Evaluation of specificity of indirect enzyme-linked immunosorbent assay for diagnosis of human Q fever

Uhaa, I. J.; Fishbein, Daniel B.; Olson, James G.; Rives, Cornelia C.; Waag, David M.; Williams, Jim C.

doi:10.1128/jcm.32.6.1560-1565.1994

Cited by 36 publications

(13 citation statements)

References 36 publications

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“…Moreover, serology allows the differentiation of acute and chronic Q fever infections. Methods which have been used include microagglutination (103,163,252), complement fixation (147,246,270), radioimmunoassay (80), IFA (100,270), indirect hemolysis test (370), ELISA (174,272,378,387,406), enzyme-linked immunosorbent fluorescence assay (ELIFA) (320), dot immunoblotting, and Western blotting (35,399). The techniques most commonly used include complement fixation, IFA, ELISA, and microagglutination.…”

Section: Serologymentioning

confidence: 99%

Q Fever

1999

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SUMMARY Q fever is a zoonosis with a worldwide distribution with the exception of New Zealand. The disease is caused by Coxiella burnetii, a strictly intracellular, gram-negative bacterium. Many species of mammals, birds, and ticks are reservoirs of C. burnetii in nature. C. burnetii infection is most often latent in animals, with persistent shedding of bacteria into the environment. However, in females intermittent high-level shedding occurs at the time of parturition, with millions of bacteria being released per gram of placenta. Humans are usually infected by contaminated aerosols from domestic animals, particularly after contact with parturient females and their birth products. Although often asymptomatic, Q fever may manifest in humans as an acute disease (mainly as a self-limited febrile illness, pneumonia, or hepatitis) or as a chronic disease (mainly endocarditis), especially in patients with previous valvulopathy and to a lesser extent in immunocompromised hosts and in pregnant women. Specific diagnosis of Q fever remains based upon serology. Immunoglobulin M (IgM) and IgG antiphase II antibodies are detected 2 to 3 weeks after infection with C. burnetii, whereas the presence of IgG antiphase I C. burnetii antibodies at titers of ≥1:800 by microimmunofluorescence is indicative of chronic Q fever. The tetracyclines are still considered the mainstay of antibiotic therapy of acute Q fever, whereas antibiotic combinations administered over prolonged periods are necessary to prevent relapses in Q fever endocarditis patients. Although the protective role of Q fever vaccination with whole-cell extracts has been established, the population which should be primarily vaccinated remains to be clearly identified. Vaccination should probably be considered in the population at high risk for Q fever endocarditis.

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Section: Serologymentioning

confidence: 99%

Q Fever

1999

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“…Since the clinical diagnosis is difficult, in most instances, the diagnosis of Q fever relies upon serology. Several methods have been described: microagglutination (46,81,123), complement fixation (66,120,134), radioimmunoassay (33), indirect immunofluorescence antibody tests (immunofluorescence assay) (44,134), indirect haemolysis test (183), ELISA (84,133,188,191,201), enzymelinked immunosorbent fluorescence assay (163), dot immunoblotting, and Western immunoblotting (10,198). Criteria to be taken into account in choosing a diagnostic test include its specificity, sensitivity, positive predictive value, cost, and the amount of antigen required.…”

Section: Specific Laboratory Diagnosismentioning

confidence: 99%

Diagnosis of Q Fever

1998

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“…Routine diagnosis of Q fever is mainly established by serological tests including indirect immunofluorescence (IF) test (2,9), complement fixation (CF) test (11,12) and enzyme-linked immunosorbent assay (ELISA) (23,25). which can be used for detecting the antibodies against C. burnetii antigens.…”

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confidence: 99%

Evaluation of a Recombinant 27‐kDa Outer Membrane Protein of Coxiella burnetii as an Immunodiagnostic Reagent

Zhang

Hotta

et al. 1998

Microbiology and Immunology

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The 27-kDa outer membrane protein from eight strains of Coxiella burnetii was expressed in the pET-21c protein expression system. Two fusion proteins with molecular masses of 30 and 32 kDa were evident in all eight of the recombinants by SDS-PAGE and immunoblotting. A protein having an approximate size of 30 kDa was purified from the Escherichia coli lysates by one-step affinity purification. The utility of the purified recombinant protein in ELISA was also evaluated by testing its reactivity with human sera and comparing this reactivity with that of Nine Mile phase II antigen. All of the 40 IF-positive serum samples were ELISA-positive for both the Nine Mile phase II and recombinant antigens, and negative serum controls were negative for both antigens. These results suggest that ELISA with the 27-kDa recombinant antigen is a sensitive and specific method for detecting anti-C. burnetii antibodies in human sera.

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Evaluation of specificity of indirect enzyme-linked immunosorbent assay for diagnosis of human Q fever

Cited by 36 publications

References 36 publications

Q Fever

Q Fever

Diagnosis of Q Fever

Evaluation of a Recombinant 27‐kDa Outer Membrane Protein of Coxiella burnetii as an Immunodiagnostic Reagent

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