïve tissues and are therefore suitable for in vitro infection experiments.…”

Section: Resultsmentioning

confidence: 99%

A New Genotype of Feline Morbillivirus Infects Primary Cells of the Lung, Kidney, Brain and Peripheral Blood

Sieg

Busch

Eschke

et al. 2019

Viruses

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Paramyxoviruses comprise a large number of diverse viruses which in part give rise to severe diseases in affected hosts. A new genotype of feline morbillivirus, tentatively named feline morbillivirus genotype 2 (FeMV-GT2), was isolated from urine of cats with urinary tract diseases. Whole genome sequencing showed about 78% nucleotide homology to known feline morbilliviruses. The virus was isolated in permanent cell lines of feline and simian origin. To investigate the cell tropism of FeMV-GT2 feline primary epithelial cells from the kidney, the urinary bladder and the lung, peripheral blood mononuclear cells (PBMC), as well as organotypic brain slice cultures were used for infection experiments. We demonstrate that FeMV-GT2 is able to infect renal and pulmonary epithelial cells, primary cells from the cerebrum and cerebellum, as well as immune cells in the blood, especially CD4+ T cells, CD20+ B cells and monocytes. The cats used for virus isolation shed FeMV-GT2 continuously for several months despite the presence of neutralizing antibodies in the blood. Our results point towards the necessity of increased awareness for this virus when clinical signs of the aforementioned organs are encountered in cats which cannot be explained by other etiologies.

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Section: Resultsmentioning

confidence: 99%

A New Genotype of Feline Morbillivirus Infects Primary Cells of the Lung, Kidney, Brain and Peripheral Blood

Sieg

Busch

Eschke

et al. 2019

Viruses

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“…In the kidney, it is localized in the distal convoluted and collecting tubules. To date, the functional role of calbindin-D28k remains unclear (Timurkaan & Tarakci, 2004); however, its role as a carrier and buffer protein that facilitates the transcellular transport of Ca 2+ and prevents the increase in its concentration during transportation (Geng et al, 2015) was suggested. In this study, calbindin was localized in the young and adult camels' kidneys, particularly within the cells in the distal tubules, the main site for selective calcium reabsorption.…”

Section: Discussionmentioning

confidence: 99%

Aging-Related Functional and Structural Changes in Renal Tissues: Lesson from a Camel Model

et al. 2021

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“…For example, immunohistochemical staining for Tamm-Horsfall protein will label thick ascending limbs in all species, but this immunostain also labels distal convoluted tubule in rats and mice (Vekaria et al 2006). Immunohistochemical stains directed against calbindin-D28 K specifically label distal convoluted tubules in rodents (Timurkaan and Tarakci 2004). Alpha-GST immunostains label proximal tubules including S1 and S2 segments of the proximal convoluted tubules as well as S3 straight segments.…”

Section: Morphologymentioning

confidence: 99%

Proliferative and Nonproliferative Lesions of the Rat and Mouse Urinary System

et al. 2012

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The INHAND Project (International Harmonization of Nomenclature and Diagnostic Criteria for Lesions in Rats and Mice) is a joint initiative of the Societies of Toxicologic Pathology from Europe (ESTP), Great Britain (BSTP), Japan (JSTP), and North America (STP) to develop an internationally accepted nomenclature for proliferative and nonproliferative lesions in laboratory animals. The purpose of this publication is to provide a standardized nomenclature for classifying lesions observed in the urinary tract of rats and mice. The standardized nomenclature of urinary tract lesions presented in this document is also available electronically on the Internet (http://www.goreni.org/). Sources of material included histopathology databases from government, academia, and industrial laboratories throughout the world. Content includes spontaneous developmental and aging lesions as well as those induced by exposure to test materials. A widely accepted and utilized international harmonization of nomenclature for urinary tract lesions in laboratory animals will decrease confusion among regulatory and scientific research organizations in different countries and provide a common language to increase and enrich international exchanges of information among toxicologists and pathologists.

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Immunohistochemical determination of calbindin-d28k in the kidney of postnatal rats

Cited by 5 publications

References 26 publications

A New Genotype of Feline Morbillivirus Infects Primary Cells of the Lung, Kidney, Brain and Peripheral Blood

A New Genotype of Feline Morbillivirus Infects Primary Cells of the Lung, Kidney, Brain and Peripheral Blood

Aging-Related Functional and Structural Changes in Renal Tissues: Lesson from a Camel Model

Proliferative and Nonproliferative Lesions of the Rat and Mouse Urinary System

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