Immunoenzymatic test for Q-fever

Rogés, German; Edlinger, E

doi:10.1016/0732-8893(86)90146-x

Cited by 9 publications

(7 citation statements)

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“…Although comparisons of the IFA test with the complement fixation and microagglutination tests have been made, the specificity and sensitivity of the IFA test to detect past infections with C. bumetii are still undetermined. The IFA test is not considered the optimal test for epidemiologic surveys of Q fever because the antibodies decay rapidly and the test is subjective and is not easily adapted for testing large numbers of specimens (30). The indirect ELISA is another test widely used for serodiagnosis of Q fever, especially in animals (3).…”

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confidence: 99%

“…The indirect ELISA is another test widely used for serodiagnosis of Q fever, especially in animals (3). The ELISA is highly sensitive, easy to perform, has great potential adaptability for automation, can be applied in epidemiologic surveys (3,11,30), and has been shown to be of value for the diagnosis of acute and chronic Q fever (10). The specificity of the ELISA for epidemiologic surveys of human Q fever is important to ensure that appropriate treatment, preventive, and control measures can be recommended.…”

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Evaluation of specificity of indirect enzyme-linked immunosorbent assay for diagnosis of human Q fever

et al. 1994

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Ninety-five acute-and convalescent-phase serum specimens from 48 patients suspected of having rickettsial or Legionella infections were assayed for antibodies to Coxiella burnetii, the causative agent of Q fever. To evaluate the specificity of the indirect enzyme-linked immunosorbent assay (ELISA) for human Q fever, we compared the ELISA results with those of the indirect immunofluorescence antibody (IFA) test. The ELISA data were analyzed by two different criteria for a positive test. The first criterion for positive results by ELISA was based upon diagnostic titers established in a study of 150 subjects who had no demonstrable cellular or humoral immune responses to C. burnetii phase I or phase II whole cells or phase I lipopolysaccharide. The second criterion was based upon diagnostic antibody titers in a study of 51 subjects who had been diagnosed as having clinical Q fever and had fourfold or greater rises in humoral immune responses to C. burnetii phase I and phase II whole-cell antigens. A comparison of the ELISA and IFA test results of the 95 serum specimens indicated excellent agreement between the tests (Kappa = 92.9%; P < 0.05). None of the 38 patients whose etiologies were confirmed serologically as Legionnaires' disease or rickettsial diseases other than Q fever were classified as positive for C. burnetii by the ELISA. Only one patient identified by the IFA test as having Q fever was not scored positive by the ELISA. These results suggest that the ELISA is useful for epidemiologic screening and as a diagnostic test for human Q fever.

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Evaluation of specificity of indirect enzyme-linked immunosorbent assay for diagnosis of human Q fever

et al. 1994

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“…Since 1983, a few papers have dealt with immunoenzymatic tests for the diagnosis of Q fever (8,11,21). In this paper, we describe the enzyme-linked immunosorbent assay (ELISA) and compare it with the CF and IFA tests to detect human immunoglobulin G (IgG) to C. burnetii phase II.…”

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Comparison of enzyme-linked immunosorbent assay and complement fixation and indirect fluorescent-antibody tests for detection of Coxiella burnetii antibody

Péter¹,

Dupuis²,

Peacock³

et al. 1987

J Clin Microbiol

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An enzyme-linked immunosorbent assay (ELISA) was developed to detect immunoglobulin G to Coxiella burnetii phase II. Serum samples from 213 patients who had had Q fever 1 year previously and from 301 blood donors from six localities in Switzerland were tested by ELISA and by indirect fluorescent-antibody (IFA) and complement fixation (CF) tests. The ELISA and the IFA and CF tests detected antibody to C. burnetii in 202 (94.8%), 193 (90.6%), and 166 (77.8%) of the 213 Q fever patients, respectively. With the serum samples from blood donors, the ELISA yielded a higher percentage of positive sera than did the IFA and CF tests. The high specificity of the three tests was confirmed by analyzing paired serum samples from 36 patients suffering from acute pneumonia of viral or bacterial origin. In these cases, the serological results were negative by the three tests, except for three Q-fever cases included as positive control.

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“…The diagnosis of Q fever is mainly based on indirect serological procedures such as complement fixation test (CFT), enzyme-linked immunosorbent assay (ELISA) (3,15,17,21,23) or immunofluorescence (IF) (6,13). Immunoblotting as a diagnostic tool has been introduced to C. burnetii serology only recently (4,9,10,20).…”

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Monoclonal antibody based capture ELISA/ELIFA for detection of Coxiella burnetii in clinical specimens

1992

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A CAPTURE ELISA/ELIFA system based on monoclonal capture and biotinylated monoclonal detection antibody is described. The assay is fast, highly specific and detects a minimum dose of 2500 Coxiella (C.) burnetii particles. In contrast to the sophisticated and cumbersome isolation procedures, even non-specialized laboratories could use this assay system for investigating clinical samples of different origin for C. burnetii within a short period of time.

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Immunoenzymatic test for Q-fever

Cited by 9 publications

References 8 publications

Evaluation of specificity of indirect enzyme-linked immunosorbent assay for diagnosis of human Q fever

Evaluation of specificity of indirect enzyme-linked immunosorbent assay for diagnosis of human Q fever

Comparison of enzyme-linked immunosorbent assay and complement fixation and indirect fluorescent-antibody tests for detection of Coxiella burnetii antibody

Monoclonal antibody based capture ELISA/ELIFA for detection of Coxiella burnetii in clinical specimens

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