Immunohistochemical analysis of stromal fibrocytes and myofibroblasts to envision the invasion and lymph node metastasis in oral squamous cell carcinoma

Abstract

Background:Tumor cells work in close coordination with stromal elements from its stage of emergence to metastasis. The study was designed to assess the presence and distribution pattern of stromal fibrocytes and myofibroblasts in oral squamous cell carcinoma (OSCC). Possibility of using these stromal cells as a marker for invasion and lymphnode metastasis was evaluated.Materials and Methods:A total of 40 cases of OSCC consisting twenty cases of each lymph node positive (pN+) and lymph node negative (pN0) samples and ten normal oral mucosa (NOM) tissues were subjected to double immunostaining using CD34 and alpha-smooth muscle actin (α-SMA) antibodies. Stained sections were evaluated semiquantitatively.Results:CD34 fibrocytes were seen in 70% of NOM and none of OSCC samples. α-SMA myofibroblasts were seen in 80% of OSCC and none of NOM samples. A statistically significant difference was found in fibrocyte values (P < 0.001) and myofibroblast values (P < 0.001) between NOM and OSCC study samples. No statistical significance in myofibroblast values between pN0 and pN+ study groups; however, their distribution pattern appreciably varied.Conclusions:This study suggested that fibrocytes could be used as one of the markers for early invasion. Abrupt loss of fibrocytes at the transition zone toward carcinoma and statistical significance in their values supported this inference. Heterogeneity in the distribution pattern of myofibroblasts in tumor stroma indicates that this variability may predict the tumor behavior toward nodal metastasis rather than their mere presence or absence.

Naa10p and IKKα interaction regulates EMT in oral squamous cell carcinoma via TGF‐β1/Smad pathway

“…The cell migration and invasion assays were performed in the 24‐well Transwell plates (0.8‐m pore size filter; 3422; Corning) as follows: CALl27 and SCC15 cells were transfected with shNaa10p or IKKα siRNA for 24 hours with or without TGF‐β1 treatment, and 5 × 10 4 cells suspended in 200 µL serum‐free medium were added to the upper chamber. The lower chamber was filled with 800 µL DMEM culture medium containing 10% FBS (04‐001; Biological Industries, Kibbutz Beit HaEmek).…”

“…Oral cancer is one of the most common cancers in the world, and oral squamous cell carcinoma (OSCC) accounts for more than 90% of all oral malignancies. 1 , 2 , 3 , 4 Accumulating studies have shown that there is a close relation between the epithelial‐mesenchymal transition (EMT) and cancer progression. 5 , 6 , 7 EMT is the conversion of cells from epithelial phenotype to mesenchymal phenotype.…”

SIRT7 suppresses the epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis by promoting SMAD4 deacetylation

“…Oral cancer is one of the most common life-threatening types of cancer worldwide, with oral squamous cell carcinoma (OSCC) being the predominant form, accounting for over 90% of all oral malignancies [ 1 – 4 ]. OSCC is associated with high morbidity and mortality [ 5 , 6 ].…”

Construction of differential expression plasmids of NGF to detect its influence on PC12 cell neuronal differentiation

“…The clinical data of the specimens were classified by patient age, tumor size and degree of differentiation, which in turn was classified according to the Nottingham system ( Table I ) ( 23 ). Immunohistochemistry was carried out according to the EnVision two-step method as previously described ( 24 ), and PBS was used as a negative control. PGP9.5 was used as a neuronal marker, and its positive expression level was scored according to the following positive criteria: Cells that contain brown and yellow granules in the cytoplasm were considered positive, and the total number of positive cells in 100 cells in every five fields of view was counted.…”