Intercellular canaliculi in eccrine sweat glands: an immunoperoxidase study

Graham, Robert M.; McKee, Phillip H.; Chapman, David C.; Richardson, T. C.; Stokoe, M.R.; Heyderman, Eadie

doi:10.1111/j.1365-2133.1985.tb02312.x

Cited by 12 publications

(4 citation statements)

References 20 publications

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“…However, subsequent studies demonstrated immunoreactivity for EMA in a variety of simple epithelia that have secretory functions as well as in carcinoma of different origin (4,5). Beyond that, staining of sweat glands and sebaceous glands could be demonstrated (7,(12)(13)(14). In some neoplasms with sweat gland differentiation, the expression of EMA proved to be highly conserved (15)(16)(17).…”

mentioning

confidence: 99%

Ultrastructural localization of carcinoembryonic antigen (CEA) glycoproteins and epithelial membrane antigen (EMA) in normal and neoplastic sweat glands

Metze

Luger

1996

J Cutan Pathol

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Glycoproteins of the carcinoembryonic antigen family (CEA) and epithelial membrane antigen (EMA) are established markers for glandular and mucosal tissues. However, their precise ultrastructural distribution in sweat glands has not been determined as yet. Therefore, normal human skin, 19 cases of various sweat gland neoplasms, Paget's disease, and cutaneous metastases of visceral carcinomas were stained with well-defined antibodies using a postembedding immunogold technique. In some cases, a new method of re-embedding paraffin material for immunoelectron microscopy was applied. In normal sweat glands, immunoreactivity of the endoplasmic reticulum and vesicles indicated biosynthesis and processing of CEA and EMA. Along the luminal surfaces both CEA and EMA represented an integral part of microvilli. However, a differential expression of CEA and EMA was demonstrated in apocrine epithelia, mucous cells of eccrine glands, and sweat ducts. In fetal glands, CEA was associated with formation of secretory and ductal lumina. The overall cellular distribution of CEA and EMA was highly preserved in benign sweat gland neoplasms whereas malignant neoplasms were characterized by loss of protein targeting and cellular polarity. In conclusion, these immunoelectron microscopical findings suggest a role of CEA and EMA for cell differentiation and secretory mechanisms of sweat gland epithelia.

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mentioning

confidence: 99%

Ultrastructural localization of carcinoembryonic antigen (CEA) glycoproteins and epithelial membrane antigen (EMA) in normal and neoplastic sweat glands

Metze

Luger

1996

J Cutan Pathol

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“…Use of both the anti-rabbit and anti-mouse immunoglobulin peroxidase conjugates in a number of previous studies (Heyderman, 1983;1984a, b;Graham et al, 1985) had indicated that neither of them showed evidence of anti-human activity. Neither of them stained tissue sections when an inappropriate or absorbed negative control serum had been used.…”

mentioning

confidence: 99%

A new monoclonal antibody to epithelial membrane antigen (EMA)-E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2

Heyderman¹,

Strudley²,

Powell³

et al. 1985

Br J Cancer

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Summary Two polyclonal rabbit antibodies to epithelial membrane antogen (EMA), two mouse monoclonal antibodies (E29 and HMFG-2), and a "cocktail" of these two monoclonals have been compared using an indirect immunoperoxidase technique. Sections from 25 tissues (17 malignant and 8 benign), were examined. The distribution of staining with each of these reagents was similar, but the polyclonal antibodies produced stronger staining in colorectal carcinomas and lactating breast, whereas staining with the monoclonal antibodies was stronger in non-neoplastic pleural mesothelium and in pulmonary alveolar cells. When the two monoclonals were mixed there was no increase in staining intensity. E29 gave a "cleaner" result than HMFG-2, with better discrimination between cells and stroma, and is highly suitable for routine diagnostic histopathology.

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“…The most intensely staining monoclonal antibody, DD9E7, identified an antigen which was present in large amounts in 12/14 of pancreatic adenocarcinomas, absent from islets and variably expressed on normal acini ducts and ductules. The antigen was also found in colon and breast carcinomas and a number of normal tissues but its immunohistochemical pattern was different to that found with antibodies against CEA or EMA (Heyderman et al, 1979;Grahame et al, 1985). Consistent staining of polymorphs and macrophages in all sections suggested the antigen may be an NCA-like material (Mach & Putztnaseri, 1972;Von Kleist et al, 1972).…”

Section: Resultsmentioning

confidence: 68%

The generation of monoclonal antibodies against human pancreatic exocrine cancer: A study of six different immunisation regimes

Grant¹,

Harris²,

Heyderman³

et al. 1985

Br J Cancer

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Six different immunisation regimes have been used to generate spleen cells with reactivity against human pancreatic exocrine cancer. Immunised spleen cells were fused with an NSO/1 myeloma line and supernatants from these hybridomas selectively screened for monoclonal antibodies which bound predominantly to a pancreatic cancer cell line (GER). The spleen cells from hairy litter mates immunised with pancreatic cancer xenograft homogenates and viable GER cells generated 13% of hybridoma supernatants which showed some selectivity for GER pancreatic cancer cells in a fixed cell ELISA assay. The other methods produced only 4% of hybrids with selectivity for GER cells. The antigen distribution on gluteraldehyde fixed cells was similar to that found for viable cell monolayers but many antigens were unstable on formalin fixation. Immunohistochemical staining of GER cells grown on glass slides showed a heterogeneity of antigen distribution with up to 70% of the cells exhibiting a vesicular pattern of staining. Fifty percent of the antibodies which bound to GER cells were also reactive against antigens in formalin-fixed paraffin-embedded tissue sections of the original GER tumour. Monoclonal antibody DD9E7 identified an antigen expressed on 12/14 pancreatic adenocarcinomas. The antibody showed strong staining of malignant luminal membranes and cytoplasm. The antigen was also present in normal salivary and sweat glands, and colon and breast carcinomas, but its tissue distribution was unlike that of CEA or EMA. The expression of this antigen in 12/14 of pancreatic carcinomas suggests that DD9E7 may be a useful reagent for pancreatic tumour detection. Images Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6

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Intercellular canaliculi in eccrine sweat glands: an immunoperoxidase study

Cited by 12 publications

References 20 publications

Ultrastructural localization of carcinoembryonic antigen (CEA) glycoproteins and epithelial membrane antigen (EMA) in normal and neoplastic sweat glands

Ultrastructural localization of carcinoembryonic antigen (CEA) glycoproteins and epithelial membrane antigen (EMA) in normal and neoplastic sweat glands

A new monoclonal antibody to epithelial membrane antigen (EMA)-E29. A comparison of its immunocytochemical reactivity with polyclonal anti-EMA antibodies and with another monoclonal antibody, HMFG-2

The generation of monoclonal antibodies against human pancreatic exocrine cancer: A study of six different immunisation regimes

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