Immunofluorescent Studies in Adult Celiac Disease*

Rubin, Walter; Stephen, Anthony; Sleisenger, Marvin H.; Jeffries, Graham H.

doi:10.1172/jci105161

Cited by 139 publications

(32 citation statements)

References 28 publications

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“…In fact, gluten protein is subjected to nearly complete proteolytic degradation in vivo, whereas proteolytic degradation in vitro (used to (17) indicated that gluten binds to the epithelial cell of patients with GSE. We suggest that the various intestinal immune reactions occur as a consequence of gluten binding to the epithelial cell; i.e., unbound gluten is not immunogenic, whereas bound gluten behaves as a hapten.…”

Section: Discussionmentioning

confidence: 99%

Gluten-sensitive Enteropathy

Falchuk¹,

Nelson²,

Katz³

et al. 1980

J. Clin. Invest.

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A B S T R A C T We previously developed an in vitro organ culture system in which gluten exerts a toxic effect on intestinal mucosa of patients with active gluten-sensitive enteropathy. Gluten generally inhibits the epithelial cell maturation of intestinal biopsy specimens that otherwise occurs if the tissue is cultured for 24-48 h in a gluten-free medium. However, small intestinal mucosa from 15-20% of patients with proven gluten-sensitive enteropathy fails to manifest the expected gluten-induced damage in vitro. In the present study, we explored the relation between in vitro gluteninduced intestinal damage and the presence of HLA-B8.We determined whether the patients' histocompatibility type (HLA-B8 positive or negative) influenced the ability of gluten protein to inhibit epithelial cell maturation of cultured intestinal biopsy specimens from patients with gluten-sensitive enteropathy.Intestinal biopsies from 21 of 24 patients with gluten-sensitive enteropathy and HLA-B8 showed gluteninduced dacmage in vitro. On the other hand, intestinal biopsies from only 4 of 16 patients with gluten-sensitive enteropathy I)ut without HLA-B8 showed gluteninduced daimage in vitro. The difference in the effect of gluten in vitro between these two groups was statistically significant (P < 0.01). The data show a dichotomy between gluten-induced tissue damage in vivo and in vitro in HLA-B8 negative patients, suggesting that Presented in part at

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Section: Discussionmentioning

confidence: 99%

Gluten-sensitive Enteropathy

Falchuk¹,

Nelson²,

Katz³

et al. 1980

J. Clin. Invest.

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“…Fluorescein conjugates of each antiserum were prepared by a method that has been previously described (18).…”

Section: Methodsmentioning

confidence: 99%

Studies of parietal cell antibody in pernicious anemia.

Jeffries¹,

Sleisenger²

1965

J. Clin. Invest.

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“…Although there is no lack of compelling indirect evidence for the synthesis of "coproantibodies" (1,2) by the intestinal lymphoid tissue in response to local antigenic stimulation, direct proof of such activity in the plasma cells of the lamina propria of the gut still remains to be furnished. A renewal of interest in this field has resulted from the discovery by Tomasi and Zigelbanm (9) of IgA as the major immunoglobulin in human external secretions, as well as from the demonstration that plasma cells of the IgA class far outnumber all other types of immunoglobulin-synthesizing cells in the lamina propria (10,11).…”

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confidence: 99%

ANTIBODIES OF THE IgA TYPE IN INTESTINAL PLASMA CELLS OF GERMFREE MICE AFTER ORAL OR PARENTERAL IMMUNIZATION WITH FERRITIN

Crabbé

Nash

Bazin

et al. 1969

The Journal of Experimental Medicine

190

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In adult germfree C3H mice immunized with horse spleen ferritin, either subcutaneously or intraperitoneally, plasma cells containing specific antibodies were found in lymph nodes and spleen and, in smaller numbers, also in the lamina propria of the intestine. In extraintestinal sites, these antiferritin-containing plasma cells were mainly of the IgM class after a single stimulation, and of the IgG1 class after repeated stimulation. In the intestine, all the anti-ferritin-containing cells appeared to be of the IgA class. Circulating antibodies, after repeated stimulation, were for the major part IgG1 and IgG2. In germfree mice given ferritin in their drinking water, antiferritin-containing cells were abundant in the intestinal mucosa, much less numerous in the mesenteric lymph nodes, and extremely scarce in other lymphoid tissues. All these cells, whatever their location, appeared to belong exclusively to the IgA class. Similarly, all the circulating antibody in these animals was found to be IgA. These findings illustrate the role of the gut as a site of antibody synthesis, as well as its selective commitment to the production of antibodies of the IgA class.

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Immunofluorescent Studies in Adult Celiac Disease*

Cited by 139 publications

References 28 publications

Gluten-sensitive Enteropathy

Gluten-sensitive Enteropathy

Studies of parietal cell antibody in pernicious anemia.

ANTIBODIES OF THE IgA TYPE IN INTESTINAL PLASMA CELLS OF GERMFREE MICE AFTER ORAL OR PARENTERAL IMMUNIZATION WITH FERRITIN

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