Immunohistochemical Analysis of Sialic Acid and Fucose Composition in Human Colorectal Adenocarcinoma

Fernández-Rodrı́guez, Julia; Feijoo-Carnero, Carmen; Merino-Trigo, Ana; Cadena, Marı́a Páez de la; Rodrı́guez-Berrocal, Francisco Javier; Carlos, Alejandro de; M, Butrón; Martínez-Zorzano, Vicenta S.

doi:10.1159/000030122

Cited by 34 publications

(15 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An increased fucose metabolism could be associated with malignancy, which seems to affect protein turnover [51,52]. Our results support this concept.…”

Section: Discussionsupporting

confidence: 83%

Impact of naringenin on glycoprotein levels in N-methyl-N′-nitro-N-nitrosoguanidine-induced gastric carcinogenesis in rats

et al. 2008

View full text Add to dashboard Cite

We have studied the chemopreventive role of naringenin against experimental gastric carcinogenesis in male Wistar rats. The animals were divided into five groups and six animals were included in each group. Stomach, liver, sera and kidney specimens were collected in the 20th week and the level of glycoproteins namely, hexose, hexosamine, sialic acid and fucose, were measured in the control, gastric cancer-induced, cancer naringenin pretreated, cancer naringenin posttreated and naringenin alone animals. The glycoprotein levels were increased in the gastric cancer-induced rats when compared with the control rats. The levels of glycoprotein were decreased significantly in cancer-bearing rats supplemented with naringenin as compared with the gastric cancer-induced rats. The result shows the gastroprotective effect of naringenin and describes the likelihood of naringenin in maintaining the integrity of cell membranes and gastric mucosa against oxidative damage. Moreover, we hypothesize that regulation of glycoprotein levels by naringenin could be associated with the regression of N-methyl-N'-nitro-N-nitrosoguanidine-induced gastric carcinoma.

show abstract

“…An increased fucose metabolism could be associated with malignancy, which seems to affect protein turnover [51,52]. Our results support this concept.…”

Section: Discussionsupporting

confidence: 83%

Impact of naringenin on glycoprotein levels in N-methyl-N′-nitro-N-nitrosoguanidine-induced gastric carcinogenesis in rats

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Lectins conjugated with DIG were used following the method previously described by Fernández-Rodríguez et al [33], but slightly modified. Briefly, sections were preincubated overnight in a moist chamber with blocking solution and rinsed twice in Tris buffer saline (TBS).…”

Section: Methodsmentioning

confidence: 99%

Ultrastructure and Glycoconjugate Pattern of the Foot Epithelium of the AbaloneHaliotis tuberculata(Linnaeus, 1758) (Gastropoda, Haliotidae)

Bravo

Martínez-Zorzano²,

Pérez³

et al. 2012

The Scientific World Journal

View full text Add to dashboard Cite

The foot epithelium of the gastropod Haliotis tuberculata is studied by light and electron microscopy in order to contribute to the understanding of the anatomy and functional morphology of the mollusks integument. Study of the external surface by scanning electron microscopy reveals that the side foot epithelium is characterized by a microvillus border with a very scant presence of small ciliary tufts, but the sole foot epithelium bears a dense field of long cilia. Ultrastructural examination by transmission electron microscopy of the side epithelial cells shows deeply pigmented cells with high electron-dense granular content which are not observed in the epithelial sole cells. Along the pedal epithelium, seven types of secretory cells are present; furthermore, two types of subepithelial glands are located just in the sole foot. The presence and composition of glycoconjugates in the secretory cells and subepithelial glands are analyzed by conventional and lectin histochemistry. Subepithelial glands contain mainly N-glycoproteins rich in fucose and mannose whereas secretory cells present mostly acidic sulphated glycoconjugates such as glycosaminoglycans and mucins, which are rich in galactose, N-acetyl-galactosamine, and N-acetyl-glucosamine. No sialic acid is present in the foot epithelium.

show abstract

“…Poly- N -acetyllactosamine formation is a crucial step for the terminal modification by Sia and Fuc (31). The carbohydrates that contain Sia or Fuc perform important roles in the various steps of tumor formation and tumor progression (35, 36). Enhanced expression of certain Sia types or their linkages is often correlated with the poor prognosis for many human malignancies.…”

Section: O-linked Glycan Structures and Their Changes In Cancer Cellsmentioning

confidence: 99%

Sweet and Sour: The Impact of Differential Glycosylation in Cancer Cells Undergoing Epithelialâ€“Mesenchymal Transition

Freire-de-Lima

2014

Front. Oncol.

View full text Add to dashboard Cite

Glycosylation changes are a feature of disease states. One clear example is cancer cells, which commonly express glycans at atypical levels or with different structural attributes than those found in normal cells. Epithelial–mesenchymal transition (EMT) was initially recognized as an important step for morphogenesis during embryonic development, and is now shown to be one of the key steps promoting tumor metastasis. Cancer cells undergoing EMT are characterized by significant changes in glycosylation of the extracellular matrix (ECM) components and cell-surface glycoconjugates. Current scientific methodology enables all hallmarks of EMT to be monitored in vitro and this experimental model has been extensively used in oncology research during the last 10 years. Several studies have shown that cell-surface carbohydrates attached to proteins through the amino acids, serine, or threonine (O-glycans), are involved in tumor progression and metastasis, however, the impact of O-glycans on EMT is poorly understood. Recent studies have demonstrated that transforming growth factor-beta (TGF-β), a known EMT inducer, has the ability to promote the up-regulation of a site-specific O-glycosylation in the IIICS domain of human oncofetal fibronectin, a major ECM component expressed by cancer cells and embryonic tissues. Armed with the knowledge that cell-surface glycoconjugates play a major role in the maintenance of cell homeostasis and that EMT is closely associated with glycosylation changes, we may benefit from understanding how unusual glycans can govern the molecular pathways associated with cancer progression. This review initially focuses on some well-known changes found in O-glycans expressed by cancer cells, and then discusses how these alterations may modulate the EMT process.

show abstract

Immunohistochemical Analysis of Sialic Acid and Fucose Composition in Human Colorectal Adenocarcinoma

Cited by 34 publications

References 27 publications

Impact of naringenin on glycoprotein levels in N-methyl-N′-nitro-N-nitrosoguanidine-induced gastric carcinogenesis in rats

Impact of naringenin on glycoprotein levels in N-methyl-N′-nitro-N-nitrosoguanidine-induced gastric carcinogenesis in rats

Ultrastructure and Glycoconjugate Pattern of the Foot Epithelium of the AbaloneHaliotis tuberculata(Linnaeus, 1758) (Gastropoda, Haliotidae)

Sweet and Sour: The Impact of Differential Glycosylation in Cancer Cells Undergoing Epithelialâ€“Mesenchymal Transition

Contact Info

Product

Resources

About