2018
DOI: 10.12998/wjcc.v6.i11.426
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Isolation and characterization of a new candidate human inactivated rotavirus vaccine strain from hospitalized children in Yunnan, China: 2010-2013

Abstract: AIMTo determine the distribution of rotavirus VP7 gene in hospitalized children in Yunnan, China.METHODSA total of 366 stool specimens were collected from hospitalized children in hospitals in Yunnan Province from September 2010 to December 2013. The genomic RNA electropherotypes and the G genotypes of the rotaviruses were determined. A phylogenetic analysis of the VP7 gene was performed. Rotavirus isolation was performed, and characterized by plaque, minimum essential medium, and all genes sequence analysis. … Show more

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Cited by 12 publications
(4 citation statements)
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“…A combined inactivated polio vaccine (IPV)–IRV product is also under development with this same strain, and has been tested in mice, with no evidence of interference of the immune response to either component [81]. Other early-stage candidates include an inactivated G1P[8] strain isolated from a hospitalized Chinese infant [82] and a truncated VP4 based on the LLR strain [83].…”
Section: Rotavirus Vaccine Pipelinementioning
confidence: 99%
“…A combined inactivated polio vaccine (IPV)–IRV product is also under development with this same strain, and has been tested in mice, with no evidence of interference of the immune response to either component [81]. Other early-stage candidates include an inactivated G1P[8] strain isolated from a hospitalized Chinese infant [82] and a truncated VP4 based on the LLR strain [83].…”
Section: Rotavirus Vaccine Pipelinementioning
confidence: 99%
“…The infectivity of the harvested virus solution was determined using Rotavirus antigen detection kit (colloidal gold method) (WANTAI Biopharm, Beijing,China). 32 Cell culture media from infected cells were filtered to remove cell debris, and clarified supernatants were concentrated through ultrafiltration membrane cassettes (Sigma-Aldrich, MO, USA). Concentrated live rotaviruses were purified further by ion exchange chromatography and gel filtration (General Electric Company, USA) purification.…”
Section: Methodsmentioning
confidence: 99%
“…Inactivated RV vaccines (IRVV) are origin from weakened or killed RV strains like human RV strain CDC-9, G2P [8], G3P [8], G9P [8] and G1P [8] and ZTR-68 by stimulating antibodies against RV infection. [170][171][172][173][174][175][176][177][178] IRVV protected animal models through different challenging routes like oral or intramuscular, microneedle delivery for skin vaccination in preclinical trials. According to several studies, intramuscular inoculation with three doses of IRVV could elicit high serum IgG antibody and neutralizing antibody responses in gnotobiotic piglets, 171 which is similar to the results of mice model.…”
Section: Other Candidate Vaccinesmentioning
confidence: 99%