Immunogenicity of a scalable inactivated rotavirus vaccine in mice

Zhang, Biao; Yi, Shuhua; Ma, Yan; Zhang, Guangming; Zhang, Yongxin; Xie, Tian; Zhang, Dongliang; Sun, Maosheng

doi:10.4161/hv.7.2.14121

Cited by 11 publications

(7 citation statements)

References 24 publications

(35 reference statements)

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“…Moreover, lower production cost of inactivated rotavirus vaccine will also ensure greater accessibility and affordability to common people [92]. Several research groups are already trying to develop new technologies to produce an effective inactivated rotavirus vaccine [132,133]. Conventionally, rotavirus inactivation takes place by formalin, though it has some disputes [134,135].…”

Section: Inactivated Rotavirus Vaccine Best Alternative To Present Solution: Why and How?mentioning

confidence: 99%

Rotavirus epidemiology and vaccine demand: considering Bangladesh chapter through the book of global disease burden

Mahmud-Al-Rafat¹,

Muktadir²,

Muktadir³

et al. 2017

Infection

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Currently, two live attenuated vaccines are available throughout the world. Many countries have included rotavirus vaccines in national immunization program to reduce the disease burden. However, due to low efficacy of the available vaccines, satisfactory outcome has not yet been achieved in developing countries such as Bangladesh. Poor economic, public health, treatment, and sanitation status of the low-income countries necessitate the need for the most effective rotavirus vaccines. Therefore, the present scenario demands the development of a highly effective rotavirus vaccine. In this regard, inactivated rotavirus vaccine concept holds much promise for reducing the current disease burden. Recent advancements in developing an inactivated rotavirus vaccine indicate a significant progress towards disease prophylaxis and control.

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Section: Inactivated Rotavirus Vaccine Best Alternative To Present Solution: Why and How?mentioning

confidence: 99%

Rotavirus epidemiology and vaccine demand: considering Bangladesh chapter through the book of global disease burden

Mahmud-Al-Rafat¹,

Muktadir²,

Muktadir³

et al. 2017

Infection

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“…All samples were processed within 4 hours after collection. A micro-modified version of the original ELISPOT assay was used for simultaneous detection of magnetically enriched blood ASCs secreting IgA or IgG to RV (double layered rotavirus particles) [ 17 , 22 , 23 ]. Briefly, 1 ml of EDTA-treated blood was lysed with a red blood cell lysis solution for 5 minutes, washed with PBS-EDTA buffer following centrifugation, and re-suspended to the initial blood volume (1 ml) with PBS-EDTA buffer.…”

Section: Methodsmentioning

confidence: 99%

Antibody secreting B cells and plasma antibody response to rotavirus vaccination in infants from Kolkata India

Sinha

Kanungo

Kim

et al. 2018

Heliyon

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BackgroundAssessing immune response after rotavirus vaccination consists in measuring serum or plasma IgA and IgG antibodies, but these assays provide very little information about the mucosal immune response. Thus the development of assays for detection of mucosal immune response following rotavirus vaccination is essential. We evaluate to assess circulating antibody-secreting cells (ASCs) as a potential means to evaluate mucosal immune responses to rotavirus vaccine.Methods372 subjects, aged 6 weeks, were enrolled in the study. All the subjects were assigned to receive two doses of Rotarix® vaccine. Using a micro-modified whole blood-based ELISPOT assay, circulating rotavirus type-specific IgA- and IgG-ASCs, including gut homing β7+ ASCs, were enumerated on week 6 before the first dose of Rotarix vaccination at 7 weeks of age and week 18 after the second vaccination at 17 weeks of age. Plasma samples collected before vaccination, and after two doses of Rotarix® vaccination were tested for plasma rotavirus IgA titers.ResultsTwo doses of Rotarix® provided to induce sero-protective titer of ≥ 20 Units in 35% of subjects. Total blood IgA- ASC responses were detected in 26.4% of subjects who were non-responder before vaccination. Among responders, 47% of the subjects also have sero-protective plasma IgA titers.DiscussionOur results suggest that virus-specific blood gut homing ASCs were detected and provide insight into mucosal immune response after rotavirus vaccination. Further studies are needed to evaluate the duration of such immune responses and to assess the programmatic utility of this whole blood-based mucosal ASC testing for the rotavirus immunization program.

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“…Geometric mean titer was given. Microneutralization assay was performed to measure rotavirus neutralizing activity as described previously[ 20 ]. Serial diluted mouse serum was added to 96-well plates and incubated with 1000 PFU of Wa per well for 1 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of a new candidate human inactivated rotavirus vaccine strain from hospitalized children in Yunnan, China: 2010-2013

Zhou

Zhang

et al. 2018

WJCC

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AIMTo determine the distribution of rotavirus VP7 gene in hospitalized children in Yunnan, China.METHODSA total of 366 stool specimens were collected from hospitalized children in hospitals in Yunnan Province from September 2010 to December 2013. The genomic RNA electropherotypes and the G genotypes of the rotaviruses were determined. A phylogenetic analysis of the VP7 gene was performed. Rotavirus isolation was performed, and characterized by plaque, minimum essential medium, and all genes sequence analysis. Quantification of antibodies for inactivated vaccine prepared with ZTR-68 was examined by enzyme-linked immunosorbent assay and microneutralization assay.RESULTSGroup A human rotavirus was detected in 177 of 366 (48.4%) stool samples using a colloidal gold device assay. The temporal distribution of rotavirus cases showed significant correlation with the mean air temperature. Rotaviruses were isolated from 13% of the rotavirus-positive samples. The predominant genotype was G1 (43.5%), followed by G3 (21.7%), G9 (17.4%), G2 (4.3%), G4 (8.7%), and mixed (4.3%) among a total of 23 rotavirus isolates. A rotavirus strain was isolated from a rotavirus-positive stool sample of a 4-month-old child in The First People’s Hospital of Zhaotong (2010) for use as a candidate human inactivated rotavirus vaccine strain and for further research, and was designated ZTR-68. The genotype of 11 gene segments of strain ZTR-68 (RVA/Human-wt/CHN/ZTR-68/2010/G1P[8]) was characterized. The genotype constellation of strain ZTR-68 was identified as G1-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1. The VP7 and VP4 genotypes of strain ZTR-68 were similar to Wa-like strains.CONCLUSIONSA high prevalence of the G1, G2, and G3 genotypes was detected from 2010 to 2012. However, a dominant prevalence of the G9 genotype was identified as the cause of gastroenteritis in children in Yunnan, China, in 2013. A candidate human inactivated rotavirus vaccine strain, designated ZTR-68 was isolated, characterized, and showed immunogenicity. Our data will be useful for the future formulation and development of a vaccine in China.

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Immunogenicity of a scalable inactivated rotavirus vaccine in mice

Cited by 11 publications

References 24 publications

Rotavirus epidemiology and vaccine demand: considering Bangladesh chapter through the book of global disease burden

Rotavirus epidemiology and vaccine demand: considering Bangladesh chapter through the book of global disease burden

Antibody secreting B cells and plasma antibody response to rotavirus vaccination in infants from Kolkata India

Isolation and characterization of a new candidate human inactivated rotavirus vaccine strain from hospitalized children in Yunnan, China: 2010-2013

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