Immunohistochemical Detection of Aspergillus Species in Pediatric Tissue Samples

Choi, John K.; Mauger, Joanne; McGowan, Karin L.

doi:10.1309/dk1c-g9ma-tkyy-bfmq

Cited by 19 publications

(20 citation statements)

References 24 publications

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“…Similarly, Lee et al [13] reported that only 43 of 52 patients (83%) with histomorphologic aspergillosis had positive cultures for Aspergillus species, and the remaining 9 (17%) had cultures positive for other organisms including Scedosporium and Fusarium spp. Thus, our findings on the limitations of tissue morphology alone for diagnosing aspergillosis are consistent with previous findings [1,7,[11][12][13]. However, only limited data are available concerning the accuracy of histomorphologic diagnosis of mucormycosis.…”

Section: Discussionsupporting

confidence: 92%

“…Although direct comparisons are difficult because different antibodies were used in different studies, our findings are consistent with work showing that IHC could improve the sensitivity and specificity of diagnosis for systemic mycoses, especially aspergillosis [1,7,11,12]. However, there is little information on the utility of mucormycosis IHC because of the few patients with mucormycosis studied previously.…”

Section: Discussionsupporting

confidence: 84%

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Assessment of the accuracy of histomorphologic diagnosis of aspergillosis and mucormycosis by immunohistochemical tests

Jung¹,

Park²,

Sung

et al. 2015

Clin Infect Dis.

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Background. Data on the accuracy of conventional histomorphologic diagnosis are limited, especially in mucormycosis. We therefore investigated the accuracy of histomorphologic diagnosis of mucormycosis and aspergillosis, using immunohistochemistry (IHC) tests for mucormycosis and aspergillosis.Methods. Patients enrolled met the modified criteria for proven and probable mucormycosis (during a 22-year period) or invasive aspergillosis (during a 6-year period) and had formalin-fixed, paraffin-embedded tissues available. We first tested the diagnostic performance of IHC for mucormycosis and aspergillosis in proven cases. Then we determined the accuracy of histomorphologic diagnosis of probable cases, using the IHC tests.Results. In 7 proven cases of mucormycosis, the sensitivity and specificity of mucormycosis IHC were 100% (95% confidence interval, 65%-100%) and 100% (68%-100%), respectively. In 8 proven cases of aspergillosis, and the sensitivity and specificity of aspergillosis IHC staining were 87% (53%-98%) and 100% (65%-100%), respectively. Of 23 probable mucormycosis cases, 20 (87%) were positive with mucormycosis IHC, 2 (9%) were positive with aspergillosis IHC (including 1 positive for both), and 2 were negative with both. Of 16 probable aspergillosis cases, 10 (63%) were positive with aspergillosis IHC, 4 (25%) were positive with mucormycosis IHC, and 2 (13%) were negative with both tests.Conclusions. Aspergillosis and mucormycosis seem not to be correctly diagnosed morphologically, because some of the probable cases showed either test with both antibodies or failure to stain with the homologous antibody. In the absence of fungal culture results, the IHC tests seem helpful in differentiating between aspergillosis and mucormycosis.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 84%

Assessment of the accuracy of histomorphologic diagnosis of aspergillosis and mucormycosis by immunohistochemical tests

Jung¹,

Park²,

Sung

et al. 2015

Clin Infect Dis.

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“…A localização da imunomarcação nas hifas observada nos casos de zigomicose (ocorrendo principalmente no citoplasma) e aspergilose (na parede, nos septos e no citoplasma) pode estar associada ao tipo de antígeno específico de cada anticorpo. De acordo com alguns autores (Jensen et al 1996b, Choi et al 2004, anticorpos contra antígenos somáticos solúveis em água (water-soluble somatic antigens -WSSA) geralmente reagem com estruturas presentes no citoplasma (componentes não estruturais), enquanto anticorpos contra antí-genos presentes nas frações da parede (wall fraction -WF) reagem com componentes estruturais presentes principalmente na parede, septos e citoplasma. A diferenciação na localização da imunomarcação auxilia na interpretação dos resultados da técnica de imuno-histoquímica para fungos, bem como para outros agentes (Ramos-Vara et al 2008).…”

