Immunohistochemical characterization of the mononuclear cells infiltrating muscle of patients with inflammatory and noninflammatory myopathies

Giorno, Ralph; Barden, Michael T.; Köhler, Peter F.; Ringel, Steven P.

doi:10.1016/0090-1229(84)90026-6

Cited by 41 publications

(15 citation statements)

References 19 publications

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“…Em contrapartida, Giorno e col. relataram predomínio de linfócitos CD4+ nas miopatias inflamatórias 43 . Em relação à localização, nos nossos casos o infiltrado foi predominantemente intersticial, ao invés da concentração endomisial (intra-fibra) relatada por outros estudos 9,10 .…”

Section: Macrófagounclassified

Análise imunocitoquímica do infiltrado inflamatório na miosite com corpos de inclusão citoplasmática e em outras doenças neuromusculares com vacúolos marginados

Scola

Werneck

Iwamoto³

et al. 1998

Arq. Neuro-Psiquiatr.

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RESUMO -Encontramos 16 casos com vacúolos marginados entre 1400 biópsias musculares cujo diagnóstico final foi miosite com corpos de inclusão citoplasmática esporádica (MCIC) (4 casos), atrofia muscular espinhal juvenil (6 casos), miopatias distais (3 casos), distrofia das cinturas pélvica e escapular (2 casos) e neuropatia periférica (1 caso). Foram utilizados anticorpos monoclonais contra linfócitos T totais e subpopulações (CD4+ e CD8+), linfócitos B, macrófagos, células exterminadoras naturais (NK), imunoglobulinas e porção C3 do complemento. A análise foi quantitativa e de acordo com o local de acúmulo (interstício, intra-fibra e perivascular). Linfócitos CD8+ foram encontrados no interstício na maioria dos casos, sendo menos comuns dentro das fibras musculares e raros no espaço perivascular. Os casos de MCIC apresentaram maior número de linfócitos CD8+ se comparados às outras doenças. A proporção de células CD8+/CD4+ foi maior na MCIC do que nas outras doenças. Existiam macrófagos em grande proporção na MCIC, miopatias distais e em um dos casos de distrofia das cinturas pélvica e escapular. Células NK foram frequentes no interstício nos casos de MCIC e mais raras nas outras doenças. Houve maior depósito de imunoglobulinas e complemento nos casos de MCIC do que nas demais doenças. O grande número de células CD8+ e a relação CD8+/CD4+ podem auxiliar no diagnóstico diferencial da MCIC de outras doenças neuromusculares com vacúolos marginados. PALAVRAS-CHAVE: imunocitoquímica, vacúolos marginados, inclusão citoplasmática, miosite com corpos de inclusão citoplasmática, atrofia muscular espinhal juvenil, miopatias distais, distrofia muscular das cinturas pélvica e escapular. Immunocytochemical analysis of the inflammatory infiltrate in inclusion body myositis and other neuromuscular disorders with rimmed vacuolesABSTRACT -Among 1400 muscle biopsies, we found 16 cases with rimmed vacuoles whose diagnosis were sporadic inclusion body myositis (IBM) (4 cases), juvenile spinal muscular atrophy (6 cases), distal myopathies (3 cases), limb-girdle muscular dystrophy (2 cases), and peripheral neuropathy (1 case). Monoclonal antibodies reactive for T lymphocytes and subsets, B lymphocytes, macrophages, natural killer cells, immunoglobulins, and complement were used to analyze the inflammatory infiltrate. The analysis was quantitative and according to the site of accumulation (interstitial, endomysial, and perivascular). The immunocytochemical analysis showed CD8+ lymphocytes in the interstitial in most cases, occasionally inside of muscle fibers, and rarely in the perivascular region. The IBM cases had an increased number of CD8+ lymphocytes comparing with the other diseases. CD8+/CD4+ ratio was increased in IBM compared with the other diseases. Macrophages were frequent in IBM, distal myopathy, and one case of limb-girdle muscular dystrophy. Natural killer cells were frequent at interstitial Serviço de Doenças

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Section: Macrófagounclassified

Análise imunocitoquímica do infiltrado inflamatório na miosite com corpos de inclusão citoplasmática e em outras doenças neuromusculares com vacúolos marginados

Scola

Werneck

Iwamoto³

et al. 1998

Arq. Neuro-Psiquiatr.

