Analysis of Macrophages, Activated Cells and T Cell Subsets in Inflammatory Myopathies using Monoclonal Antibodies

Giorno, Ralph; Ringel, Steven P.

doi:10.1159/000157034

Cited by 6 publications

(3 citation statements)

References 20 publications

(32 reference statements)

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“…Immunohistological studies have demonstrated that T cells, B cells and macrophages infiltrate to the muscular lesions in DM 3 . Furthermore, IL‐2R‐expressing cells accumulate in muscular lesions of DM 11 . In contrast, the number of peripheral blood CD3 + /CD25 + cells showed no significant change after treatment (data not shown).…”

Section: Discussionmentioning

confidence: 94%

Elevated serum levels of soluble interleukin‐2 receptor in juvenile dermatomyositis

Kobayashi

Ono

Kawamura

et al. 2001

Pediatrics International

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Section: Discussionmentioning

confidence: 94%

Elevated serum levels of soluble interleukin‐2 receptor in juvenile dermatomyositis

Kobayashi

Ono

Kawamura

et al. 2001

Pediatrics International

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“…Infiltrating T‐cells are a prominent feature of most inflammatory myopathy subtypes. For example, perimysial and perivascular accumulations of T‐cells are characteristic of DM, and the presence of CD8+ T‐cells surrounding and invading nonnecrotic fibers has been described as a hallmark of PM . In the anti‐HMGCR myopathy patients studied here, we found very few examples of the former and no examples of CD8+ T‐cells surrounding and invading nonnecrotic fibers.…”

Section: Disussionmentioning

confidence: 52%

The composition of cellular infiltrates in anti‐HMG‐CoA reductase‐associated myopathy

et al. 2015

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Objective To characterize cellular infiltrates in muscle biopsies from patients with anti-3-hydroxy-3-methyl-gulatryl-CoA reductase (HMGCR)-associated myopathy. Methods Biopsies from 18 anti-HMGCR myopathy and 7 control dermatomyositis patients were analyzed. Results CD4+ and CD8+ T-cells were scattered within the endomysium in 50% of anti-HMGCR biopsies. All anti-HMGCR biopsies included increased endomysial and/or perivascular CD163+ M2 macrophages; CD11c+ M1 macrophages were present in 18.8%. CD123+ plasmacytoid dendritic (PD) cells were observed within the endomysium and perivascular spaces in 62.5% of anti-HMGCR biopsies. Membrane attack complex was deposited on endothelial cells in 50% and on the sarcolemma of non-necrotic muscle fibers in 85.7% of anti-HMGCR cases. Major histocompatibility complex class I antigen was up-regulated in 87.5% of the anti-HMGCR cases. Conclusion In addition to necrosis, scattered CD4+, CD8+, and PD cells are characteristic of anti-HMGCR myopathy. Predominant M2 polarization suggests infiltrating macrophages are more likely to be involved with tissue repair than destruction.

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“…Frozen tissue sections were stained with murine monoclonal antibodies, using an avidin-biotin immunoperoxidase method as previously described (12). The monoclonal antibodies used were anti-CD3 (Leu-4), anti-CD4 (Leu-3), anti-CD8 (Leu-2), anti-CD22 (Leu-14), and anti-CD14 (Leu-M5).…”

mentioning

confidence: 99%

inflammatory myopathy and acquired immunodeficiency syndrome

Nordstrom

Petropolis

Giorno

et al. 1989

Arthritis & Rheumatism

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A 33-year-old black woman with advanced acquired immunodeficiency syndrome (AIDS) presented with rapidly progressive muscle weakness and serologic and radiologic evidence of central nervous system Toxoplasma infection. Muscle biopsy revealed an inflammatory infiltrate predominantly composed of macrophages and T suppressorkytotoxic cells. Human immunodeficiency virus major core protein (p24) was also detected in macrophages and damaged muscle cells around the inflammatory infiltrates. The patient improved clinically with glucocorticoid therapy for polymyositis and pyrimethamine and clindamycin therapy for toxoplasmosis.In patients with acquired immunodeficiency syndrome (AIDS) or AIDS-related complex (ARC), the musculoskeletal system may be involved secondary to peripheral neuropathy, by direct infection, or by an autoimmune inflammatory process (1-9). The etiology of polymyositis in patients with acquired immunodeficiency is unknown, although other autoimmuneThe opinions and assertions contained herein are the private views of the authors and are not to be construed as official or as reflecting the views of the Department of the Army or the Department of Defense.

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Analysis of Macrophages, Activated Cells and T Cell Subsets in Inflammatory Myopathies using Monoclonal Antibodies

Cited by 6 publications

References 20 publications

Elevated serum levels of soluble interleukin‐2 receptor in juvenile dermatomyositis

Elevated serum levels of soluble interleukin‐2 receptor in juvenile dermatomyositis

The composition of cellular infiltrates in anti‐HMG‐CoA reductase‐associated myopathy

inflammatory myopathy and acquired immunodeficiency syndrome

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