Immunogenicity of Sm32 synthetic peptides derived from the Schistosoma mansoni adult worm

Noya, Óscar; Noya, Belkisyolé Alarcón de; Guzmán, Fanny; Bermúdez, Henry

doi:10.1016/s0165-2478(03)00086-5

Cited by 10 publications

(5 citation statements)

References 22 publications

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“…The use of synthetic peptides composed of species-specific and conserved epitopes have a potential of minimising cross-reactivity whilst improving specificity and sensitivity [ 36 , 68 , 69 ]. Furthermore, in silico discovery of immunoreactive B cell epitopes may allow the design of species-specific epitopes for serological diagnosis.…”

Section: Resultsmentioning

confidence: 99%

Diagnostic performances of Schistosoma haematobium and Schistosoma mansoni recombinant proteins, peptides and chimeric proteins antibody based tests. Systematic scoping review

Vengesai

Muleya²,

Midzi³

et al. 2023

PLoS ONE

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Background Traditional diagnostic tests for schistosome infections are suboptimal, particularly when the parasite burden is low. In the present review we sought to identify recombinant proteins, peptides, and chimeric proteins with potential to be used as sensitive and specific diagnostic tools for schistosomiasis. Methods The review was guided by PRISMA-ScR guidelines, Arksey and O’Malley’s framework, and guidelines from the Joanna Briggs Institute. Five databases were searched: Cochrane library, PubMed, EMBASE, PsycInfo and CINAHL, alongside preprints. Identified literature were assessed by two reviewers for inclusion. A narrative summary was used to interpret the tabulated results. Results Diagnostic performances were reported as specificities, sensitivities, and AUC. The AUC for S. haematobium recombinant antigens ranged from 0.65 to 0.98, and 0.69 to 0.96 for urine IgG ELISA. S. mansoni recombinant antigens had sensitivities ranging from 65.3% to 100% and specificities ranging from 57.4% to 100%. Except for 4 peptides which had poor diagnostic performances, most peptides had sensitivities ranging from 67.71% to 96.15% and specificities ranging from 69.23% to 100%. S. mansoni chimeric protein was reported to have a sensitivity of 86.8% and a specificity of 94.2%. Conclusion The tetraspanin CD63 antigen had the best diagnostic performance for S. haematobium. The tetraspanin CD63 antigen Serum IgG POC-ICTs had a sensitivity of 89% and a specificity of 100%. Peptide Smp_150390.1 (216–230) serum based IgG ELISA had the best diagnostic performance for S. mansoni with a sensitivity of 96.15% and a specificity of 100%. Peptides were reported to demonstrate good to excellent diagnostic performances. S. mansoni multi-peptide chimeric protein further improved the diagnostic accuracy of synthetic peptides. Together with the advantages associated with urine sampling technique, we recommend development of multi-peptide chimeric proteins urine based point of care tools.

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Section: Resultsmentioning

confidence: 99%

Diagnostic performances of Schistosoma haematobium and Schistosoma mansoni recombinant proteins, peptides and chimeric proteins antibody based tests. Systematic scoping review

Vengesai

Muleya²,

Midzi³

et al. 2023

PLoS ONE

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“…The polypeptide of that antigen was recognized at 32KDa. Also, Noya et al (2003) recognized protein band at 32KDa from adult worm antigen by Western blot technique. Evengard et al (2008) reported that on immunoblots, using a freeze-dried adult worm antigen of S. mansoni, IgG1 and IgG3 antibodies were recognized by antigens at 32-35 kDa.…”

Section: Discussionmentioning

confidence: 99%

Comparative Study on Immunoblot versus PCR in Diagnosis of Schistosomiasis Mansoni in Experimental Infected Mice

Ismail

Mousa

Abu-Sarea

et al. 2016

JESP

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This study compared PCR and Western blot techniques in diagnosis of schistosomiasis mansoni. Forty Swiss albino mice were used, thirty two mice were infected with cercariae of S. mansoni and eight mice were kept uninfected which were used as a control. Blood was obtained from four infected mice weekly beginning from the 1 st week to the 8 th week post infection. The study found that PCR was positive from the first week post infection, while Western blot technique was positive from the second week post infection. Thus, PCR diagnosed schistosomiasis mansoni earlier than Western blot technique, but both were able to diagnose.

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“…It was shown to induce humoral response against the native protein and S. mansoni homogenate when used in DNA formulation but it failed to reduce the challenge worm burden (Chlichlia et al 2002). Additionally, hydrophilic regions of the molecule with Freund's adjuvant showed limited immunogenicity in rabbits and mice (Noya et al 2003a;Chacon et al 2003).…”

Section: Gastrointestinal Tract Vaccine Candidatesmentioning

confidence: 99%

A comprehensive and critical overview of schistosomiasis vaccine candidates

et al. 2021

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Immunogenicity of Sm32 synthetic peptides derived from the Schistosoma mansoni adult worm

Cited by 10 publications

References 22 publications

Diagnostic performances of Schistosoma haematobium and Schistosoma mansoni recombinant proteins, peptides and chimeric proteins antibody based tests. Systematic scoping review

Diagnostic performances of Schistosoma haematobium and Schistosoma mansoni recombinant proteins, peptides and chimeric proteins antibody based tests. Systematic scoping review

Comparative Study on Immunoblot versus PCR in Diagnosis of Schistosomiasis Mansoni in Experimental Infected Mice

A comprehensive and critical overview of schistosomiasis vaccine candidates

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