Immunohistochemical Demonstration of an Organized Cytoarchitecture of the Radial Glia in the CNS of the Embryonic Mouse

Dupouey, P.; Benjelloun, Soumaya; Gomès, Danielle

doi:10.1159/000112279

Cited by 63 publications

(38 citation statements)

References 23 publications

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“…In vimentin-null mutant mice, despite the marked disruption of BG development and the disturbance of the regular glial scaffolding normally present in young animals (Dupouey et al, 1985), granule cell migration does not seem to be impaired, thus substantiating the view that the apposition of granule neurons on intact BG processes is not essential for neuronal migration and suggesting also that the geometry of BG does not play a crucial role in directing that migration. Therefore, it could be hypothesized, as expressed earlier by Sotelo and Changeux (1974) and Privat et al (1979), that this regular glial scaffolding offers a preferential axis for migration, whereas when this geometry is disturbed at the time of granule cell migration, pioneer migrating neurons can set up alternative axes, which are then used by followers.…”

Section: Discussionmentioning

confidence: 56%

Cerebellar defect and impaired motor coordination in mice lacking vimentin

Colucci‐Guyon

Ribotta

Maurice

et al. 1999

Glia

105

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Section: Discussionmentioning

confidence: 56%

Cerebellar defect and impaired motor coordination in mice lacking vimentin

Colucci‐Guyon

Ribotta

Maurice

et al. 1999

Glia

105

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“…Antibodies to vimentin, an intermediate filament protein found in radial glial cells in late embryogenesis (16), stained the same columns as labeled by the oncogene antibodies in adjacent sections (Fig. 4 A and B).…”

mentioning

confidence: 62%

“…Although radial glial cells are capable of division (13), some remain mitotically dormant during late embryogenesis (14). Radial glial cells' fibers are thought to aid in the prenatal migration of young postmitotic neurons (15,16). Their cell bodies are juxtaposed to the ventricle and a long process extends to the outer surface of the developing brain (17).…”

Section: Introductionmentioning

confidence: 99%

Protooncogene expression identifies a transient columnar organization of the forebrain within the late embryonic ventricular zone.

Johnston

Kooy

1989

Proc. Natl. Acad. Sci. U.S.A.

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Immunocytochemical studies using monoclonal antibodies directed against oncogenic peptides revealed a heterogeneous distribution of the peptides within the ventricular zone of the embryonic day 18 rat forebrain. The sis-, src-, ras-, and myc-encoded peptides were concentrated in the same isolated clusters of 5-25 radial glial cells (also identified by vimentin staining), providing a transient columnar compartmentalization to the ventricular zone. An increased number of [3lHlthymidine-labeled ventricular zone cells were observed within the protooncogene stained radial glial cell columns as compared to noncolumn areas. The columnar heterogeneity of radial glial cells reveals the mosaicism of the embryonic ventricular zone and the differential proliferation of its cells.The mammalian telencephalon develops embryonically from the ventricular zone and at later stages from the subventricular zone, which are proliferative regions of the developing brain (1). Within the apparently homogeneous cells of the ventricular zone (2), there are few markers of the commitments made by cells to their eventual differentiated fates (3). Indeed, the question of whether neuronal fate is determined in the ventricular zone or postmitotically (after they migrate out of this zone) is under current debate. Protooncogenes, the cellular homologues of oncogenes, have been shown to be expressed in different stages of embryonic development in a variety of tissues (4, 5). Available evidence suggests that the action of protooncogene products on cells can be to increase mitotic activity and to induce differentiation (6). The purpose of the present study was to determine the distribution of protooncogenes within the developing forebrain of the embryonic rat, as possible markers of selective proliferation or differentiation of brain cells. The involvement of protooncogenes in neuronal development has been previously investigated in the developing cerebellum and retina, where the src oncogene has been localized to postmitotic neurons in both of these structures (7,8).The protooncogenes sis [a homologue of the B chain of platelet-derived growth factor (9)], src [a tyrosine kinase (10)], ras [a GTP binding protein (11)], and myc [a DNA binding protein (12)] represent members of different classes of protooncogenes. In the present study, these protooncogenes were found to be expressed in the same isolated clusters of radial glial cells in the ventricular zone. Although radial glial cells are capable of division (13), some remain mitotically dormant during late embryogenesis (14). Radial glial cells' fibers are thought to aid in the prenatal migration of young postmitotic neurons (15,16). Their cell bodies are juxtaposed to the ventricle and a long process extends to the outer surface of the developing brain (17). The regional and time-dependent expression of certain protooncogenes in specific columns of radial glial cells, along with associated changes in vimentin expression and the proliferation of nearby cells, reveal a heterogeneous organizati...

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“….Alternatively, GATl mRNA (Rattray and Priestley, 1993;Durkin et al, 1995) and its polypeptide (Radian et al, 1990) have been localized in Bergmann glia in adult rats. These cells are present in the mouse cerebellar anlage from E l 5 onward (del Cerro and Swarz, 1976) and are aligned with Purkinje cell bodies shortly after birth (Dupouey et al, 1985). It also is possible that, during early cerebellar development, some component of the mGATl signal in the cerebellar cortex is localized in Golgi cells.…”

Section: Discussionmentioning

confidence: 99%

Embryonic and postnatal expression of four gamma-aminobutyric acid transporter mRNAs in the mouse brain and leptomeninges

1996

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The distribution of gamma-aminobutyric acid (GABA) transporter mRNAs (mGATs) was studied in mouse brain during embryonic and postnatal development using in situ hybridization with radiolabeled oligonucleotide probes. Mouse GATs 1 and 4 were present in the ventricular and subventricular zones of the lateral ventricle from gestational day 13. During postnatal development, mGAT1 mRNA was distributed diffusely throughout the brain and spinal cord, with the highest expression present in the olfactory bulbs, hippocampus, and cerebellar cortex. The mGAT4 message was densely distributed throughout the central nervous system during postnatal week 1; however, the hybridization signal in the cerebral cortex and hippocampus decreased during postnatal weeks 2 and 3, and in adults, mGAT4 labeling was restricted largely to the olfactory bulbs, midbrain, deep cerebellar nuclei, medulla, and spinal cord. Mouse GAT2 mRNA was expressed only in proliferating and migrating cerebellar granule cells, whereas mGAT3 mRNA was absent from the brain and spinal cord throughout development. Each of the four mGATs was present to some degree in the leptomeninges. The expression of mGATs 2 and 3 was almost entirely restricted to the pia-arachnoid, whereas mGATs 1 and 4 were present only in specific regions of the membrane. Although mGATs 1 and 4 may subserve the classical purpose of terminating inhibitory GABAergic transmission through neuronal and glial uptake mechanisms, GABA transporters in the pia-arachnoid may help to regulate the amount of GABA available to proliferating and migrating neurons at the sub-pial surface during perinatal development.

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Immunohistochemical Demonstration of an Organized Cytoarchitecture of the Radial Glia in the CNS of the Embryonic Mouse

Cited by 63 publications

References 23 publications

Cerebellar defect and impaired motor coordination in mice lacking vimentin

Cerebellar defect and impaired motor coordination in mice lacking vimentin

Protooncogene expression identifies a transient columnar organization of the forebrain within the late embryonic ventricular zone.

Embryonic and postnatal expression of four gamma-aminobutyric acid transporter mRNAs in the mouse brain and leptomeninges

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