Immunohistochemical Assessment of TNFAIP3/A20 Expression Correlates With Early Tumorigenesis in Breast Cancer

Sharif-Askari, Fatemeh Saheb; Alkhayyal, Noura; Talaat, Iman M.; Sharif-Askari, Narjes Saheb; Rawat, Surendra; Jundi, Majd; Syrjänen, Karí; Hamoudi, Rifat; Bendardaf, Riyad

doi:10.21873/anticanres.14825

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…As the phosphorylation gene of MAPK protein, NRAS has become one of the taxonomic markers of four subtypes of melanoma [44]; moreover, high expression of NRAS is associated with poor prognosis of lung adenocarcinoma [45]. Tumor extracellular vesicles carrying miR-125b-5p enter diffuse large B-cell lymphoma (DLBCL) cells and target TNFAIP3, thereby reducing the sensitivity of DLBCL to rituximab [46], and overexpression of TNFAIP3 is associated with a lower survival rate in breast cancer patients [47]; a study about ferroptosis after cerebral hemorrhage also find TNFAIP3 upregulation [48]. Our model predicted TSCC patient survival in the validation cohorts at 1 year (AUC = 0.889) and 32 months (AUC = 0.9).…”

Section: Discussionmentioning

confidence: 99%

Identification of ferroptosis‐related genes as potential biomarkers of tongue squamous cell carcinoma using an integrated bioinformatics approach

et al. 2021

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Section: Discussionmentioning

confidence: 99%

Identification of ferroptosis‐related genes as potential biomarkers of tongue squamous cell carcinoma using an integrated bioinformatics approach

et al. 2021

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“…A study from the UAE found that A20 expression evaluated through immunohistochemistry (IHC) was associated with early grade 1 BC (p < 0.001) in all molecular subtypes, suggesting its use as a biomarker for early cancer. A20 overexpression was also associated with a lower OS rate in patients treated with endocrine therapy [60]. Another study found that insulin-like growth factor 1 receptor (IGF1R) membranous and mixed (membranous and cytoplasmic) expression in BC cells was evident in HR + HER2− cases in contrast with HR−HER2+ cases, which showed cytoplasmic or diminished IGF1R expression, suggesting that luminal subtypes may benefit from targeted IGFR therapy [61].…”

Section: Other Biomarkersmentioning

confidence: 99%

Breast Cancer in the Arabian Gulf Countries

Al-Shamsi,

Abdelwahed,

Abyad

et al. 2023

Cancers

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Breast cancer stands as the prevailing malignancy across all six Gulf Cooperation Council (GCC) nations. In this literature review, we highlighted the incidence and trend of breast cancer in the GCC. Most of the studies reported a consistent increase in breast cancer incidence over the past decades, which was particularly attributed to the adoption of a Westernized lifestyle in the region and the implications of emerging risk factors and other environmental and societal factors, the increase in screening uptake, as well as the improvement in data collection and reporting in the GCC. The data on breast cancer risk factors in the GCC were limited. In this geographic region, breast cancer frequently manifests with distinctive characteristics, including an early onset, typically occurring before the age of 50; an advanced stage at presentation; and a higher pathological grade. Additionally, it often exhibits more aggressive features such as human epidermal growth factor receptor 2 (HER2) positivity or the presence of triple-negative (TN) attributes, particularly among younger patients. Despite the growing body of literature on breast cancer in the GCC, data pertaining to survival rates are, regrettably, meager. Reports on breast cancer survival rates emanating from the GCC region are largely confined to Saudi Arabia and the United Arab Emirates (UAE). In the UAE, predictive modeling reveals 2-year and 5-year survival rates of 97% and 89%, respectively, for the same period under scrutiny. These rates, when compared to Western counterparts such as Australia (89.5%) and Canada (88.2%), fall within the expected range. Conversely, Saudi Arabia reports a notably lower 5-year survival rate, standing at 72%. This disparity in survival rates underscores the need for further research directed toward elucidating risk factors and barriers that hinder early detection and screening. Additionally, there is a pressing need for expanded data reporting on survival outcomes within the GCC. In sum, a more comprehensive and nuanced understanding of breast cancer dynamics in this region is imperative to inform effective strategies for prevention, early detection, and improved patient outcomes.

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“…In recent years, it has been reported that A20 is involved in the pathological progression of breast cancer. Sharif-Askari et al (18) found that A20 could serve as a biomarker for early diagnosis of breast cancer. Song et al (19) confirmed that A20 was involved in inflammation-mediated metastatic disease in triple-negative breast cancer.…”

Section: Introductionmentioning

confidence: 99%

A20 affects macrophage polarization through the NLRP3 inflammasome signaling pathway and promotes breast cancer progression

et al. 2023

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Breast cancer is the most common malignant tumor in females, and the majority of patients succumb to metastasis. The present study aimed to investigate the association between tumor necrosis factor alpha-induced protein 3 (A20), NOD-, LRR-and pyrin domain-containing protein 3 (NLRP3) and tumor-associated macrophage polarization, and their effects on the proliferation and metastasis of breast cancer cells. The expression of A20 in breast cancer cells was analyzed by reverse transcription-quantitative PCR (RT-qPCR) and western blotting. RT-qPCR and western blotting were also used to confirm the transfection efficiency. The viability, clone formation, migration, invasion and angiogenesis of transfected breast cancer cells were detected by Cell Counting Kit-8, colony formation, wound healing, Transwell and tube formation assays, respectively. Activated macrophages, namely M1 and M2 type macrophages, were observed by double staining immunofluorescence. The levels of M1 and M2 macrophage markers were analyzed by qPCR. The expression of angiogenesis-related proteins and NLRP3 inflammasome activation-associated proteins was detected by western blotting. The results revealed that A20 was highly expressed in breast cancer cells. Interference with A20 inhibited the proliferation, invasion, migration and angiogenesis of breast cancer cells, and inhibited the M2-like polarization of macrophages. Interference with A20 promoted the activation of the NLRP3 inflammasome. The NLRP3 inhibitor MCC950 alleviated the effect of interference with A20 to promote macrophage proliferation and recruitment, as well as M2-like polarization. In conclusion, interference with A20 inhibited macrophage proliferation and M2-like polarization through the NLRP3 inflammasome signaling pathway to inhibit breast cancer progression.

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Immunohistochemical Assessment of TNFAIP3/A20 Expression Correlates With Early Tumorigenesis in Breast Cancer

Cited by 3 publications

References 15 publications

Identification of ferroptosis‐related genes as potential biomarkers of tongue squamous cell carcinoma using an integrated bioinformatics approach

Identification of ferroptosis‐related genes as potential biomarkers of tongue squamous cell carcinoma using an integrated bioinformatics approach

Breast Cancer in the Arabian Gulf Countries

A20 affects macrophage polarization through the NLRP3 inflammasome signaling pathway and promotes breast cancer progression

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