Unmasking of Xenogeneic Neo-antigens on Mouse Lymphoid Cell Surfaces by Vibrio cholerae Neuraminidase

Simmons, Richard L.

doi:10.3181/00379727-142-36992

Cited by 11 publications

(1 citation statement)

References 12 publications

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“…Target cells were incubated with 10 md. The same batch of G P serum was used as in Table V species of mouse lymphoid cells and that the cytotoxicity can be either enhanced, or extended to otherwise insensitive cells, after pre-treatment of the target cells with neuraminidase (Schlesinger and Amos, 1971;Ray et al, 1972;Ray and Simmons, 1973). It was postulated that this cytotoxicity could be due to cytotoxic antibodies directed against surface xenogeneic antigens of the mouse cells.…”

Section: Discussionmentioning

confidence: 99%

Sensitivity to guinea‐pig serum of hamster cells transformed in vitro by two strains of the rous sarcoma virus

Bataillon¹

1974

Intl Journal of Cancer

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BHK 21/13 hamster cells transformed in vitro by the Bryan (RB 12) and the Schmidt-Ruppin (RS2) strains of Rous sarcoma virus differ in their behaviour in tissue culture (Montagnier et Vigier, 1967). Whereas RS 2 cells have retained the spindle shape of the parental BHK 21/13 cells and show some contact inhibition of division in monolayers, RB 12 cells, on the contrary, are large rounded cells which rapidly pile up. RB 12 cells also have a higher cloning eficiency than R S 2 cells in liquid medium or soft agarose, and they are less dependent on environmental factors, such as composition of media and presence of feeder cells.The two kinds of cells differ also in some surface properties, and RS 2 cells were recently found to be more sensitive than RB 12 cells to the cytotoxic action of antisera directed against the RSV-induced surface antigen (VISA) (Bataillon, 19736).Comparative sensitivity of untransformed BHK 21/13, RB 12 and RS 2 cells to normal guinea-pig (GP) serum is presented in this paper.Whereas BHK 21-13 cells were highly sensitive to the cytotoxic action of GP serum, cells transformed by RSV had a reduced sensitivity (RS 2) or were entirely insensitive (RB 12). Mild treatment of the cells with Vibrio cholerae neuraminidase, which did not affect the sensitivity of BHK 21/13 and RS 2cells, rendered the RB 12 cells significantly sensitive to the cytotoxic action of normal GP serum. MATERIAL AND METHODS C e hRB 12/1, RB 1214, RS 213, RS 2/10 and RS 216 are five subclones derived from BHK21/13 hamster cells (Macpherson and Stoker, 1962) transformed in vitro with B-RSV [RAV-I] (clone RB 12) and SR-RSV (clone RS 2) respectively (Montagnier et Vigier, 1967;Vigier, 1967). C 13/15 and C 13/8, two subclones of BHK 21/13 isolated by Dr. L. Montagnier, and some properties of C 1318 cells, which are asparagine-dependent, have already been described .

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Section: Discussionmentioning

confidence: 99%

Sensitivity to guinea‐pig serum of hamster cells transformed in vitro by two strains of the rous sarcoma virus

Bataillon¹

1974

Intl Journal of Cancer

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Cell surface modification in the treatment of experimental cancer: Neuraminidase or concanavalin a

Simmons

Rios

1974

Cancer

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Firmly established tumors in syngeneic mice will regress if the host is challenged with living tumor cells which have been exposed to Vibrio cholerae neuraminidase (VCN) or Concanavalin A (ConA) in vitro. The effect is totally immunospecific and can be induced only with VCN‐ or ConA‐treated tumor cells identical in type with the growing tumor. The degree of immunoregression is similar when using optimal concentrations of ConA and VCN. The fact that two such different substances can alter the cell surface to increase the immunogenicity of tumor antigens may shed some light on the mechanisms involved.

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Antibodies against heterologous connectivetissue cells in rheumatic fever

Данилова¹,

Fedorova²

1974

Bull Exp Biol Med

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Unmasking of Xenogeneic Neo-antigens on Mouse Lymphoid Cell Surfaces by Vibrio cholerae Neuraminidase

Cited by 11 publications

References 12 publications

Sensitivity to guinea‐pig serum of hamster cells transformed in vitro by two strains of the rous sarcoma virus

Sensitivity to guinea‐pig serum of hamster cells transformed in vitro by two strains of the rous sarcoma virus

Cell surface modification in the treatment of experimental cancer: Neuraminidase or concanavalin a

Antibodies against heterologous connectivetissue cells in rheumatic fever

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