Immunogenic Reaction of Implanted Biomaterials from Nature

Griensven, Martijn van; Balmayor, Elizabeth R.

doi:10.1002/9781119126218.ch23

Cited by 2 publications

(1 citation statement)

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“…Additionally, the UCNPs doped with Tm/Er rare earth elements can upconvert the NIR to UV (365 nm) and visible light (475/540/650), which has broad application prospects in biomedicine . Moreover, mesoporous silica has also attracted interest in biomedical research because of its large specific surface area, high loading capacity of mesoporous structure, good biocompatibility, and other excellent drug delivery performances. − Mesoporous silicon-coated UCNPs have broad application prospects in biomedicine and have been broadly applied as optical probes and drug microcarriers in the treatment and testing of cancer and other diseases. − UCNPs have also been used as nanoplatforms or nanoprobes to control and monitor the differentiation of MSCs for tissue regeneration. ,, …”

Section: Introductionmentioning

confidence: 99%

Near-Infrared Light-Controlled and Real-Time Detection of Osteogenic Differentiation in Mesenchymal Stem Cells by Upconversion Nanoparticles for Osteoporosis Therapy

et al. 2022

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Osteoporosis (OP) is one of the most common diseases in the elderly, and it is not effectively solved by current treatments. Mesenchymal stem cells (MSCs) have multiple differentiation potentials, which can induce osteogenic differentiation to treat OP; however, it is important to understand how to remotely control and detect osteogenic differentiation in vivo in real time. Here, we developed an upconversion nanoparticle (UCNP)-based photoresponsive nanoplatform for near-infrared (NIR) light-mediated control of intracellular icariin (ICA) release to regulate the osteogenic differentiation of MSCs for OP therapy. We simultaneously detected osteogenic differentiation in vivo in real time to evaluate the treatment effects. The Tm/Er-doped UCNPs were synthesized and coated with mesoporous silica (UCNP@mSiO2) first. Then, the photocaged linker 4-(hydroxymethyl)-3-nitrobenzoic acid (ONA) and the PEG linker (OH-PEG4-MAL) were linked to the surface of UCNP@mSiO2 to conjugate to the cap β-cyclodextrin (β-CD) and the Arg-Gly-Asp (RGD)-targeted peptide/matrix metalloproteinase 13 (MMP13)-sensitive peptide-BHQ (CGPLGVRGK-BHQ3) to form the UCNP nanoplatform (UCNP@mSiO2-peptide-BHQ-ONA-CD) for drug loading. Under 980 nm NIR light, the upconverted UV from the UCNPs triggered the cleavage of cap β-CD and the intracellular release of ICA to induce the osteogenic differentiation of MSCs for OP therapy. Meanwhile, MMP13, which was produced by osteogenic differentiation of MSCs, cleaved the MMP13-sensitive peptide to remove BHQ and recover the fluorescence of UCNPs, allowing real-time detection of osteogenic differentiation and the evaluation of the OP treatment effect. This photoresponsive UCNP nanoplatform has the potential to be used for the remote control and real-time detection of osteogenic differentiation of MSCs for OP therapy by NIR.

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