Immunofluorescence in dermatology

Mutasim, Diya F.; Adams, Brian B.

doi:10.1067/mjd.2001.117518

Cited by 142 publications

(166 citation statements)

References 210 publications

(267 reference statements)

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“…With these parameters, a pattern approach can lead to an accurate diagnosis in the majority of specimens (25). In the case, that DIF is done during the treatment by immunosupresives, the results of DIF can be negative.…”

Section: Discussionmentioning

confidence: 99%

A study of clinical, histopathological and direct immunofluorescence diagnosis in pemphigus group Utility of direct immunofluorescence

Hrabovska¹,

Jautová²,

Hrabovsky³

2017

BLL

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AIMS: To determine the diagnostic accordance between histopathological and direct immunofl uorescence diagnosis of patients with autoimmune vesiculobullous skin diseases. BACKGROUND: The term pemphigus refers to a group of autoimmune blistering diseases mediated by autoantibodies directed against desmoglein proteins. The differentiation between the various bullous diseases is important for treatment and prognosis. Direct immunofl uorescence microscopy is still the gold standard in differentiating these diseases. METHODS: Patients with clinical diagnosis of vesiculobullous dermatitis from pemphigus group were included in the study. We retrospectively analyzed histopathologic and direct immunofl uorescence results from skin or mucosal samples over 15-year period. RESULTS: 81 patients were included. The accordance was good in pemphigus vulgaris and pemphigus herpetiformis, but low in pemphigus vegetans, pemphigus foliaceus and pemphigus erythematosus. No accordance was in Hailey-Hailey disease and Grover´s disease. Uncommon result in our analysis included: intraepidermal IgG and IgM depositions at DIF in one Grover´s disease patient. CONCLUSION: Our results confi rmed the relevance of direct immunofl uorescence assays as a necessary diagnostic method for the defi nitive diagnosis of autoimmune blistering disease in the case, where the clinical feature and the results of histopathology are not clear (Tab. 4, Fig. 5, Ref. 26). Text in PDF www.elis.sk.

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Section: Discussionmentioning

confidence: 99%

A study of clinical, histopathological and direct immunofluorescence diagnosis in pemphigus group Utility of direct immunofluorescence

Hrabovska¹,

Jautová²,

Hrabovsky³

2017

BLL

View full text Add to dashboard Cite

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“…İİF'de farklı büllöz hastalıkların tanısında substrat olarak kullanılan antijenler insan derisi, maymun özofagusu, gine domuzu dudak ve özofagusu, sıçan mesanesi ve tuzda ayrıştırılmış insan derisidir (TAİD) (7). Substratların sensivitesi ve spesifitesi farklı büllöz hastalıklarda değişkenlik gösterir (7,8).…”

Section: İndirekt İmmünofloresanunclassified

“…Substratların sensivitesi ve spesifitesi farklı büllöz hastalıklarda değişkenlik gösterir (7,8). Desmoglein 3'den (Dsg) zengin maymun özofagusu pemfigus vulgarisin (PV) IgG otoantikorlarını göstermede en sensitif substrattır (8).…”

Section: İndirekt İmmünofloresanunclassified

“…Pemfigoid gestasyonesde İİF pozitifliği %10-30 kadardır (7,19). İİF ile %25 olguda dolaşan anti-BMZ IgG antikorları saptanırken %50-75 olguda da komplemanı fikse eden anti-BMZ IgG antikorları (HG faktör) saptanır (7,11).…”

Section: Pemfigoidunclassified

“…İİF ile %25 olguda dolaşan anti-BMZ IgG antikorları saptanırken %50-75 olguda da komplemanı fikse eden anti-BMZ IgG antikorları (HG faktör) saptanır (7,11). HG faktör testi rutin İİF yöntemlerden daha zor uygulanabilir bir test ve DİF pozitif hastalarda düşük tanısal değere sahip olduğundan, pemfigoid gestasyones düşünülen ve tanısı histopatoloji ve DİF ile doğrulanamamış hastalarda uygulanabilir.…”

Section: Pemfigoidunclassified

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