Immunogenicity and protective efficacy of 3A truncated negative marker foot-and-mouth disease virus serotype A vaccine

Dhanesh, V.V.; Hosamani, M.; Basagoudanavar, Suresh H.; Saravanan, P.; Biswal, Jitendra K.; Selvan, R.P. Tamil; Madhavan, Aparna; Sehrish, Karishma; Sanyal, Aniket; Sreenivasa, B.P.

doi:10.1007/s00253-020-10370-z

Cited by 4 publications

(5 citation statements)

References 36 publications

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“…It was previously shown that FMDV serotype O IND-R2/1975 with 93–143 amino acids deletion in 3A and 10–37 amino acids deletion in 3B was rescuable (Biswal et al 2015 ). Also, in serotype A IND40/2000, we had rescued virus with amino acid residues 87–144 deletions in 3A region (Dhanesh et al 2020 ). Also, antibodies against the 3A and 3B are relatively more sensitive indicators of FMDV replication (Grubman 2005 ; Henderson 2005 ).…”

Section: Discussionmentioning

confidence: 99%

“…The antigen complex was detected by horseradish peroxidase (HRP)–conjugated rabbit anti-guinea pig immunoglobulin (Dako, Denmark, # P0141). Further, the antigenic relationship of recombinant viruses in comparison with parent wild-type virus was assessed using a two-dimensional microneutralization test, by determining r1-value as described previously (Dhanesh et al 2020 ). Briefly, the r1-value of recombinant viruses in relation with the currently used vaccine strain O/IND-R2/1975 was calculated by using bovine vaccinate serum (BVS) raised against serotype O FMDV (Rweyemamu et al 1978 ).…”

Section: Methodsmentioning

confidence: 99%

“…The plasmid pET32a t3A 87–144 containing deletion region (87–144 amino acids) of 3A protein of FMDV A-IND40/00 in the modified pET-32a( +) vector (Merck, USA, #69015) without thioredoxin tag was prepared previously (Dhanesh et al 2020 ). The plasmid was transformed into BL21(DE3)pLysS competent E. coli cells (Merck, USA, #69451), and positive colonies were selected by using chloramphenicol (34 µg/mL) and ampicillin (50 µg/mL).…”

Section: Methodsmentioning

confidence: 99%

“…The deletion of G–H loop in VP1 (Fowler et al 2014 ), leader coding region (Lpro), and 3B1/3D proteins have been evaluated for DIVA compliance (Uddowla et al 2012 ). It was shown that carboxyl-terminal of 3A region, 3B1, and 3B2 can accommodate deletions without compromising the replication efficiency of the virus (Pacheco et al 2003 ; Li et al 2014 ; Biswal et al 2015 ; Behura et al 2016 ; Bhatt et al 2018 ; Dhanesh et al 2020 ; Lalzampuia et al 2022 ). In this study, with the aim of producing FMDV vaccine with negative marker attribute and containing thermostable capsid, we generated a deletion mutant in 3A C-terminal region of O IND/R2/1975 virus with thermostable capsid having Y2098F mutation.…”

Section: Introductionmentioning

confidence: 99%

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Thermostable negative-marker foot-and-mouth disease virus serotype O induces protective immunity in guinea pigs

Ranjitha

Dhanesh

Hosamani

et al. 2023

Appl Microbiol Biotechnol

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Foot-and-mouth disease (FMD) is a contagious viral disease of high economic importance, caused by FMD virus (FMDV), a positive-sense single-stranded RNA virus, affecting cloven-hoofed animals. Preventive vaccination using inactivated virus is in practice to control the disease in many endemic countries. While the vaccination induces antibodies mainly to structural proteins, the presence of antibodies to the non-structural proteins (NSP) is suggestive of infection, a criterion for differentiation of infected from vaccinated animals (DIVA). Also, there is a growing demand for enhancing the stability of the FMD vaccine virus capsid antigen as the strength of the immune response is proportional to the amount of intact 146S particles in the vaccine. Considering the need for a DIVA compliant stable vaccine, here we report generation and rescue of a thermostable and negative marker virus FMDV serotype O (IND/R2/1975) containing a partial deletion in non-structural protein 3A, generated by reverse genetics approach. Immunization of guinea pigs with the inactivated thermostable-negative marker virus antigen induced 91% protective immune response. Additionally, a companion competitive ELISA (cELISA) targeting the deleted 3A region was developed, which showed 92.3% sensitivity and 97% specificity, at cut-off value of 36% percent inhibition. The novel thermostable-negative marker FMDV serotype O vaccine strain and the companion cELISA could be useful in FMDV serotype O enzootic countries to benefit the FMD control program. Key points • Thermostable foot-and-mouth disease virus serotype O with partial deletion in 3A . • Inactivated thermostable marker vaccine induced 91% protection in guinea pigs . • Companion cELISA based on deleted region in 3A could potentially facilitate DIVA . Supplementary information The online version contains supplementary material available at 10.1007/s00253-023-12359-w.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Thermostable negative-marker foot-and-mouth disease virus serotype O induces protective immunity in guinea pigs

Ranjitha

Dhanesh

Hosamani

et al. 2023

Appl Microbiol Biotechnol

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“…Given that FMDV has a worldwide spread and seriously affects global economy and trade, FMD is listed as a class A infectious disease by the World Organization for Animal Health (OIE). Therefore, the aforementioned studies provide a solid theoretical foundation for FMDV vaccine development and technical guidance for FMD control ( Aghaei et al, 2019 ; Dhanesh et al, 2020 ).…”

Section: Concluding Remarks and Further Perspectivesmentioning

confidence: 99%

Advances in Foot-and-Mouth Disease Virus Proteins Regulating Host Innate Immunity

Peng

Yang

et al. 2020

Front. Microbiol.

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Foot-and-mouth disease (FMD) is a highly contagious disease that affects cloven-hoofed animals such as pigs, cattle, and sheep. The disease is caused by the foot-and-mouth disease virus (FMDV) which has a non-enveloped virion with icosahedral symmetry that encapsulates a positive-sense, single-stranded RNA genome of ∼8.4 kb. FMDV infection causes obvious immunosuppressive effects on the host. In recent years, studies on the immunosuppressive mechanism of FMDV have become a popular topic. In addition, studies have shown that many FMDV proteins are involved in the regulation of host innate immunity and have revealed mechanisms by which FMDV proteins mediate host innate immunity. In this review, advances in studies on the mechanisms of interaction between FMDV proteins and host innate immunity are summarized to provide a comprehensive understanding of FMDV pathogenesis and the theoretical basis for FMD prevention and control.

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A new blocking ELISA for detection of foot-and-mouth disease non-structural protein (NSP) antibodies in a broad host range

Hosamani

Gopinath

Sreenivasa

et al. 2022

Appl Microbiol Biotechnol

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Immunogenicity and protective efficacy of 3A truncated negative marker foot-and-mouth disease virus serotype A vaccine

Cited by 4 publications

References 36 publications

Thermostable negative-marker foot-and-mouth disease virus serotype O induces protective immunity in guinea pigs

Thermostable negative-marker foot-and-mouth disease virus serotype O induces protective immunity in guinea pigs

Advances in Foot-and-Mouth Disease Virus Proteins Regulating Host Innate Immunity

A new blocking ELISA for detection of foot-and-mouth disease non-structural protein (NSP) antibodies in a broad host range

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