Immunohistochemical Detection of Oxidative Stress Biomarkers, Dityrosine and N&sup.EPSILON.;-(hexanoyl)lysine, and C-Reactive Protein in Rabbit Atherosclerotic Lesions

Abstract

Several lines of evidence have demonstrated that C-reactive protein (CRP) is associated with oxidative stress; however, the precise co-localization between CRP and oxidative stress markers in atherosclerotic lesions is not fully established. In this study, we focused on two oxidative stress markers, dityrosine (DY) and N -(hexanoyl)lysine (HEL), which had not previously been investigated in relation to CRP in atherosclerotic lesions. Aim: We investigated the production and localization of DY, HEL, and CRP in early-stage and moderately progressed fatty lesions of cholesterol-fed rabbits by immunohistochemistry using specific monoclonal antibodies to examine the co-localization between CRP and oxidative stress in atherosclerotic lesions. Methods: Rabbit atherosclerotic specimens were obtained from New Zealand White rabbits fed a diet containing 1.0% cholesterol for 12 weeks. All specimens were fixed in formalin for histological examinations.

Relations between Serum Reactive Oxygen Metabolites (ROMs) and Various Inflammatory and Metabolic Parameters in a Japanese Population

“…As for the mechanisms, Fukuchi, et al 32) reported the immunohistochemical detection of oxidative stress biomarkers, dityrosine and N -(hexanoyl)lysine, and C-reactive protein in rabbit atherosclerotic lesions.…”

High-Sensitivity CRP Reflects Insulin Resistance in Smokers

“…CRP is bound to the membranes of damaged vascular cells where it activates complement proteins or enhances the production of thrombogenic agents. These instances of vascular inflammation may contribute to the development of insulin resistance 30,31) . Insulin resistance and vascular inflammation may create a vicious cycle in vivo 7) .…”

Influence of Kiwi Fruit on Immunity and Its Anti-oxidant Effects in Mice

“…The second marker was N-epsilon-(hexanoyl)-lysine (HEL), which has attracted attention as a marker of early stage lipid peroxidation. HEL is associated with rheumatoid arthritis and arteriosclerosis (Fukuchi et al, 2008;Kageyama et al, 2008). Some recent studies have shown that the intake of foods containing anti-oxidants reduces 8-OHdG ( Thompson et al, 1999;Dhawan and Jain, 2005), but there have been only a few studies on the 8-OHdG level after kiwi fruit intake (Collins and Harrington, 2003) and no study on HEL.…”