Immunoglobulin G subclass values in healthy black and white children

Ambrosino, Donna M.; Black, C M; Plikaytis, Brian D.; Reimer, Charles B.; Lee, Mei C.; Evatt, Bruce L.; Carlone, George M.

doi:10.1016/s0022-3476(05)83036-7

Cited by 17 publications

(14 citation statements)

References 25 publications

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“…DiiTerence.s in relative proportions of males and females could account for some of the interstudy variations. No such sex difference was however detected by us and Ambrosino et al [14]. Criteria for including children as normal subjects were different in various studies.…”

Section: Discussionmentioning

confidence: 67%

“…In order to interpret IgG subclass concentrations in various liisordcrs [3|. many studies have reported IgG subclass normal ranges in children and adults [4][5][6][7][8][9][10][11][12][13][14]. and Beard et al [15] have made an interesting comparison of Ihc age-related mean and lower normal limits of IgG subclasses of six studies [6.7,9-!…”

Section: Discussionmentioning

confidence: 99%

“…The measurement of serum immunoglobulin G subclasses and its interpretation in paediatric age groups has been complicated by both the dilliculty in standardization of" methodologies and reagents [1][2][3] as well as fluctuations in concentrations of IgG subclasses with age [4][5][6][7][8][9][10][11][12][13][14]. The problem of standardization has been thoroughly reviewed and meaningful comparisons between laboratories are still not possible [1.3].…”

Section: Introductionmentioning

confidence: 99%

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Percentile ranges for serum IgG subclass concentrations in healthy Chinese children

Lau

Jones

et al. 1993

Clinical and Experimental Immunology

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SUMMARY[n order lo establish normal reference ranges of serum immunoglobulin G subclasses for different age groups in Chinese, we measured the four IgG subclasses by radial immunodiffusion using poiyclonal antisera in 350 normal healthy subjects (148 males and 202 females) recruited Irom ihe community. There was no signifieant sex difference for all the four IgG subclasses. Using Box-Cox transformation, we constructed smooth age-dependent percentile curves lor the four IgG subclasses. For the 50th percenliie values, the plateaus Tor igGl lo lgG4 are respectively 970 nig/dl at 13 years old. 481 mg/dl al 18. 48 mg/dl at 17 and 80 mg/dl at 13. Methodology, reagents, environmental and genetic factors might be responsible for the observed difference among the various reports. The IgG2 level in our Chinese population seemed to be higher than that in Caucasians, which might account for the very low incidence of invasive Haemophilus influenzae type b disease among the Chinese. Our IgGl level also plateaued later and at a higher level than that in other studies.

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Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Percentile ranges for serum IgG subclass concentrations in healthy Chinese children

Lau

Jones

et al. 1993

Clinical and Experimental Immunology

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“…In contrast to previous studies in Thai children (18,19), IgG4 concentrations were higher than those of IgG3 in most age groups between 4-10 years of age. Atopy, particularly eczema and food allergy (22), and nutritional status (23) are associated with increased IgG4 concentrations, but these were exclusion criteria in our study. The small number of subjects in the 12-15 years age group could account for the increased IgG4 concentrations seen.…”

Section: Discussionmentioning

confidence: 99%

Nephelometry determined serum immunoglobulin isotypes in healthy Thai children aged 2–15 years

Sitcharungsi

Ananworanich

Vilaiyuk

et al. 2012

Microbiology and Immunology

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Knowledge of what constitute normal serum immunoglobulin (Ig) concentrations are important for the diagnosis of immunologic disorders. Data on normal Ig evaluated by nephelometry are limited in healthy Asian children, none being available for Thai children. One hundred and forty-eight healthy Thai children aged 2-15 years were tested for serum immunoglobulins G, A, M, G1, G2, G3, and G4 (Ig G, A, M, G1, G2, G3, and G4) by nephelometry. Sixty-three percent were girls of median interquartile range age 6.9 (4.8-9.7) years. The geometric means for each Ig were summarized and categorized by age. Statistical analyses were used to compare Igs between sexes and age groups, and to compare IgG in this study with data from other published studies. The average ratios of IgG subclasses/IgG for Ig G1:2:3:4 were 66:22:5:7%. IgG, IgA, IgG2, and IgG3 concentrations showed a gradual increase with increasing age. There were no significant sex differences for any immunoglobulin isotype (P = 0.971). Our mean IgG concentration was lower than that measured by the radial diffusion method in healthy Thai children (P < 0.05). In all age groups, the mean IgG concentration in our study was significantly higher than that reported in Turkish and USA children, evaluated by the nephelometric and radial diffusion techniques, respectively (both P <0.001). This study provides information about normal Ig concentrations measured by nephelometry in healthy Asian children and illustrates the importance of ascertaining normal Ig values for age-and ethnic-matched controls using the same assay to diagnose immunologic disorders correctly.

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“…As Lee et al (16) showed, the reference limits of IgG subclasses vary between different populations and according to method. The variation according to ethnic group, even when using the same ELISA assay, was recently even more clearly demonstrated by Ambrosino et al (17). They found that healthy black children had lower levels of IgGi, IgG2 and IgG4.…”

Section: Discussionmentioning

confidence: 86%

Serum immunogiobulin G subclasses and serum immunogiobulin A in healthy Norwegian children and adults

Carisen

Mellbye²,

Fuglerud³

et al. 1992

Pediatric Allergy Immunology

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IgG subclass concentrations were determined by a capture ELISA antibody assay using monoclonal antibodies to IgG1, IgG2, IgG3, and IgG4. All the antibodies had been tested for specificity in an IUIS/WHO collaborative study, and this was confirmed by us by testing against purified myeloma proteins representing the 4 subclasses. The sera to be tested were diluted to obtain optimal sensitivity in the lower normal range for each subclass. With these serum dilutions, the lower limit of reading was 1. 2 g/l for IgG1, 0. 25 g/l for IgG2, 0.04 g/l for IgG3 and for IgG4. Age specific reference limits of the IgG subclass concentrations were determined in serum samples from 138 healthy infants and children under 14 years of age and 66 adults. The reference limits for each age group were determined by calculating the mean ± 2 SD of the logarithms to the values and then taking the antilog of the results. IgA was determined by a turbidimetric method with a reading limit of 0. 1 g/l, and the reference limits were calculated from serum samples from the 138 children under 14 years of age and from 31 healthy adults. The age specific reference limits of the IgG subclasses and IgA are given. Several infants and children had IgG4 levels below the lower reading limit. To determine lower reference limits of IgG4 below the age of 7 years was therefore of little clinical significance.

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Immunoglobulin G subclass values in healthy black and white children

Cited by 17 publications

References 25 publications

Percentile ranges for serum IgG subclass concentrations in healthy Chinese children

Percentile ranges for serum IgG subclass concentrations in healthy Chinese children

Nephelometry determined serum immunoglobulin isotypes in healthy Thai children aged 2–15 years

Serum immunogiobulin G subclasses and serum immunogiobulin A in healthy Norwegian children and adults

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