Immunohistochemical demonstration of carcinoembryonic antigen.

Macartney, J. C.; Walker, Rosemary A.

doi:10.1136/jcp.32.11.1192-b

Cited by 4 publications

(4 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was necessary to preabsorb the commercial antibody, because failure to do so led to widespread staining of benign tissue. 10 The concept of CEA as an onco-developmental antigen fits well with our finding that CEA is not expressed in well-differentiated prostatic adenocarcinoma but is present in a percentage of undifferentiated tumors.…”

Section: Discussionsupporting

confidence: 83%

“…Rabbit antihuman CEA, AFP, and B-HCG: swine antirabbit immunoglobulins and PAP soluble complexes were ob- Cross-reactivity of anti-CEA serum with nonspecific cross-reacting glycoproteins was removed by absorption of antiserum with acetone dried powdered human spleen. 10 Control sections were stained in parallel and included omission of the primary antibody and substitution of the primary antibody with nonimmune normal rabbit serum in duplicate sections.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Multiple Immunoperoxidase Markers in Benign Hyperplasia and Adenocarcinoma of the Prostate

Ellis

Leffers

Davies

et al. 1984

American Journal of Clinical Pathology

View full text Add to dashboard Cite

A series of 60 cases of prostatic adenocarcinoma and 34 cases of benign prostatic hyperplasia were examined quantitatively after immunoperoxidase staining for prostate-specific antigen (PSA), prostate-specific acid phosphatase (PSAP), carcino-embryonic antigen (CEA), epithelial membrane antigen (EMA), alpha fetoprotein (AFP), and human chorionic gonadotrophin (HCG). The tumors were graded I to IV according to the MDAH grading system recently proposed. Fifty-nine of the 60 tumors were positive for PSA and 58 were positive for PSAP. The one PSA and PSAP negative case was CEA negative and weakly EMA positive. Grade I to III tumors stained more tumor cells and more diffusely for PSA and PSAP than grade IV tumors. There was no significant difference in the intensity or extent of staining between grade I and grade II-III tumors for PSA and PSAP. A comparison of PSA and PSAP showed that PSA stained more intensely and more extensively than PSAP. Benign prostatic tissue and low-grade prostatic tumors did not stain for CEA but three of the 20 grade IV tumors and one of the 23 grade II-III tumors did. Staining for EMA was focal and showed no relation to tumor grade. Benign and malignant lesions failed to stain for AFP and HCG.

show abstract

Section: Discussionsupporting

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Multiple Immunoperoxidase Markers in Benign Hyperplasia and Adenocarcinoma of the Prostate

Ellis

Leffers

Davies

et al. 1984

American Journal of Clinical Pathology

View full text Add to dashboard Cite

show abstract

“…These authors used anti-CEA from the same source (Dakopatts, Santa Barbara, U.S.A.). Others have emphasized the need for care in the interpretation of results using unabsorbed antibodies to CEA (Macartney & Walker, 1980;Nielsen, Paulsen & Johansen, 1983), and Messrs Dakopatts include a suggestion that the antiserum be pre-absorbed with spleen extract. This is, however, a tedious preparative procedure.…”

Section: Discussionmentioning

confidence: 99%

“…A commercial unpurified rabbit antibody to CEA (A15, Dakopatts, Mercia Brocades, Weybridge) that had been noted to have significant anti-CEX activity before absorption with spleen extract (Walker, 1980) was also employed.…”

Section: Methodsmentioning

confidence: 99%

Intercellular canaliculi in eccrine sweat glands: an immunoperoxidase study

et al. 1985

View full text Add to dashboard Cite

Using an indirect immunoperoxidase technique, both epithelial membrane antigen and carcinoembryonic antigen were identified within the ducts and secretory coils of the eccrine sweat gland. Antibodies to epithelial membrane antigen stained the intercellular canaliculi of the secretory coils, as did those antisera to CEA which showed activity against normal cross-reacting antigen (CEX, NCA). Those without such activity showed minimal or no staining of intercellular canaliculi. There is a difference in antigenic expression between the acinar cells and their intercellular canaliculi, and the cells of eccrine ducts.

show abstract

Tumor antigens in neoplasms of the human parotid gland

Caselitz

Seifert

Jaup

1982

J Oral Pathology Medicine

View full text Add to dashboard Cite

In a collection of parotid gland tumors the presenee of different antigens was studied by immuno-histoehemical methods. The series was eomposed of different tumors: adeno-and eystadenoeareinomas, adenoid-eystic carcinomas, salivary duct carcinomas, mucoepidermoid tumors, squamous cell carcinomas and anaplastie carcinomas.The following substances were studied: 1. Substances normally present in salivary glands like lysozyme and laetoferrin. 2. Oncofetal antigens: earcinoembryonie anligen (CEA) and alpha-fetoprotein (AFP). 3. Different elasses of intermediate-sized filaments: prekeratin and vimentin. The presenee of laetoferrin and careinoembryonic antigen could be demonstrated in the glandular differentiated tumors, whereas the squamous cell eareinomas, although CEA positive, were laetoferrin negative. The anaplastie carcinomas were negative for laetoferrin and CEA. Lysozyme and AFP could not be demonstrated in the tumors of our material. Mucoepidermoid tumors and squamous cell carcinomas were clearly positive for prekeratin filaments whereas the stromal part showed vimentin filaments in the eytoplasm of fibroblasts. These antigens provide a useful tool lo distinguish between the epithelial and mesenchymal tumors.

show abstract

Immunohistochemical demonstration of carcinoembryonic antigen.

Cited by 4 publications

References 12 publications

Multiple Immunoperoxidase Markers in Benign Hyperplasia and Adenocarcinoma of the Prostate

Multiple Immunoperoxidase Markers in Benign Hyperplasia and Adenocarcinoma of the Prostate

Intercellular canaliculi in eccrine sweat glands: an immunoperoxidase study

Tumor antigens in neoplasms of the human parotid gland

Contact Info

Product

Resources

About