Immunofluorescent localization of collagen types I and III, and of fibronectin during feather morphogenesis in the chick embryo

Mauger, Annick; Démarchez, Michel; Herbage, D.; Grimaud, Jean‐Alexis; Druguet, M.; Hartmann, Daniel; Sengel, P

doi:10.1016/0012-1606(82)90072-0

Cited by 74 publications

(39 citation statements)

References 21 publications

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“…Coincident with the formation of the dermis there is a redistribution of the Type I and I11 collagens from their location at the epidermal-dermal junction (EDJ) to the newly developing dermis (Mauger et al, 1982). The present study demonstrates that the cells of the dermis differ from the surrounding mesenchymal cells by their lack of pl-integrin immunostaining.…”

Section: Discussionmentioning

confidence: 47%

“…In normal backskin the dermal condensations of feather germs are not labeled with the pl-integrin antiserum. This produces a heterogeneous immunostaining pattern very similar to the pattern seen for Type I collagen (Mauger et al [1982] Dev. Biol.…”

Section: Introductionmentioning

confidence: 65%

“…This is the first evidence demonstrating that the mesenchymal cell population in this central region is different from the populations in the lateral regions. Other molecules such as collagen, fibronectin, proteoglycan, and laminin are distributed more uniformly in the dorsal region a t this early stage of development (Mauger et al, 1982Kitamura, 1987).…”

Section: Discussionmentioning

confidence: 99%

“…Stabilization of the feather pattern requires that spatial barriers between the feather germs be set up to maintain the morphological integrity of individual feather germs (Mauger et al, 1982). The intergerm regions are considered to be the spatial barriers that separate and stabilize individual feather germs.…”

Section: Discussionmentioning

confidence: 99%

“…9E; Mauger et al, 1982). However, the pl-integrin immunostaining pattern is visible at least 1 day before the appearance of the Type I collagen pattern.…”

Section: -71h Days Of Incubation (Hh Stages 28-32)mentioning

confidence: 96%

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Pattern formation in chick feather development: Distribution of β1‐integrin in normal and scaleless embryos

Song

Carver

Sawyer

1994

Developmental Dynamics

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We have examined the immunolocalization of pl-integrin during feather development in the spino-lumbar tract of the backskin from normal and scaleless chick embryos. pl-integrin appears during early feather development in three distinct phases which correspond to important developmental events. The first phase (5-5% days of incubation; Hamburger and Hamilton [H.H.] stage 27) represents the period prior to the formation of dermis. During this phase, pl-integrin antiserum labels mesenchymal cells located in the central region of the spino-lumbar tract where the initiation site for feather development is located. The second phase (51/2-71/2 days of incubation; H.H. stages 28321 corresponds to the period during which dermis is formed. The cells that make up the dermis are readily distinguished by their lack of pl-integrin immunostaining. The third phase (7%-10 days of incubation; H.H. stages 33-36) begins with the sudden appearance of pl-integrin in the central and lateral regions of the dermis. The pattern of pl-integrin immunostaining in scaleless backskin becomes different from that of normal backskin during this phase. In normal backskin the dermal condensations of feather germs are not labeled with the pl-integrin antiserum. This produces a heterogeneous immunostaining pattern very similar to the pattern seen for Type I collagen (Mauger et al. [1982] Dev. Biol. 94:93-105). In contrast, homogeneous immunostaining is observed in the dermis of scaleless backskin. The initial time of appearance, manner of appearance, and pattern of integrin expression in the third phase suggest that pl-integrin may be involved in the stabilization of the feather pattern.We also observed the appearance of pl-integrin on the epidermal basal cells during the time of feather follicle formation. The pl-integrin antiserum reacts strongly with the baso-lateral surfaces of normal basal cells, yet the basal surfaces of the scaleless basal cells are unstained. This lack of immunostaining along the basal surfaces of the scaleless basal cells may relate to the abnormal adhesion between the epidermis and dermis in

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Section: Discussionmentioning

confidence: 47%

Section: Introductionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…9E; Mauger et al, 1982). However, the pl-integrin immunostaining pattern is visible at least 1 day before the appearance of the Type I collagen pattern.…”

Section: -71h Days Of Incubation (Hh Stages 28-32)mentioning

confidence: 96%

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Pattern formation in chick feather development: Distribution of β1‐integrin in normal and scaleless embryos

Song

Carver

Sawyer

1994

Developmental Dynamics

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Molecular histology in skin appendage morphogenesis

Widelitz

Jiang

Noveen

et al. 1997

Microsc. Res. Tech.

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Classical histological studies have demonstrated the cellular organization of skin appendages and helped us appreciate the intricate structures and function of skin appendages. At this juncture, questions can be directed to determine how these cellular organizations are achieved. How do cells rearrange themselves to form the complex cyto‐architecture of skin appendages? What are the molecular bases of the morphogenesis and histogenesis of skin appendages? Recently, many new molecules expressed in a spatial and temporal specific manner during the formation of skin appendages were identified by molecular biological approaches. In this review, novel molecular techniques that are useful in skin appendage research are discussed. The distribution of exemplary molecules from different categories including growth factors, intracellular signaling molecules, homeobox genes, adhesion molecules, and extracellular matrix molecules are summarized in a diagram using feather and hair as models. We hope that these results will serve as the ground work for completing the molecular mapping of skin appendages which will refine and re‐define our understanding of the developmental process beyond relying on morphological criteria. We also hope that the listed protocols will help those who are interested in this venture. This new molecular histology of skin appendages is the foundation for forming new hypotheses on how molecules are mechanistically involved in skin appendage development and for designing experiments to test them. This may also lead to the modulation of healing and regeneration processes in future treatment modalities. Microsc. Res. Tech. 38:00–00, 1997. © 1997 Wiley‐Liss, Inc.

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Dorsal dermis of the scaleless (sc/sc) embryo directs normal feather pattern formation until day 8 of development

Song

Sawyer

1996

Dev. Dyn.

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Immunofluorescent localization of collagen types I and III, and of fibronectin during feather morphogenesis in the chick embryo

Cited by 74 publications

References 21 publications

Pattern formation in chick feather development: Distribution of β1‐integrin in normal and scaleless embryos

Pattern formation in chick feather development: Distribution of β1‐integrin in normal and scaleless embryos

Molecular histology in skin appendage morphogenesis

Dorsal dermis of the scaleless (sc/sc) embryo directs normal feather pattern formation until day 8 of development

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