Immunoglobulins and transcription factors in adenoids of children with otitis media with effusion and chronic rhinosinusitis

Adenoidectomy With Balloon Catheter Sinuplasty: A Randomized Trial for Pediatric Rhinosinusitis

“…6,7 One study looked at patients with nasal discharge undergoing adenoidectomy and found no correlation between adenoid size and Lund-McKay score. 8 Studies of immune function have shown that, in children with CRS, there is a reduced expression of immunoglobulin A in the adenoid tissue 9 and higher levels of inflammatory cell activation markers and cytokines associated with tissue remodeling. 10 Due to the overlap with other inflammatory/infectious conditions, the clinical diagnosis of CRS in children is more challenging as compared to adults.…”

Differential characteristics of pediatric sinusitis in patients who underwent endoscopic sinus surgery: children vs. adolescents

“…First, we excluded patients who underwent tonsillectomy (with/without adenoidectomy). A previous study reported that immunoglobulin production in adenoids was associated with paediatric chronic rhinosinusitis; therefore, we wanted to avoid the potential influence of the absence of tonsil or adenoid on the analysis . Second, we could not evaluate the prevalence of allergic rhinitis or other atopic diseases in our patient population, because our study was a retrospective study, and data regarding allergic rhinitis was insufficient.…”

Differential expression of Immunoglobulin A in the adenoids of children with and without exudative otitis media

“…The slides were heated in a 60 8C oven, deparaffinized three times with xylene for 5 min each, dehydrated gradually with low concentrations of alcohol, and washed with distilled water. Sections were treated with 3% hydrogen peroxide for 10 min and washed with phosphate buffered saline (PBS) to suppress endogenous peroxidases [10]. Antibodies to IgA (Novocastra Laboratories Ltd, UK) were diluted 1:2000.…”

“…For each sample, the score was calculated for four different areas and averaged. The staining score was calculated by multiplying the percentage of stained cells by the staining intensity [10].…”

“…However, up till now, there has been no consensus the relationship between IgA and OME. Some reports have revealed that IgA staining in adenoid lymphocytes was significantly lower in children with OME compared to those without OME [10], and Hong et al reported that the staining of adenoidal areas stained for IgA was significantly higher in children with OME compared to those without OME [11]. In order to determine the relationship between adenoid IgA and OME, we conducted a study in children of different age groups with or without OME to evaluate changes in IgA levels in samples of adenoid tissue.…”