Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection

Canal, A.M.; Pezzone, Natalia; Cataldi, Angel; Zumárraga, Martín José; Larzábal, Mariano; Garbaccio, Sergio; Domı́nguez, Lucas; Aranaz, Alicia; Rodríguez‐Bertos, Antonio

doi:10.1016/j.rvsc.2016.10.006

Cited by 15 publications

(26 citation statements)

References 30 publications

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“…Similarly, neutrophil numbers in the granuloma are associated with increased M. bovis proliferation (33). Another study shows that as the stage of granuloma increases, macrophages and epithelioid cells mediate an increase in expression of cytokines (35). Still, little is known about the local immune response of CD3+ T cells, CD68+ macrophages, IFN-, TNF-α and iNOS in cattle naturally infected with M. bovis.…”

Section: Downloaded Frommentioning

confidence: 99%

Cellular and Cytokine Responses in the Granulomas of Asymptomatic Cattle Naturally Infected with Mycobacterium bovis in Ethiopia

et al. 2020

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Cells (CD3+ T cell and CD68+ macrophages), cytokines (IFN-γ+ and TNF-α+) and effector molecule (iNOS+) responses were evaluated in the lymph nodes and tissue of cattle naturally infected with Mycobacterium bovis. Detailed post mortem and immunohistochemical examinations of lesions were performed on 16 cows positive for single intradermal cervical comparative tuberculin (SICCT) test which were identified from dairy farms located around the Addis Ababa City. The severity of the gross lesion was significantly higher (p=0.003) in M. bovis culture positive (n=12) cows than in culture negative (n=4). Immunohistochemical techniques showed that in culture positive cows, the mean immunolabeling fraction of CD3+ T cells decreased as the stage of granuloma increased from stage I to stage IV (p<0.001). In contrast, the immunolabelling fraction of CD68+ macrophages, IFN-γ+, TNF-α+ and iNOS+ increased from stage I to stage IV (p< 0.001). In culture negative cows, early stages showed a significantly higher fraction of CD68+ macrophages (p=0.03) and iNOS+ (p=0.007) when compared to culture positive cows. Similarly, at advanced granuloma stages, culture negative cows demonstrated significantly higher mean proportions of CD3+ T cells (p< 0.001) compared to culture positive cows. Thus, this study demonstrates that following natural infection of cows with M. bovis, as the stage of granuloma increases from stage I to stage IV, the immunolabelling fraction of CD3+ cells decreases while the immunolabeling fraction of CD68+ macrophages, IFN-γ+, TNF-α+ and iNOS+ increases.

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Section: Downloaded Frommentioning

confidence: 99%

Cellular and Cytokine Responses in the Granulomas of Asymptomatic Cattle Naturally Infected with Mycobacterium bovis in Ethiopia

et al. 2020

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“…The degree of peripheral fibrosis is variable, and hosts that are more resistant to the disease tend to develop more exuberant fibrosis followed by central necrosis and mineralization, a phenomenon already described for cattle and cervids (Canal et al, 2017).…”

Section: Drivers Of Animal Tb Heterogeneitymentioning

confidence: 83%

“…The same work assessed the expression levels of another cytokine associated with granuloma development and revealed small differences in TNF‐α expression along the evolution process (Garcia‐Jimenez et al, 2012). Differential cytokine [IFN‐γ, TNF‐α, IL‐1β, IL‐10 and transforming growth factor beta (TGF‐β)] expression between the initial and late stages of granuloma development was also studied in naturally infected cattle using immunohistochemistry (Canal et al, 2017). Globally, cytokines’ expression in late stages (stages III and IV) was significant, predominantly IFN‐γ, once more associated with disease severity (Canal et al, 2017).…”

Section: Drivers Of Animal Tb Heterogeneitymentioning

confidence: 99%

“…Differential cytokine [IFN‐γ, TNF‐α, IL‐1β, IL‐10 and transforming growth factor beta (TGF‐β)] expression between the initial and late stages of granuloma development was also studied in naturally infected cattle using immunohistochemistry (Canal et al, 2017). Globally, cytokines’ expression in late stages (stages III and IV) was significant, predominantly IFN‐γ, once more associated with disease severity (Canal et al, 2017). Moreover, higher expression of IFN‐γ and TGF‐β was observed in lymphocytes, macrophages and necrotic areas of late‐stage granulomas, whereas IL‐10 immunoreactivity was absent from necrotic areas (Canal et al, 2017).…”

