Immunogenicity and Antigenicity of Lymphoid Cells treated with Neuraminidase

Unmasking of Xenogeneic Neo-antigens on Mouse Lymphoid Cell Surfaces by Vibrio cholerae Neuraminidase

“…The question remains whether the serologic behavior of VCN treated cells helps explain the increased immunogenici ty of such cells in vivo (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)37,38). Increased immunogenicity of embryonic ( lo), lymphoid (9), and tumor cells (1)(2)(3)(4)(5)(6)(7)(8)37, 38) appears to be specific for antigens preexisting on the cell surface.…”

“…No differences in the two products could be detected in the previous (7)(8)(9)(10)(11)(12)(13)(14) experiments, so the former material was used throughout this study. The VCN was diluted with M199 (pH 7.2).…”

“…The latter effect is totally immunospecific and regression cannot be induced with an immunologically distinct tumor. VCN treated mouse lymphoid cells (9) and embryonic tissues (10) have also been found to be more immunogenic in allogeneic recipients. In vitro studies have further demonstrated that VCN treated stimulatory cells will elicit a much greater degree of transformation from allogeneic responder cells in mixed lymphocyte culture ( 1 1).…”

Glycosidases—Properties and Application to the Study of Complex Carbohydrates and Cell Surfaces

“…Normal cells also are rendered more immunogenic by the action of sialidase (244,(260)(261)(262)(263), and the immunogenicity and antigenicity of sheep, horse, and goat erythrocytes (264), are enhanced following incubation with V. cholerue sialidase. In the case of chicken erythrocytes, however, enzymatic removal of sialic acid was not accompanied with increase in immunogenicity (see ref.…”

Neuraminidase and Anti-Neuraminidase Serum: Effect on the Cell Surface Properties

“…The heterosaccharide components at the periphery of cells dic-tate many of the immunogenic and antigenic (receptor) characters of the intact cell [23]. The cellular immunogenicity, which may increase non-specifically [24][25][26], is not only confined to tumours but has been described in relation to embryonic tissue and normal lymphoid cells [6, 25-27], There are several other important consequences of RDE treatment of cells, including inhibi tion of viral [28] or mycoplasma [29] induced haemagglutination, increased cell aggregation, increased cell deformability [30], alterations in patterns of cellular migration [31], and interference with amino acid transport across RDE-treated cell membranes [32,50].…”