Immunofluorescence study of amyloid P-component patterns in human nephropathies

Orfila, C; Rakotoarivony, J; Segonds, A; Suc, J. M.

doi:10.1007/bf00493434

Cited by 4 publications

(3 citation statements)

References 9 publications

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“…Abnormal patterns of AP distribution have been de scribed in glomerular diseases [11,12]. In our study, glo merulonephritis affected the appearance of AP as com pared to that of normal glomeruli.…”

Section: Discussionsupporting

confidence: 50%

“…Most of our results are consistent with this study, although we ob served a complete absence of AP only in the cases under 6 years of age. However, Orfila et al [ 11 ] did not report the age distribution of their patients.…”

Section: Discussionmentioning

confidence: 91%

“…In our study, glo merulonephritis affected the appearance of AP as com pared to that of normal glomeruli. According to Orfila et al [11], there is great variation in the appearance of AP within one type of glomerulonephritis, and even in cases with only minor glomerular abnormalities. Most of our results are consistent with this study, although we ob served a complete absence of AP only in the cases under 6 years of age.…”

Section: Discussionmentioning

confidence: 99%

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Age-Related Appearance of Amyloid P Component in Human Renal Glomeruli

Rantala

Helin²,

Mustonen³

et al. 1997

Nephron

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Amyloid P component (AP) is a matrix glycoprotein of adult renal glomeruli. To establish whether the deposition of AP in glomeruli is an age-related phenomenon, this study used indirect immunofluorescence (IF) to investigate 34 renal biopsy specimens and 11 renal autopsy specimens. The biopsy specimens were taken from 9 patients (age range from 2 to 38 years) with normal glomerular morphology and from 25 patients (age range from 4 to 56 years) with various renal diseases. All autopsy specimens (age range form 2 months to 28 years) showed normal glomerular morphology. AP was not detected in glomeruli before age 6. By age 14, the IF intensity reached the level of the adult specimens, in which a strong fluorescence was seen along the basement membranes and within the mesangial matrix. In renal diseases, glomerular AP also appeared after age 6, although varying in its location and intensity. In conclusion, our results indicate that the appearance of AP in glomeruli is an age-related phenomenon, the pattern of which varies in renal diseases.

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Section: Discussionsupporting

confidence: 50%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Age-Related Appearance of Amyloid P Component in Human Renal Glomeruli

Rantala

Helin²,

Mustonen³

et al. 1997

Nephron

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Amyloid P-Component: Structure and Properties

Pepys

1986

Amyloidosis

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Familial glomerulonephritis characterized by massive deposits of fibronectin

Assmann

Koene

Wetzels

1995

American Journal of Kidney Diseases

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• In recent years more than 150 cases of glomerulonephritis characterized by deposits of irregularly arranged fibrils have been documented. In the majority of these cases immunoglobulins and complement are the prime constituents of these deposits. We recently made a diagnosis of fibrillary glomerulonephritis without immunoglobu lin deposition in two members of a family, a father and a son. In the father, proteinuria was first discovered 18 years ago. In 1985 he was referred to our outpatient clinic because of hypertension and increasing proteinuria. From that time onward he was regularly seen for blood pressure control, Nephrotlc-range proteinuria persisted, without hardly any evidence of deterioration of renal function. Renal biopsies were performed in 1985 and 1993. His son underwent a renal biopsy in 1993 because of moderate proteinuria. The biopsies of both patients disclosed a distinct form of fibrillary glomerulonephritis that w as characterized by massive deposits of a homogeneous, eosinophilic material in the mesangial and subendothelial areas. Staining for amyloid was negative. Immunofluo rescence revealed that the biopsy specimens only stained faintly for immunoglobulins, complement factors C lq and C3, the extracellular matrix proteins, collagen IV, and laminln, However, they strongly stained for fibronectin. Using monoclonal antibodies specific for cell-derived fibronectin (lST-9) and plasma-and cell-derived fibronectin (IST-4), in the biopsy of the son we demonstrated that the fibronectin deposited in the glomeruli was mainly derived from the plasma, and to a lesser extent from resident glomerular cells. In addition, a moderate staining for amyloid P and vitronectin also was present. No or minor enhanced staining for collagen I, HI, or V, heparan sulfate proteoglycan or its glycosaminoglycan side chains, tenascin, or thrombospondin could be observed. By electron microscopy the deposits in the mesangîum and the subendothelial spaces appeared focally to be com posed of irregularly arranged fibrils or microtubules 10 to 12 nm in diameter. Fibrillary glomerulonephritis with massive deposits of fibronectin represents a rare form of familial glomerulonephritis. In our patients the glomerulo nephritis has an indolent course with hardly any deterioration of renal function.

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Immunofluorescence study of amyloid P-component patterns in human nephropathies

Cited by 4 publications

References 9 publications

Age-Related Appearance of Amyloid P Component in Human Renal Glomeruli

Age-Related Appearance of Amyloid P Component in Human Renal Glomeruli

Amyloid P-Component: Structure and Properties

Familial glomerulonephritis characterized by massive deposits of fibronectin

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