Ultrastructure and Glycoconjugate Pattern of the Foot Epithelium of the Abalone<i>Haliotis tuberculata</i>(Linnaeus, 1758) (Gastropoda, Haliotidae)

Bravo, Isabel; Martínez-Zorzano, Vicenta S.; Pérez, Iago Molist; García, Pilar M. Casares

doi:10.1100/2012/960159

Cited by 24 publications

(43 citation statements)

References 39 publications

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“…Transmission electron microscopy is a useful tool for examining the ultrastructure of biological materials (e.g., [11, 12]), which has been applied in plant cuticle anatomical studies [13]. The majority of these investigations were performed during the 1980s with few plant species and organs (e.g., [10, 14]).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy

Guzmán

Fernández

Khayet

et al. 2014

The Scientific World Journal

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The leaf cuticular ultrastructure of some plant species has been examined by transmission electron microscopy (TEM) in only few studies. Attending to the different cuticle layers and inner structure, plant cuticles have been grouped into six general morphological types. With the aim of critically examining the effect of cuticle isolation and preparation for TEM analysis on cuticular ultrastructure, adaxial leaf cuticles of blue-gum eucalypt, grey poplar, and European pear were assessed, following a membrane science approach. The embedding and staining protocols affected the ultrastructure of the cuticles analysed. The solubility parameter, surface tension, and contact angles with water of pure Spurr's and LR-White resins were within a similar range. Differences were however estimated for resin : solvent mixtures, since Spurr's resin is combined with acetone and LR-White resin is mixed with ethanol. Given the composite hydrophilic and lipophilic nature of plant cuticles, the particular TEM tissue embedding and staining procedures employed may affect sample ultrastructure and the interpretation of the results in physicochemical and biological terms. It is concluded that tissue preparation procedures may be optimised to facilitate the observation of the micro- and nanostructure of cuticular layers and components with different degrees of polarity and hydrophobicity.

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Section: Introductionmentioning

confidence: 99%

Ultrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy

Guzmán

Fernández

Khayet

et al. 2014

The Scientific World Journal

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“…The abalone foot muscle is known to be darker in color than the adductor due to the presence of pigments such as melanin, carotenoids, and bilichromes such as haliotisrubin (Voltzow ; Portela and others ). Consumers typically prefer lighter meat, and abalones with dark pigmentation may be heavily trimmed or bleached (Brown and others ).…”

Section: Resultsmentioning

confidence: 99%

Effects of Rigor Status during High‐Pressure Processing on the Physical Qualities of Farm‐Raised Abalone (Haliotis rufescens)

Hughes

Greenberg

Yang

et al. 2014

Journal of Food Science

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High-pressure processing (HPP) is used to increase meat safety and shelf-life, with conflicting quality effects depending on rigor status during HPP. In the seafood industry, HPP is used to shuck and pasteurize oysters, but its use on abalones has only been minimally evaluated and the effect of rigor status during HPP on abalone quality has not been reported. Farm-raised abalones (Haliotis rufescens) were divided into 12 HPP treatments and 1 unprocessed control treatment. Treatments were processed pre-rigor or post-rigor at 2 pressures (100 and 300 MPa) and 3 processing times (1, 3, and 5 min). The control was analyzed post-rigor. Uniform plugs were cut from adductor and foot meat for texture profile analysis, shear force, and color analysis. Subsamples were used for scanning electron microscopy of muscle ultrastructure. Texture profile analysis revealed that post-rigor processed abalone was significantly (P < 0.05) less firm and chewy than pre-rigor processed irrespective of muscle type, processing time, or pressure. L values increased with pressure to 68.9 at 300 MPa for pre-rigor processed foot, 73.8 for post-rigor processed foot, 90.9 for pre-rigor processed adductor, and 89.0 for post-rigor processed adductor. Scanning electron microscopy images showed fraying of collagen fibers in processed adductor, but did not show pressure-induced compaction of the foot myofibrils. Post-rigor processed abalone meat was more tender than pre-rigor processed meat, and post-rigor processed foot meat was lighter in color than pre-rigor processed foot meat, suggesting that waiting for rigor to resolve prior to processing abalones may improve consumer perceptions of quality and market value.

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“…Type C granules in the subepithelial regions of individuals of M. crenulata resemble type G granules of the abalone, especially in the maturing stages (Bravo Portela et al. ; fig. f).…”

Section: Discussionmentioning

confidence: 99%

“…Regarding marine invertebrates, the role of mucus in gastropods has received considerable attention with respect to its production and functions, reviewed by Davies & Hawkins (1998). To understand the seemingly contradictory functions of mucus as a lubricant as well as an adhesive, some morphological studies have begun to identify the types of secretory cells potentially contributing to the final mucous coating (Cranfield 1973;Bravo Portela et al 2012). Studies analyzing histological staining patterns at the light microscope level are useful yet limited due to the small size and difficulty in identifying the numerous types of secretory cells.…”

mentioning

confidence: 99%

Morphology and function of the skin epithelium covering the giant keyhole limpet Megathura crenulata

Martin

Bailey

Cohen

et al. 2018

Invertebrate Biology

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Mucus‐secreting epithelia protect marine gastropods from abrasive particles and microbes in seawater. We studied the morphology and function of the epithelium in the giant keyhole limpet Megathura crenulata. All exposed surfaces of the limpets were covered by a mucus‐secreting, simple columnar epithelium, in which most cells on the sole of the foot bore cilia, while the majority of cells on the side of the foot and three mantle regions bore microvilli. None of the coatings had antibacterial properties. The epithelium of the foot was distinct from that of other regions, in that the mucus secreted was used for locomotion and was left behind as the limpet moved. The glycocalyx bound to the microvilli of the mantle cells appeared to be clean in visual and SEM examinations, and attempts to enhance the binding of inert particles and bacteria were unsuccessful. Because studies have shown that standard tissue processing may cause artifactual shrinkage of mucous layers, cryostat sections and additives to standard fixatives were tested, but we found no change in the thickness of the mucous layers of the limpets compared to routinely processed tissues. Sloughing of the glycocalyx in vertebrate systems removes bound microbes, and alterations of the glycocalyx layers are associated with disease conditions. Sloughing of the glycocalyx on limpets was rarely observed. In one case, the outer mantle of the limpet was covered with silt and the glycocalyx appeared to be detaching. This process could be experimentally induced by dousing the outer mantle with talc particles. The types of secretory cells producing the mucus in each region of the skin were characterized using standard histological stains and lectin staining. Because analysis was hampered by their small size, classification of the types of secretory cells was based on TEM descriptions of granule morphology, which allowed for comparison to the secretory cells described from skin of the abalone. The possible roles of the numerous secretions, and the mechanisms of mucus production and loss, are compared to what is known in vertebrate systems and to our relative lack of knowledge regarding invertebrate systems.

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Ultrastructure and Glycoconjugate Pattern of the Foot Epithelium of the AbaloneHaliotis tuberculata(Linnaeus, 1758) (Gastropoda, Haliotidae)

Cited by 24 publications

References 39 publications

Ultrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy

Ultrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy

Effects of Rigor Status during High‐Pressure Processing on the Physical Qualities of Farm‐Raised Abalone (Haliotis rufescens)

Morphology and function of the skin epithelium covering the giant keyhole limpet Megathura crenulata

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