Immunoglobulins in Nasal Polyp Fluid

Donovan, R E; Johansson, S. G. O.; Bennich, H.; Soothill, J F

doi:10.1159/000230229

Cited by 100 publications

(55 citation statements)

References 5 publications

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“…The data for total albumin, IgG, and IgD were analyzed adapting the formula proposed by Donovan et al (8) The ratio of albumin in the secretion to the albumin in the serum is used as the correction factor. Since the molecular weight of albumin is lower than that of immunoglobulins, leakage from serum may be higher for the albumin.…”

Section: Resultsmentioning

confidence: 99%

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IgD in Human Colostrum

et al. 1985

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ABSTRACT. Simultaneous colostrum (C) and plasma samples (P) from 14 women, 1 to 5 days postpartum, were examined. Total IgD and specific IgD antibodies to & lactoglobulin, bovine serum albumin, Bermuda grass, and a-gliadin were measured by solid phase radioimmunoassay. The geometric mean concentrations of IgD were 35.8 (range 2.2-410) &dl for colostrum and 591.3 (range 72-4100) pgldl for plasma. Six subjects had a specific IgD antibody C/P ratio more than 10-fold greater than the total IgD C/P ratio, suggesting enhancement of antibody to a specific antigen in the mammary gland. All six had C/P ratios suggestive of local enhancement of IgD antibody to Bermuda grass, and two met this criterion for enhancement of IgD antibodies to &lactoglobulin, bovine serum albumin, or a-gliadin. Specimens for these studies were obtained during the peak grass pollen season. Seventeen additional subjects were studied to compare total IgD in colostrum and plasma with total IgG and serum albumin. The mean C/P ratio for IgD (0.055 f 0.015) exceeded the C/P ratio for total IgG (0.015 f 0.003) or total albumin (0.020 f 0.002). For 14 of 17 subjects the colostrum/plasma ratio for IgD exceeded the C/P ratio for albumin or IgG. Data were transformed logarithmically and correlation coefficients calculated. For albumin versus IgG in colostrum, there was a strong correlation, r = 0.865, p = 0.001. This was different from albumin versus IgD, r = 0.489, p = 0.046 and from IgD versus IgG, r = 0.556, p = 0.020. These analyses support a different mechanism of entry of IgD into milk compared to I& or albumin. These studies also suggest that IgD antibodies may participate in responses of the mucosal immune system. (Pediatr Res 19: 122-126,1985) Abbreviations C, colostrum P, plasma C/P, colostrum/plasma ratio BG, Bermuda grass BLG, j3-lactoglobulin BSA, bovine serum albumin Recent interest in both animal and human breast milk immunology concerns homing of lymphoblasts from mucosal sites to the mammary gland with resultant local production of im-

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Section: Resultsmentioning

confidence: 99%

“…When colostrum concentrations of IgD were corrected for the serum contributions adapting the formula of Donovan et al (8) for secretory antibody production, a strong enhancement of IgD was found in colostrum. This was not the case for IgG, providing additional support for possible local mammary production of IgD.…”

Section: Discussionmentioning

confidence: 99%

IgD in Human Colostrum

et al. 1985

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“…concentrations > 200 units/ml. (Tada and Ishizaka, 1970) (tonsil and adenoid tissue, bronchial and peritoneal lymph nodes, and respiratory and gastrointestinal mucosa), and IgE is present in a number of secretions including bronchial (Waldman, Virchow, and Rowe, 1973), middle ear (Ishikawa, Bernstein, Reisman, and Arbesman, 1972), lacrimal (Brauninger and Centifano, 1971), salivary (Ozkaragoz, Smith, and Gokcen, 1972), nasal (Donovan, Johansson, Bennich, and Soothill, 1970;H{ubday, Cake, and Turner, 1971), and urinary (Turner, Johansson, Barratt, and Bennich, 1970;Barratt, Turner, and Johansson, 1972;Stokes, Hosking, Turner, and Johansson, 1973 Urquhart andothers(1965), who have shown that, in rats infected with the nematode, Nippostrongylus brasiliensis, the formation of IgE-like antibodies in response to the infection leads to the successful control of the infection. It has been postulated that the rat, in order successfully to purge its gastrointestinal tract of the parasite, must form this IgE-like antibody.…”

Section: Methodsmentioning

confidence: 99%

Increased serum immunoglobulin E concentrations in venereal diseases.

Green¹,

Scales²,

Kraus³

1976

Sexually Transmitted Infections

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“…Depending upon the selection of healthy controls and the techniques used, mean values of between 36 and 71 units/ml (1 unit is about 2 ng) have been reported for adults [14], IgE also oc curs in tracheobronchial secretion [8] and sputum [12,32], in nasal secretion [10-12, 14, 19-22, 35] and polyp fluid [9] and in tears [5,17]. In contrast to IgA, the biochem ical and immunological properties of IgE in secretions and in serum are similar [3,21].…”

Section: Introductionmentioning

confidence: 99%

Immunoglobulins in Nasal Secretion with Special Reference to IgE

Johansson¹,

Deuschl²

1976

Int Arch Allergy Immunol

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Different ways of collecting relatively large volumes of nasal secretion with as physiological a composition as possible were studied. Nasal secretion was collected by the so-called nasal spray washing method from 5 patients with allergic rhinitis due to pollen and 5 healthy persons during a pollen-free season. The collection was performed without any provocation and following nasal provocation with histamine or allergen solution. With the radioimmunosorbent test, in which the lower limit of measurement was 0.1 units/ml, IgE could be quantified in 52 of 60 analysed secretions. IgA, IgG and albumin were demonstrated in all secretions. In the allergic patients, following histamine and allergen provocation, a relative increase in the concentration of IgE and albumin and a significant decrease of the IgA/albumin ratio in nasal secretion was found. In the healthy subjects, such changes in the secretion were observed only after histamine provocation. Calculations also suggested some local production of IgE, but not at all of the same order of magnitude as of IgA.

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Immunoglobulins in Nasal Polyp Fluid

Cited by 100 publications

References 5 publications

IgD in Human Colostrum

IgD in Human Colostrum

Increased serum immunoglobulin E concentrations in venereal diseases.

Immunoglobulins in Nasal Secretion with Special Reference to IgE

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