Section: Discussionunclassified

“…Alguns desses estudos têm sido publicados (Jensen et al 1994, Rocha et al 1998, Choi et al 2004, Schuetz & Cohen 2009) e são de grande valor no aprimoramento do uso da IHQ para detecção e identificação de fungos na rotina em patologia veterinária.…”

Section: Introductionunclassified

Utilização de três métodos imuno-histoquímicos na detecção de aspergilose e zigomicose em animais

et al. 2014

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Visando a otimização do uso da técnica de imuno-histoquímica (IHQ) na detecção de Aspergillus spp. e zigomicetos (membros da família Mucoraceae), utilizaram-se dois anticorpos monoclonais fungo-específicos em fragmentos de tecidos de animais (fixados em formol e embebidos em parafina) com diagnóstico histomorfológico prévio de aspergilose e zigomicose, os quais foram submetidos a três sistemas de detecção diferentes (dois biotinilados e um não biotinilado). Os dois anticorpos apresentaram alta especificidade e sensibilidade nos tecidos examinados. Não ocorreram reações cruzadas entre os anticorpos utilizados e os agentes etiológicos avaliados (incluindo casos de aspergilose, zigomicose, candidíase e pitiose). No entanto, reações inespecíficas foram observadas nas hifas em alguns casos, as quais puderam ser eliminadas através de um dos métodos de detecção utilizados. Para a aspergilose, o método da estreptavidina-biotina-fosfatase alcalina não apresentou reações inespecíficas nas hifas. Enquanto que nos casos de zigomicoses, as reações inespecíficas não ocorreram no método por polímero (não biotinilado). A técnica de IHQ mostrou-se uma ferramenta muito útil na detecção e confirmação dos casos de aspergilose e zigomicose neste estudo retrospectivo.

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“…Besides the ELISA assay, immunohistochemical galactomannan detection in FFPE tissue specimens with the aid of monoclonal antibodies is a useful tool in the diagnosis of IA [46,47].…”

Section: Galactomannanmentioning

confidence: 99%

Biopsy Procedures for Molecular Tissue Diagnosis of Invasive Fungal Infections

Mrazek

Lass‐Flörl

2011

Curr Infect Dis Rep

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The incidence of invasive fungal infections (IFI) has increased substantially and the epidemiology has changed dramatically in recent years. Candida albicans is still most important, but non-albicans species, Aspergillus species, Glomeromycota (formerly Zygomycetes) and Fusarium species are an increasing cause of IFIs. Due to this growing diversity, the identification of the causative organism to genus and species level is important to perform best and adequate treatment. The early, sensitive and specific detection of IFIs remains challenging and current conventional methods are limited. The golden standard for the definite diagnosis of proven pulmonary infection remains either histopathologic, cytopathologic or direct tissue examination. Invasive procedures are necessary to obtain reliable specimens and biopsies may be taken percutaneously, bronchoscopically, via open surgery or via video-assisted thorascopic surgery. Molecular methods, like PCR or in situ hybridization, are a promising diagnostic tool for rapid and reliable species identification and should be performed in addition to microscopic examination and culture to increase the sensitivity for the diagnosis of IFI. Combining culture, microscopy, serology, and PCR in lung tissues and/or bronchial samples will increase the diagnostic yield by 99%. Here, we give an overview of biopsy procedures for molecular tissue diagnosis of IFI.

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Immunohistochemical Detection of Aspergillus Species in Pediatric Tissue Samples

Cited by 19 publications

References 24 publications

Assessment of the accuracy of histomorphologic diagnosis of aspergillosis and mucormycosis by immunohistochemical tests

Assessment of the accuracy of histomorphologic diagnosis of aspergillosis and mucormycosis by immunohistochemical tests

Utilização de três métodos imuno-histoquímicos na detecção de aspergilose e zigomicose em animais

Biopsy Procedures for Molecular Tissue Diagnosis of Invasive Fungal Infections

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