Immunoexpression of Tumor Infiltrating Lymphocytes(TILS) CD4 + and CD8 + in Oral Squamous Cell Carcinoma (OSCC) in Correlations with Clinicopathological Characteristics and Prognosis

Abstract

Objective: This study aimed to analyze CD4 +and CD8 + TILs in oral squamous cell carcinoma (OSCC) and to correlate it with histologic grade of malignancy and clinicopathologic data. Methods: The sample was composed of 43 archived specimens. Clinical features and histological grade of malignancy were obtained. The infiltrating intensity of CD4 +, CD8 positive cells were assessed by immunohistochemistry. One-way ANOVA was used to study the association between CD4 +, CD8 + and the grade of OSCC. The cut-off values of the proposed diagnostic indices were received from calculating the coordinates of the receiver operating characteristic (ROC) curve. For clinicopathologic data Independent-Samples T test, Pearson Correlation Coefficient, Correlation Coefficient were used clinicopathologic characteristics. Results: CD4 +and CD8 + were observed in all specimens. CD4 + were more frequent in poorly differentiated specimens (74.14) (P= 0.021<0.05). CD8 + were more frequent in well-differentiated specimens (51.18). None of these correlations were significant (P=0.454>0.05). CD4 +/ CD8 ratio was higher in low-grade specimens (180.28) (P=0.017<0.05). No differences between CD4 +, CD8 +and CD4 +/ CD8 ratio between poorly-differentiated and moderately-differentiated groups ROC P value (0.370, 0.248, 0.126) respectively. there is a difference between CD4 +, CD4 +/ CD8ratio between poorly-differentiated and well-differentiated groups ROC P value (0.022, 0.341, 0.012) Sensitivity (0.857, 0.882), specificity (0.706, 0.857) respectively. and no differences between CD8 + poorlydifferentiated and well-differentiated groups ROC P value (0.341). there is a difference between CD4 + between moderately -differentiated and well-differentiated groups ROC P value (0.038) Sensitivity (0.368), specificity (0,765). No significant correlation was obtained with clinicopathologic findings of OSCC. Conclusion: CD4 + and CD4 +/ CD8 + ratio are independent prognostic factor in OSCC.

Expression of CD4+ and CD8+ Tumor-Infiltrating Lymphocytes in Oral Squamous Cell Carcinoma and Their Relationship With Clinicopathological Parameters: A Cross-Sectional Study

“…In this study, CD4+ and CD8+ expression was not significantly associated with clinical parameters such as age, gender, tobacco habit, or the tumor site. The results of the study were similar to those of a study by Wahbi and Manadili [ 8 ], where it was reported that CD4+ and CD8+ expression was not significantly associated with age, gender, and tobacco smoking. However, this is contrary to a study by Lequerica-Fernández et al [ 5 ], in which there were higher CD4+ and CD8+ TILs in patients without tobacco smoking and alcohol consumption.…”

“…Fang et al [22] investigated the prognostic significance of TILs in OSCC and concluded that higher CD8+ expression was associated with the absence of regional lymph node metastasis. In contrast, in the study by Wahbi and Manadili [8], CD4+ and CD8+ infiltration did not show any significant association with the TNM stage. Similarly, Mohajertehran et al [23] reported no significant association between CD4+ infiltration and the TNM stage.…”

“…The immune microenvironment primarily comprises TILs such as T lymphocytes, B lymphocytes, and natural killer cells [ 7 ]. Anti-tumor immunity is represented by varied subsets of TILs such as CD4+ and CD8+ T cells [ 2 , 3 , 8 ]. CD4+ T lymphocytes, also known as T helper cells, are versatile multifunctional cells that coordinate adaptive T-cell immunity.…”

Evaluating CD4 and Foxp3 mRNA Expression in Tissue Specimens of Celiac Disease and Colorectal Cancer Patients

“…As CD4 + T cells are also crucial in the development of CD, their uncontrolled activation within the CD context may lead to the emergence of CRC [ 31 ]. Wahbi et al [ 32 ] analyzed the presence of CD4 + and CD8+ Tumor Infiltrating Lymphocytes (TILs) in oral squamous cell carcinoma (OSCC) and their correlation with malignancy grade. CD4 + TILs were more frequent in poorly differentiated specimens, while CD8+ TILs were more frequent in well-differentiated specimens.…”

“…CD4 + TILs were more frequent in poorly differentiated specimens, while CD8+ TILs were more frequent in well-differentiated specimens. The CD4 +/CD8+ ratio was higher in low-grade specimens, suggesting that CD4 + and CD4 +/CD8+ ratio are independent prognostic factors in OSCC [ 32 ]. Salama et al [ 33 ] investigated the correlation between PDL1 expression and clinicopathological parameters in endometrial cancer (EC) cases, focusing on CD4 and CD8 immune cells.…”