Section: Drivers Of Animal Tb Heterogeneitymentioning

confidence: 99%

“…Globally, cytokines’ expression in late stages (stages III and IV) was significant, predominantly IFN‐γ, once more associated with disease severity (Canal et al, 2017). Moreover, higher expression of IFN‐γ and TGF‐β was observed in lymphocytes, macrophages and necrotic areas of late‐stage granulomas, whereas IL‐10 immunoreactivity was absent from necrotic areas (Canal et al, 2017). TGF‐β is associated with fibrosis processes and so higher levels of this cytokine could indicate the establishment of the chronic process (Canal et al, 2017).…”

Section: Drivers Of Animal Tb Heterogeneitymentioning

confidence: 99%

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Animal tuberculosis: impact of disease heterogeneity in transmission, diagnosis, and control

Pereira

Reis

Ramos

et al. 2020

Transbound Emerg Dis

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Animal tuberculosis (TB) in terrestrial mammals is mainly caused by Mycobacterium bovis. This pathogen is adapted to a wide range of host species, representing a threat to livestock, wildlife and human health. Disease heterogeneity is a hallmark of multi‐host TB and a challenge for control. Drivers of animal TB heterogeneity are very diverse and may act at the level of the causative agent, the host species, the interface between mycobacteria and the host, community of hosts, the environment and even policy behind control programmes. In this paper, we examine the drivers that seem to contribute to this phenomenon. We begin by reviewing evidence accumulated to date supporting the consensus that a complex range of genetic, biological and socio‐environmental factors contribute to the establishment and maintenance of animal TB, setting the grounds for heterogeneity. We then highlight the complex interplay between individual, species‐specific and community protective factors with risk/maintenance variables that include animal movements and densities, co‐infection and super‐shedders. We finally consider how current interventions should seek to consider and explore heterogeneity in order to tackle potential limitations for diagnosis and control programmes, simultaneously increasing their efficacy.

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Extracellular matrix proteins (fibronectin, collagen III, and collagen I) immunoexpression in goat tuberculous granulomas (Mycobacterium caprae)

et al. 2022

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The lesion resulting from the interaction between Mycobacterium and the host immune response is the tuberculous granuloma. Tuberculous granulomas, except in incipient stages, are partially or totally encapsulated by connective tissue. The aim of this study was to assess the immunoexpression of the extracellular matrix proteins fibronectin, collagen III, and collagen I in granulomas caused by Mycobacterium caprae in goats (Capra aegagrus hircus) to understand capsule development at different granuloma stages. For this purpose, a retrospective study of 56 samples of tuberculous granulomas in lung (n = 30) and mediastinal lymph node (n = 26) from 17 goats naturally infected with M. caprae in stages I (n = 15), II (n = 14) and III (n = 27) was carried out. Fibronectin immunoreaction was extracellular, fibrillar-reticular in the center of stage I, II and III granulomas and peripheral in stages II and III granulomas. Collagen III immunoexpression was extracellular and fibrillar in the center of stages I, II and III tuberculous granulomas in lung and mediastinal lymph node, and progressive expression was observed in the periphery of stages II and III granulomas. Finally, collagen I immunoexpression was extracellular and fibrillar, showing a progressive loss of central expression and an increase in peripheral expression in stage III granulomas compared to stage I granulomas. Immunoexpression of these extracellular matrix proteins could help understand fibrogenesis and dating in tuberculous granuloma in both animal models and humans.

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Immunohistochemical detection of pro-inflammatory and anti-inflammatory cytokines in granulomas in cattle with natural Mycobacterium bovis infection

Cited by 15 publications

References 30 publications

Cellular and Cytokine Responses in the Granulomas of Asymptomatic Cattle Naturally Infected with Mycobacterium bovis in Ethiopia

Cellular and Cytokine Responses in the Granulomas of Asymptomatic Cattle Naturally Infected with Mycobacterium bovis in Ethiopia

Animal tuberculosis: impact of disease heterogeneity in transmission, diagnosis, and control

Extracellular matrix proteins (fibronectin, collagen III, and collagen I) immunoexpression in goat tuberculous granulomas (Mycobacterium caprae)

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