Immunofluorescent study of alpha‐foetoprotein (αfp) in liver and liver tumours. I. Technique of αfp localization in tissue sections

Localization of T and B cells and alpha fetoprotein in hepatic biopsies from patients with liver disease.

“…Positive immunofluorescence was localized to clusters of hepatocytes, and sometimes single cells, many of which appeared to be near areas of liver cell regeneration. These findings are quite similar to those previously reported in the liver of patients with hepatoma by other workers (45,46). Our findings appear to be the first clear documentation of cellular localization of AFP in the liver of patients with alcoholic hepatitis.…”

Tissue specificity of alpha-fetoprotein messenger RNA expression during mouse embryogenesis.

“…In some experiments, RNA-DNA hybridization was quantified by cutting the hybrid dots from the nitrocellulose filter, drying under a heat-lamp, and counting bound radioactivity in toluene-based scintillation fluid. In situ hybridization Tissues from 14th day embryos were fixed by the Sainte-Marie technique with Engelhardt's modification (Engelhardt et al, 1971;Dziadek and Adamson, 1978), and subsequently embedded in paraffin wax. 8 pm sections were mounted onto gelatinized microscope slides, dried, de-paraffinized in toluene, and hydrated to 3 x SSC before hybridization.…”

Alpha-feto-protein during development and in disease.

“…Site of synthesis of AFP at cellular level In order to localize the site of production of AFP single fetal cells have been studied using immunofluorescence (Engelhardt et al, 1969;Abelev, 1971;Engelhardt et al, 1971). With this technique AFP was detected almost solely in the liver parenchyma.…”

“…When this non-specific effect was excluded it was found that only a small proportion of cancer liver cells shows evidence of synthesizing AFP. The AFP 'positive' cells were detected around capillaries of the tumour sinuses and did not exceed 15% of the total (Goussev et al, 1970;Engelhardt et al, 1971;Abelev, 1971;Masseyeff, 1972).…”