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“…Studies of in vitro cytotoxicity of mononuclear cells from patients with PM/DM for muscle have been conflicting (12)(13)(14)(15)(16)(17), but such cells do show increased reactivity to skeletal muscle as assessed by proliferation assays (12,18) and lymphokine production (13,19). Additionally, characterization of cells in inflammatory infiltrates of muscle show T cells to be active participants in muscle fiber damage (20)(21)(22)(23). Despite this evidence, however, the factor that initiates these mechanisms is poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Coxsackievirus B 1-induced polymyositis. Lack of disease expression in nu/nu mice.

Ytterberg¹,

Mahowald²,

Messner³

1987

J. Clin. Invest.

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Chronic inflammatory myositis similar to human polymyositis occurs in mice after infection with a strain of Coxsackievirus B 1 (CVB 1). To investigate the role of T cells in the pathogenesis of this disorder, we compared disease expression in T cell-deficient athymic nude (nu/nu) mice and heterozygotes (nu/+) with normal T cell function. Acute infectious myositis occurred in nu/ nu and nu/+ mice. Chronic (> 21 d postinfection) weakness and myositis, however, developed only in nu/+. Resistance to disease in nu/nu mice was not explained by insusceptibility to infection; the amount of virus lethal for 50% of mice and virus replication were comparable in both groups. Additionally, anti-CVB 1 antibody production was similar in both groups. Reconstitution of infected nu/nu mice with spleen cells from normal mice resulted in disease. These results demonstrate that chronic weakness after infection with this virus is not simply a sequela of acute myonecrosis and suggest that T cells play a pivotal role in the pathogenesis of chronic myositis.

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“…Only recently have monoclonal antibodies become available which can distinguish var ious types of MPS cells [Giorno, 1986], A further characteristic of a cell-mediated reaction is the presence of activated cells. Initial studies in IM indicated that some T cells are activated [Giorno et al, 1984;Arahata and Engel, 1984], Such a conclusion is tentative, however, since the HLA-DR an tigen used as a marker of activation is constitutively expressed on MPS cells and B cells [Lampson and Levy, 1980] which are present in the infiltrate in IM [Giorno et al, 1984;Arahata and Engel, 1984],…”

Section: Introductionmentioning

confidence: 99%

“…The inflammatory cell infiltrate in in flammatory myopathies (IM) consists of a mixture of cells, including lymphocytes and macrophages [Giorno et al, 1984;Arahata and Engel, 1984;Lemoine et al, 1986]. T lymphocytes predominate, suggesting that a cell-mediated immune reaction may be oc curring [Giorno et al, 1984;Arahata and Engel, 1984;Lemoine et al, 1986], In cellmediated reactions, cells capable of present ing antigen must be present along with po tential effector cells.…”

Section: Introductionmentioning

confidence: 99%

“…T lymphocytes predominate, suggesting that a cell-mediated immune reaction may be oc curring [Giorno et al, 1984;Arahata and Engel, 1984;Lemoine et al, 1986], In cellmediated reactions, cells capable of present ing antigen must be present along with po tential effector cells. Macrophages as well as other cells of the mononuclear phagocyte system (MPS), which may function as anti gen-presenting cells, possess characteristic immunophenotypes and reside in particular areas of lymphoid tissues [Giorno, 1986].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of Macrophages, Activated Cells and T Cell Subsets in Inflammatory Myopathies using Monoclonal Antibodies

Giorno¹,

Ringel²

1986

Path Immunopathol Res

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Immunohistochemical characterization of the mononuclear cells infiltrating muscle of patients with inflammatory and noninflammatory myopathies

Cited by 41 publications

References 19 publications

Análise imunocitoquímica do infiltrado inflamatório na miosite com corpos de inclusão citoplasmática e em outras doenças neuromusculares com vacúolos marginados

Análise imunocitoquímica do infiltrado inflamatório na miosite com corpos de inclusão citoplasmática e em outras doenças neuromusculares com vacúolos marginados

Coxsackievirus B 1-induced polymyositis. Lack of disease expression in nu/nu mice.

Analysis of Macrophages, Activated Cells and T Cell Subsets in Inflammatory Myopathies using Monoclonal Antibodies

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