Molecular characterization of banana bunchy top virus isolate from Sri Lanka and its genetic relationship with other isolates

Wickramaarachchi, W. A. R. T.; Shankarappa, K. S.; Rangaswamy, K. T.; Maruthi, M. N.; Rajapakse, R. G. A. S.; Ghosh, Saptarshi

doi:10.1007/s13337-016-0311-2

Cited by 13 publications

(8 citation statements)

References 37 publications

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“…5). The use of the replicase gene and the coat protein gene sequences to construct phylogenies has been the prime approach to explain the evolutionary history of BBTV and other Nanoviruses [41]. Phylogenetic analysis showed two broad clades/groups of BBTV, namely, the South East Asian and Paci c Indian Ocean groups, with high bootstrap support values of 96% and 94%, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Banana bunchy top virus (BBTV), a multi-component, circular, single-stranded DNA virus that is 18-20 nm in diameter, is the type member of the genus Babuvirus and the family Nanoviridae [3]. The viral genome comprises at least six encapsidated components (DNA-R; DNA-S; DNA-M; DNA-N; DNA-C and DNA-U3) each approximately 1100 nucleotides in length [41]. Phylogenetic relationships, largely based on the DNA-R, -N and -S segments, grouped BBTV isolates worldwide as 'South Paci c' or 'Asian' origin [20].…”

Section: Introductionmentioning

confidence: 99%

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Monitoring the distribution of banana bunchy top virus in South Africa: a country-wide survey

et al. 2022

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Banana bunchy top disease (BBTD) is the most devastating viral disease of bananas worldwide and is caused by the banana bunchy top virus (BBTV). The disease is spread by the banana aphid Pentalonia nigronervosa Coquerel (Hemiptera: Aphididae) and through infected propagation material. In 2015, the virus was detected in an isolated area in the South Coast region of the KwaZulu-Natal Province (KZN), South Africa. The aim of this study was to conduct surveys across three banana-producing regions in South Africa, viz. KwaZulu-Natal, Mpumalanga and Limpopo provinces. Over 1700 plant and aphid samples were collected from commercial farms and rural households in the three provinces and more intense sampling was done in the affected KZN region. A BBTV-speci c polymerase chain reaction (PCR), targeting the putative replicase gene, was performed to detect virus-infected samples. The amplicons that yielded an expected band size of 349 bp, were sequenced. Comparative phylogenetic analyses showed that the South African BBTV isolates clustered within the Paci c Indian Ocean genomic group that included isolates from India and other regions in Africa with a bootstrap value of 94% . To date, the virus has been identi ed only in the South Coast region of the KwaZulu-Natal province. Intense management strategies, including scouting, removal of infected plants and control of aphids, have been implemented in areas where positive samples were identi ed to minimize the spread of the virus. Findings from this study emphasize the need to continue monitoring and containing the spread in the KZN South Coast region.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Monitoring the distribution of banana bunchy top virus in South Africa: a country-wide survey

et al. 2022

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“…Further, their sequence analysis separated into two genetically distinct taxonomic groups of BBTV are known (Asian and South Pacific) though both have the same vector and produce the same disease symptoms in Musa. The Asian group contains isolates from China, Japan and South-east Asia countries, and the South Pacific group comprises isolates from the rest of the world (including Egypt, India, Sub Saharan Africa and Australia) (Furuya et al 2004(Furuya et al , 2006Selvarajan et al 2010;Stainton et al 2015;Wickramaarachchi et al 2016;Wanitchakorn et al 2000;etc. ).…”

Section: Introductionmentioning

confidence: 99%

Banana Bunchy Top Virus Molecular Confirmation and DNA-S Phylogenetic of some Banana Isolates from Indonesia

