Immunohistochemical detection of cyclin D and cyclin A in human hyperproliferative epidermis

Inohara, Shinichi; Kitano, Yukio

doi:10.1007/bf00371580

Cited by 13 publications

(15 citation statements)

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“…The main differences before and after therapy that we found regarded the expression of cyclins A and B. In psoriasis, cyclin A was highly expressed, whereas cyclin D1 when positive, was expressed, usually not strongly, in a small percentage of epidermal cell nuclei, in accordance with other studies 33,34 . It has been suggested that cyclin A expression is more related to keratinocyte proliferation, while cyclin D1 is more related to skin carcinogenesis.…”

Section: Discussionsupporting

confidence: 90%

“…It has been suggested that cyclin A expression is more related to keratinocyte proliferation, while cyclin D1 is more related to skin carcinogenesis. Cyclin D1 has been found to be expressed in preneoplastic and neoplastic skin lesions, rather than in hyperproliferative skin states 33,35 . In other studies, cyclin D1 expression was associated with epithelial hyperproliferation without altering cell differentiation 36 .…”

Section: Discussionmentioning

confidence: 93%

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Cyclin D1, B and A expression and cell turnover in psoriatic skin lesions before and after cyclosporin treatment

Miracco

Pellegrino²,

Flori³

et al. 2000

British Journal of Dermatology

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 93%

Cyclin D1, B and A expression and cell turnover in psoriatic skin lesions before and after cyclosporin treatment

Miracco

Pellegrino²,

Flori³

et al. 2000

British Journal of Dermatology

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“…Cyclin A has been reported to be also involved in the proliferation of benign regenerative liver cells (Zindy et al, 1992). In contrast, Inohara and Kitano (1994) could not find cyclin A in the cells of normal epidermis.…”

Section: Discussionmentioning

confidence: 81%

Cyclin A is associated with an unfavourable outcome in patients with non-small-cell lung carcinomas

Volm

Koomägi²,

Mattern³

et al. 1997

Br J Cancer

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Summary Specimens of formalin-fixed, paraffin-embedded non-small-cell lung carcinomas (NSCLCs; n = 187) were analysed immunohistochemically for expression of cyclin A. The analysis was intended to determine whether cyclin A has additional prognostic value for predicting patients' survival and drug response. Estimating the proliferative activity of tumours is important for the management and prognosis of tumour patients. Using flow cytometry in an earlier study, we found that patients with non-small-cell lung carcinomas (NSCLC) who had tumours with a high proliferative activity (high proportion of S and G2/M cells) had significantly shorter survival times than patients with tumours having a low proliferative activity (Volm et al, 1985(Volm et al, , 1988. Patients with ovarian carcinomas having high proliferative activity also died earlier than patients with carcinomas having low proliferative activity (Volm et al, 1985). However, there were various limitations in the earlier assays. A disadvantage of flow cytometry was that such analyses required fresh tissues and single-cell suspensions. In our earlier studies, cell cycle analyses were also not possible in all tumour samples because DNA stemlines overlapped. In contrast, immunohistochemical analysis applies to small tumour specimens and to archival material.Cell cycle progression is controlled by protein complexes composed of cyclins and cyclin-dependent kinases (Cdks); the cyclins act as regulatory molecules and the Cdks as catalytic subunits (Cordon-Cardo, 1995). The periodic appearance of the different cyclins in distinct phases of the cell cycle suggests that these proteins can also be used as markers for tissue proliferation (Dutta et al, 1995).In the present investigation, we analysed formalin-fixed, paraffin-embedded tumour sections from patients with NSCLC for expression of cyclin A. Using immunohistochemistry, we determined the relevance of cyclin A for patient survival and drug response in vitro. MATERIALS AND METHODS Patients and tumoursOne hundred and eighty-seven patients with previously untreated non-small-cell lung carcinomas (NSCLC) were admitted into this study. All patients were surgically treated in the Chest Hospital Heidelberg-Rohrbach. The minimum follow-up time is 5 years. The morphological classification of the carcinomas was conducted according to the WHO study (1981). Tumour classifications were carried out by two pathologists. Of the 187 tumours, 107 were epidermoid carcinomas, 50 adenocarcinomas and 30 large-cell carcinomas. All patients were staged at the time of their surgery according to the guidelines of the American Joint Committee on Cancer (Carr and Mountain, 1977). Thirty-seven patients had stage I, 17 had stage II and 133 had stage III tumours. The patients (167 men, 20 women) ranged in age from 28 to 76 years (average age 58 years). Seventy-one patients did not have lymph node involvement, while 115 patients had lymph node involvement (one patient could not be classified). One hundred and twenty-seven patients were treated ...

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“…A number of other cells including skin and mucosal squamous cells, transitional cells, glandular cells (breast, gastrointestinal, female and male genital tracts) and stromal cells were added to the growing list of Cyclin D1 positive cells in 1994 by Bartkova and Colleagues . An endothelial neoplasm malignant haemangioendothelioma was also shown to be reactive .…”

Section: Discussionmentioning

confidence: 99%

Immunohistological recognition of cyclin D1 expression by non-lymphoid cells among lymphoid neoplastic cells

Abdulla

Turley

Gatter

2013

APMIS

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Cyclin D1 immunostaining of non-neoplastic cells has been a source of diagnostic confusion especially in lymphoproliferative lesions. This study has reviewed these in two hundred and thirty-one haematopathological samples stained for cyclin D1. Most cases were formalin-fixed except for a few bone marrow trephines, which were B-5 fixed, and EDTA decalcified. Overall, 94% (216/231) of cases showed one or more types of non-neoplastic cells expressing Cyclin D1 of variable intensity. Endothelial cells and histiocytes were the most commonly identified Cyclin D1 positive cells being positive in 92% (214/231) of cases. Other normal cell types identified included fat cells, stromal fibroblasts, glial cells, spermatocytes, smooth muscle cells, osteoblasts and where present epithelial cells. Many normal cell types can express cyclinD1. Knowledge of these is useful to prevent misinterpretation of cyclin D1 positive tumours.

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Immunohistochemical detection of cyclin D and cyclin A in human hyperproliferative epidermis

Cited by 13 publications

References 14 publications

Cyclin D1, B and A expression and cell turnover in psoriatic skin lesions before and after cyclosporin treatment

Cyclin D1, B and A expression and cell turnover in psoriatic skin lesions before and after cyclosporin treatment

Cyclin A is associated with an unfavourable outcome in patients with non-small-cell lung carcinomas

Immunohistological recognition of cyclin D1 expression by non-lymphoid cells among lymphoid neoplastic cells

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