Immunohistochemical demonstration of the nerves in human dental pulp with antisera against neurofilament protein and glia-specific S-100 protein.

Maeda, Takeyasu; Iwanaga, Toshihiko; Fujita, Tsuneo; Kobayashi, Shígeo

doi:10.1679/aohc.48.123

Cited by 30 publications

(10 citation statements)

References 19 publications

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“…There is a rather large number of immunohistological investigations on nerves in the human tooth pulp [Maeda et al, 1985[Maeda et al, , 1994Okamura et al, 1995;Egan et al, 1996;Rodd et al, 2000;Rodd and Boissonade, 2000a, b;Byers et al, 2003;Rod and Boissonade, 2003;Dourou et al, 2006;Caviedes-Bucheli et al, 2008]. However, only some older non-immunohistological studies [Graf and Björlin, 1951;Engstöm and Öhmann, 1960;Pischinger and Stockinger, 1968;Dahl and Mjör, 1973] comment on arterioles and myelinated nerves.…”

Section: Discussionmentioning

confidence: 99%

Immunostaining of Pulpal Nerve Fibre Bundle/Arteriole Associations in Ground Serial Sections of Whole Human Teeth Embedded in Technovit® 9100

Steiniger

Bubel²,

Böckler³

et al. 2013

Cells Tissues Organs

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A technique for embedding human undecalcified tooth specimens in Technovit® 9100 was developed, which permits immunohistological evaluation of pulp tissue in serial ground sections. Human molars were divided into 14-18 sections of about 23 µm thickness. Immunohistological double staining for S-100 and CD34 revealed unique associations of myelinated nerve fibre bundles with arterioles, which continued through the entire tooth pulp. These arterioles were not only accompanied by, but partially or totally enveloped in longitudinally orientated myelinated nerve fibre bundles. We speculate that this unique arrangement may mechanically support the arterioles and alleviate detection or regulation of their contraction state by sensory nerve cells.

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Section: Discussionmentioning

confidence: 99%

Immunostaining of Pulpal Nerve Fibre Bundle/Arteriole Associations in Ground Serial Sections of Whole Human Teeth Embedded in Technovit® 9100

Steiniger

Bubel²,

Böckler³

et al. 2013

Cells Tissues Organs

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“…Maeda et al [21,22] used antibodies raised against NF proteins and S-100 pro tein to demonstrate the innervation of the human tooth. Interestingly they found only Schwann cells and nerve fibres to be immunolabellcd with these antibodies.…”

Section: Discussionmentioning

confidence: 99%

Distribution of Neural and Neural-Crest-Related Antigens in Human Dental Pulp by Immunohistochemistry

Lombardi¹,

Fiore-Donno²

1993

Cells Tissues Organs

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Human teeth (formalin-fixed, demineralized and paraffin-embedded as well as frozen sections of human dental pulp) were examined by immunohistochemistry to detect the presence and distribution of a series of neural and neural-crest-associated antigens: S-100 protein, neuron-specific enolase (NSE), glial fibrillary acid protein (GFAP), myelin basic protein (MBP) and neurofilaments (NF). Although several experimental pieces of evidence support a neural crest derivation for dental papilla and odontoblasts with the histological method used in the present study, these structures were immunonegative for most of the markers employed in the present investigation. Indeed, mesenchymal cells as fibroblasts and endothelial cells were devoid of immunostaining. Schwann cells were strongly immunostained by S-100, NSE and GFAP. Nerve fibres running in the pulp showed a positive labelling for NF, S-100 and MBP antisera. It is concluded that the lack of labelling of pulp cells (excepting nerves) by the neural and neural-crest-associated antibodies could be due to a loss of capacity to express these antigens during differentiation. However, the possibility that these antigenic determinants are expressed but outside the limits of sensitivity of immunohistochemistry cannot be ruled out. Further studies, including molecular biology techniques, are required to clarify these alternatives.

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“…However, the ultrastructural interaction between the sensory nerves and odontoblasts still remains to be clarified. J Dent Res 75(12) 1996 The innervation in dentin/pulp has been welldocumented by various histological techniques: silver impregnation (Fearnhead, 1967;Gunji, 1982), selective nerve resection (Corpron et al, 1972;Arwill et al, 1973), immunohistochemical staining for endogenous substances (Wakisaka et al, 1984(Wakisaka et al, , 1987Maeda et al, 1985Maeda et al, , 1986Uddman et al, 1986;Silverman and Kruger, 1987), or the autoradiographic method (Byers and Kish, 1976;Byers, 1980;Byers et al, 1988). However, the usefulness of these techniques is usually compromised by some limitations.…”

Section: Introductionmentioning

confidence: 99%

An Ultrastructural Study of the Relationship between Sensory Trigeminal Nerves and Odontoblasts in Rat Dentin/Pulp as Demonstrated by the Anterograde Transport of Wheat Germ Agglutinin-Horseradish Peroxidase (WGA-HRP)

et al. 1996

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Because the ultrastructure of the trigeminal sensory nerves in dentin, especially in relation to odontoblasts, remains to be clarified, we investigated the relationship between the trigeminal sensory nerves and the odontoblast processes using the anterograde axonal transport technique by injecting wheat germ agglutinin-horseradish peroxidase (WGA-HRP) into the rat trigeminal ganglion. Light microscopically, the nerves labeled with WGA-HRP were mainly concentrated at the pulpal horn, forming a nerve plexus at the subodontoblastic region and penetrating the predentin/dentin about 50 to 70 microns. Ultrastructurally, HRP reaction products were observed intra-axonally in the myelinated (A delta) and unmyelinated (C) axons in the subodontoblastic region. Most nerves lost the Schwann sheath and were naked in the predentin/dentin. The labeled varicosities were close to the odontoblast processes in the dentinal tubules. No synaptic structures could be detected between the varicosities and the odontoblasts, but a gap about 20 nm wide was found between them. One type of varicosity was a rich mitochondria-containing varicosity, while the other was a rich vesicle-containing (large dense core vesicles and small clear vesicles) one. The reaction products were also found in the extracellular spaces surrounding the axons. Sometimes the reaction products were seen in the coated pits or the endocytotic vesicles of the odontoblast processes. The present study demonstrated that nerve endings (varicosities) derived from the trigeminal ganglion were present in the dentinal tubules, and that WGA-HRP extracellularly extruded from the sensory nerves in the odontoblastic layer or predentin/dentin. These findings thus suggest that sensory nerves may have some (e.g., trophic) effect on either odontoblasts or the environment around the sensory nerves in the dentin/pulp.

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Immunohistochemical demonstration of the nerves in human dental pulp with antisera against neurofilament protein and glia-specific S-100 protein.

Cited by 30 publications

References 19 publications

Immunostaining of Pulpal Nerve Fibre Bundle/Arteriole Associations in Ground Serial Sections of Whole Human Teeth Embedded in Technovit® 9100

Immunostaining of Pulpal Nerve Fibre Bundle/Arteriole Associations in Ground Serial Sections of Whole Human Teeth Embedded in Technovit® 9100

Distribution of Neural and Neural-Crest-Related Antigens in Human Dental Pulp by Immunohistochemistry

An Ultrastructural Study of the Relationship between Sensory Trigeminal Nerves and Odontoblasts in Rat Dentin/Pulp as Demonstrated by the Anterograde Transport of Wheat Germ Agglutinin-Horseradish Peroxidase (WGA-HRP)

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