Immunoglobulin repertoire of B lymphocytes infiltrating breast medullary carcinoma

Kotlan, Beatrix; Simsa, Peter; Földi, J.; Wh, Fridman; Glassy, Mark C.; McKnight, Michael E.; Jl, Teillaud

doi:10.3233/hab-2003-12402

Cited by 24 publications

(12 citation statements)

References 28 publications

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“…Interestingly, a higher than 3 R/S ratio in CDR regions was found only in the selected overrepresented clusters. Our earlier and present results together with the recently published data about ductal breast carcinoma and MBC (22,24,35,38) suggest that at least part of the B cell repertoire of TIL-B cells is tumor-associated Ag driven (39 -41). The focus of our study was to obtain experimental proof of this Ag-driven response by identifying TIL-B-specific tumor-binder cancerrestricted clone(s).…”

Section: Discussionsupporting

confidence: 72%

“…To find homologous sequences, a databank search via National Center for Biotechnology Information Blast server to GenBank/European Molecular Biology Laboratory Net databases was conducted and the generated data termed as Blastn result. More details about data analysis will be published elsewere (35).…”

Section: Cloning and Sequencing Of V H -J H V-j And V-j Til-b Ig Rmentioning

confidence: 99%

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Novel Ganglioside Antigen Identified by B Cells in Human Medullary Breast Carcinomas: The Proof of Principle Concerning the Tumor-Infiltrating B Lymphocytes

Kotlan

Simsa²,

Teillaud

et al. 2005

The Journal of Immunology

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The potential tumor-recognizing capacity of B cells infiltrating human breast carcinoma is an important aspect of breast cancer biology. As an experimental system, we used human medullary breast carcinoma because of its heavy B lymphocytic infiltration paralleled to a relatively better prognosis. Ig-rearranged V region VH-JH, Vκ-Jκ, and Vλ-Jλ genes, amplified by RT-PCR of the infiltrating B cells, were cloned, sequenced, and subjected to a comparative DNA analysis. A combinatorial single-chain variable fragment Ab minilibrary was constructed out of randomly selected VH and Vκ clones and tested for binding activity. Our data analysis revealed that some of the VH-JH, Vκ-Jκ, and Vλ-Jλ region sequences were being assigned to clusters with oligoclonal predominance, while other characteristics of the Ab repertoire were defined also. A tumor-restricted binder clone could be selected out of the single-chain variable fragment κ minilibrary tested against membrane fractions of primary breast tumor cells and tumor cell lines, the VH of which proved to be the overexpressed VH3-1 cluster. The specific binding was confirmed by FACS analysis with primary breast carcinoma cells and MDA-MB 231 cell line. ELISA and thin layer chromatography dot-blot experiments showed this target Ag to be a ganglioside D3 (GD3). Our results are a proof of principle about the capacity of B cells infiltrating breast carcinomas to reveal key cancer-related Ags, such as the GD3. GD3-specific Abs may influence tumor cell progression and could be used for further development of diagnostic and/or therapeutic purposes.

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Section: Discussionsupporting

confidence: 72%

Section: Cloning and Sequencing Of V H -J H V-j And V-j Til-b Ig Rmentioning

confidence: 99%

Novel Ganglioside Antigen Identified by B Cells in Human Medullary Breast Carcinomas: The Proof of Principle Concerning the Tumor-Infiltrating B Lymphocytes

Kotlan

Simsa²,

Teillaud

et al. 2005

The Journal of Immunology

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show abstract

“…In this situation, their increase in size and number correlates with disease severity and local production of autoantibodies with an oligoclonal repertoire and hypersomatic mutations (Gause et al, 1995;Kotlan et al, 2003;RangelMoreno et al, 2006;Carragher et al, 2008;Humby et al, 2009). On the contrary, the development of tertiary lymphoid structures, which occurs in nonlymphoid organs in several infectious diseases, may be beneficial and help to resolve the infection.…”

Section: Immune Infiltration In Human Tumorsmentioning

confidence: 99%

Immune infiltration in human tumors: a prognostic factor that should not be ignored

et al. 2009

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“…Analysis of B cells from tumors and metastatic lymph nodes found hypermutated antibody genes and expansion of class-switched memory cells and plasmablasts in patients with bladder cancer [17]. Intratumoral germinal centers (GCs) have been reported to be present in NSCLC [18][19][20], breast cancer [21][22][23][24], colon cancer [25], and germ cell cancers [26]. In NSCLC, GCs are associated with early-stage disease [19], suggesting that a humoral immune response may hold these tumors in check.…”

mentioning

confidence: 99%

Interrogation of individual intratumoral B lymphocytes from lung cancer patients for molecular target discovery

Campa

Moody

Zhang

et al. 2016

Cancer Immunol Immunother

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Immunoglobulin repertoire of B lymphocytes infiltrating breast medullary carcinoma

Cited by 24 publications

References 28 publications

Novel Ganglioside Antigen Identified by B Cells in Human Medullary Breast Carcinomas: The Proof of Principle Concerning the Tumor-Infiltrating B Lymphocytes

Novel Ganglioside Antigen Identified by B Cells in Human Medullary Breast Carcinomas: The Proof of Principle Concerning the Tumor-Infiltrating B Lymphocytes

Immune infiltration in human tumors: a prognostic factor that should not be ignored

Interrogation of individual intratumoral B lymphocytes from lung cancer patients for molecular target discovery

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