Immunofluorescence localisation of Banana bunchy top virus (family Nanoviridae) within the aphid vector, Pentalonia nigronervosa, suggests a virus tropism distinct from aphid-transmitted luteoviruses

Bressan, Alberto; Watanabe, Shizu

doi:10.1016/j.virusres.2010.12.005

Cited by 32 publications

(36 citation statements)

References 26 publications

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“…As reported in this and previous studies [11,12], the process of BBTV translocation has more similarities with the begomoviruses rather than the luotevirids. In fact, the internalisation of the begomoviruses occurs through the anterior midgut and filter chamber [33,34,35] of B. tabaci .…”

Section: Resultssupporting

confidence: 79%

“…To obtain a more precise assessment of transmission competency, the transmission of BBTV was additionally analyzed using leaf disk assays and individual aphids as previously described [11]. Leaf disk chambers were generated by transversely cutting 15-ml Falcon conical tubes 15 mm below the lid.…”

Section: Methodsmentioning

confidence: 99%

“…The tops of the chambers were sealed with stretched Parafilm ® . At the end of the 2-day IAP, aphids were removed from the chambers, and the leaf disks were kept in the agar, inside a sealed humid chamber to prevent dehydration, for another four days to allow the virus to replicate locally [11]. Transmission assays were carried out in a growth chamber at 25 °C with a photoperiod of 12: 12 (light: dark).…”

Section: Methodsmentioning

confidence: 99%

“…Previously, we have obtained direct evidence of the translocation path of BBTV through the aphid vector. Using immunofluorescence and immunocapture PCR procedures, we showed that BBTV antigens localize to the anterior midgut (AMG) and specific cells of the principal salivary glands (PSGs) [11]. In addition, we reported that BBTV rapidly translocates within the aphid vector; the viral particles first localize to the AMG, where they accumulate and are retained at higher concentrations than in either the haemolymph or the PSGs [12].…”

Section: Introductionmentioning

confidence: 99%

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Localization, Concentration, and Transmission Efficiency of Banana bunchy top virus in Four Asexual Lineages of Pentalonia aphids

Watanabe

Greenwell

Bressan

2013

Viruses

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Banana bunchy top virus (BBTV) is the most destructive pathogenic virus of banana plants worldwide. The virus is transmitted in a circulative non-propagative manner by the banana aphid, Pentalonia nigronervosa Coquerel. In this work, we examined the localization, accumulation, and transmission efficiency of BBTV in four laboratory-established lineages of Pentalonia aphids derived from four different host plants: taro (Colocasia esculenta), heliconia (Heliconia spp.), red ginger (Alpinia purpurata), and banana (Musa sp.). Mitochondrial sequencing identified three and one lineages as Pentalonia caladii van der Goot, a recently proposed species, and P. nigronervosa, respectively. Microsatellite analysis separated the aphid lineages into four distinct genotypes. The transmission of BBTV was tested using leaf disk and whole-plant assays, both of which showed that all four lineages are competent vectors of BBTV, although the P. caladii from heliconia transmitted BBTV to the leaf disks at a significantly lower rate than did P. nigronervosa. The concentration of BBTV in dissected guts, haemolymph, and salivary glands was quantified by real-time PCR. The BBTV titer reached similar concentrations in the guts, haemolymph, and salivary glands of aphids from all four lineages tested. Furthermore, immunofluorescence assays showed that BBTV antigens localized to the anterior midguts and the principal salivary glands, demonstrating a similar pattern of translocations across the four lineages. The results reported in this study showed for the first time that P. caladii is a competent vector of BBTV.

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Section: Resultssupporting

confidence: 79%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Localization, Concentration, and Transmission Efficiency of Banana bunchy top virus in Four Asexual Lineages of Pentalonia aphids

Watanabe

Greenwell

Bressan

2013

Viruses

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“…BBTV is transmitted by banana aphid Pentalonia nigronervosa Coquerel [22,31] in a persistent, circulative and non-propagative manner [8]. The disease is spread long distances by the movement of infected planting material such as suckers, corms and tissue cultured plantlets by humans.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of banana bunchy top virus isolate from Sri Lanka and its genetic relationship with other isolates

et al. 2016

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Bunchy top disease of banana caused by Banana bunchy top virus (BBTV, genus Babuvirus family Nanoviridae) is one of the most important constraints in production of banana in the different parts of the world. Six genomic DNA components of BBTV isolate from Kandy, Sri Lanka (BBTV-K) were amplified by polymerase chain reaction (PCR) with specific primers using total DNA extracted from banana tissues showing typical symptoms of bunchy top disease. The amplicons were of expected size of 1.0-1.1 kb, which were cloned and sequenced. Analysis of sequence data revealed the presence of six DNA components; DNA-R, DNA-U3, DNA-S, DNA-N, DNA-M and DNA-C for Sri Lanka isolate. Comparisons of sequence data of DNA components followed by the phylogenetic analysis, grouped Sri Lanka-(Kandy) isolate in the Pacific Indian Oceans (PIO) group. Sri Lanka-(Kandy) isolate of BBTV is classified a new member of PIO group based on analysis of six components of the virus.

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Plant–Virus–Vector Interactions

et al. 2021

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Immunofluorescence localisation of Banana bunchy top virus (family Nanoviridae) within the aphid vector, Pentalonia nigronervosa, suggests a virus tropism distinct from aphid-transmitted luteoviruses

Cited by 32 publications

References 26 publications

Localization, Concentration, and Transmission Efficiency of Banana bunchy top virus in Four Asexual Lineages of Pentalonia aphids

Localization, Concentration, and Transmission Efficiency of Banana bunchy top virus in Four Asexual Lineages of Pentalonia aphids

Molecular characterization of banana bunchy top virus isolate from Sri Lanka and its genetic relationship with other isolates

Plant–Virus–Vector Interactions

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