2022

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This study was aimed to confirms the Banana Bunchy Top Virus (BBTV) infection of ten banana accessions from Indonesia through PCR assay using primer of BBTV coat protein (CP) gene, also the sequences characteristics and phylogeny. Preliminary morphological results showed five acessions were positively infected with slight to severe intensity symptoms i.e. Pisang Berlin, Candi, Billa, Morosebo, and Mas Kripik; and five accessions were symptomless i.e. M. acuminata var. rutilifes and M. balbisiana, Pisang Ebung, Madu and Moseng. However, PCR results qualitatively confirms that all accessions were positively infected. Two sizes of amplicons were identified. Pisang Candi isolate was showing ±500 bp amplicons, whereas the others were ±1000-1100 bp. The total aligned and selected BBTV CP sequences of 36 accessions (bananas and other host species) was 450 nt. It was considered highly conserved (85.55%), low G+C content (41%), and high genetic similarities (92.47 to 100%). Phylogenetic analysis delineates isolates into two large groups, i.e. the Asian group (East Asia and South-east Asia) and the South Pacific group (South Asia, Africa and the Pacific). The findings of this study are usefull for broader applicability of further banana breeding program particularly for mitigation, selection and evaluation of banana with BBTV resistance.

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“…The disease transmission is of the persistent, circulative, non-propagative type (Anhalt and Almeida, 2008), with efficiency ranging from 46-67% (Magee, 1927; Wu and Su., 1990; Hu et al, 1996). The virus is made up of 6 genetic components, namely, DNA-C coding for the cell cycle link protein, DNA-S coding for the capsid protein, DNA-M coding for the movement protein, DNA-N coding for the nuclear shuttle protein, DNA-U3 coding for potential protein with unknown function, and DNA-R coding for the replication initiation protein (Amin et al, 2008, Kumar et al, 2017; Wickramaarachchi et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Genetic Structure and Diversity of Banana Bunchy Top Virus (BBTV) in the Philippines

Cortaga

et al. 2021

Preprint

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Banana bunchy top virus (BBTV) is an important disease of banana in the Philippines and in other banana-producing countries. This study was conducted to investigate the genetic structure and diversity of Philippine BBTV isolates which remain unexplored in the country. BBTV-infected plant tissues were sampled from banana-growing provinces (i.e., Cagayan, Isabela, Quirino, Batangas, Laguna, Rizal, Quezon, Palawan, Cebu, Leyte, and Davao del Sur) and the partial DNA-R gene of BBTV was sequenced. Analysis of all local BBTV isolates showed a nucleotide diversity (π) of 0.00721, average number of nucleotide differences (k) of 5.51984, and haplotype diversity (hd) of 0.971. Neutrality tests using Fu′s Fs and Tajima′s D showed significant and highly negative values which suggest an excess number of rare alleles due to recent population expansion or from genetic hitchhiking. Haplotype network and phylogenetic analyses revealed that the local BBTV isolates were closely related to Southeast Asian (SEA) group and exhibited a monophyletic clade with distinct haplotype grouping from other SEA sequences. However, some Indonesian and Indian reference sequences were also clustered within the Philippine BBTV group suggesting sequence homology. Results also showed that the local BBTV isolates may be categorized into three major haplotype groups (HA, HB, and HC) but only the HC group remained distinct upon comparison with other Philippine and SEA reference sequences. BBTV isolates from Quezon were the most diverse while isolates from Palawan displayed low genetic diversity indices and belonged only in the HC group. The assessment of the degree of variability among Philippine BBTV isolates will provide a reference towards the development of high-throughput BBTV detection systems as well as enable to devise plant breeding strategies to manage the current BBTV spread and variations.

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Molecular characterization of banana bunchy top virus isolate from Sri Lanka and its genetic relationship with other isolates

Cited by 13 publications

References 37 publications

Monitoring the distribution of banana bunchy top virus in South Africa: a country-wide survey

Monitoring the distribution of banana bunchy top virus in South Africa: a country-wide survey

Banana Bunchy Top Virus Molecular Confirmation and DNA-S Phylogenetic of some Banana Isolates from Indonesia

Genetic Structure and Diversity of Banana Bunchy Top Virus (BBTV) in the Philippines

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