Immunoenhancement effect of rehmannia glutinosa polysaccharide on lymphocyte proliferation and dendritic cell

Huang, Yee; Jiang, Chunmao; Hu, Yuanliang; Zhao, Xiaojuan; Cheng, Saifeng; Yu, Yun; Liu, Cui; Tao, Yang; Pan, Huarong; Feng, Yongzeng; Liu, Jiaguo; Wu, Yi; Wang, Deyun

doi:10.1016/j.carbpol.2013.04.018

Cited by 106 publications

(48 citation statements)

References 40 publications

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“…Splenic lymphocyte proliferation is a crucial event in the activation cascade of both cellular and humoral immune responses. Many reports suggested that antitumor activities of natural medicines were mediated through enhancing the immune responses (Huang et al, 2013). Therefore, in this study, the effects of the isolated compounds from SCP on splenic lymphocyte proliferation were determined.…”

Section: Splenic Lymphocyte Proliferative Activity Of the Isolated Comentioning

confidence: 97%

“…1 H, 13 C NMR and 2D NMR spectra were recorded on a JEOLJNM-ECP600 MHz NMR spectrometer with TMS as an internal standard. ESI-MS spectra were obtained from a Micromass Q-TOF spectrometer.…”

Section: General Experimental Proceduresmentioning

confidence: 99%

“…Their structures were elucidated using comprehensive spectroscopic methods including 1D and 2D NMR, MS, UV and IR, combined with a variety of chemical methods, and by comparison their data with those reported previously in the literature. The isolated compounds were involved in ten types of chemical structures, including fourteen flavonoid glycosides (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14), ten flavonoids (15)(16)(17)(18)(19)(20)(21)(22)(23)(24), four lignans (25)(26)(27)(28), seven phenolic acid compounds (29-34, 42), one steroid (35), one diterpene (36), one monoterpene (37), one phenolic glycoside (38), three iridoid glycosides (39)(40)(41) and one steroid saponin (43) ( Table 1). Among the isolated compounds, about of 50% of them were flavonoid glycosides and flavonoids, and it could be concluded that flavonoid glycosides, flavonoids, phenolic compounds and lignans were the major structure types in the EtOAc and n-BuOH fractions of SCP.…”

Section: Chemical Constituents Of the Etoac And N-buoh Fractions Frommentioning

confidence: 99%

“…As a result, flavonoids and caffeic acid derivatives in SCP possessed excellent DPPH radical scavenging activities. (Huang et al, 2013). Therefore, preliminary assessment for activation extent of T cells could be made by detecting the contents of IFN-γ and IL-2.…”

Section: Dpph Radical Scavenging Activity Of the Isolated Compoundsmentioning

confidence: 99%

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Screening and identification of potential bioactive constituents in a new herbal prescription seaweed complex preparation

Liu¹,

Wen²,

Sun³

et al. 2016

Afr. J. Trad. Compl. Alt. Med.

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Background: Seaweed Complex Preparation (SCP) is a clinical traditional Chinese medicine preparation composed of seven traditional Chinese herbs, and has been used for treatment of lung cancer, liver cancer and digestive cancer. Materials and Methods: In this study, the potential bioactive constituents of SCP were isolated and identified by chromatographic and spectroscopic methods. The immunomodulatory and DPPH radical scavenging activities of the constituents were also evaluated in vitro. Results: Forty-three compounds were isolated from the EtOAc and n-BuOH fractions of the decocted extracts. More than 10 structural types of chemical constituents were found, including flavonoid glycosides, flavonoids, phenolic acid compounds, lignans, iridoid glycosides, and polysaccharides mainly composed of galactose and glucose. Flavonoid glycosides exhibited potential immunomodulatory by promoting splenic lymphocyte proliferation and stimulating the secretion of cytokines IL-2 and IFN-γ, as well as free radical (DPPH) scavenging activities. Caffeic acid derivatives showed remarkable free radical (DPPH) scavenging activity, while polysaccharides also displayed immunological enhancement effects. Conclusion: Four molecular communities, flavonoid glycosides, flavonoids, phenolic compounds and polysaccharides, might be the potential bioactive components of SCP. Synergism of these four molecular communities in SCP may endue the anticancer properties and reflect the feature of multi-components and multi-targets of traditional Chinese medicine formula.

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Section: Splenic Lymphocyte Proliferative Activity Of the Isolated Comentioning

confidence: 97%

“…1 H, 13 C NMR and 2D NMR spectra were recorded on a JEOLJNM-ECP600 MHz NMR spectrometer with TMS as an internal standard. ESI-MS spectra were obtained from a Micromass Q-TOF spectrometer.…”

Section: General Experimental Proceduresmentioning

confidence: 99%

Section: Chemical Constituents Of the Etoac And N-buoh Fractions Frommentioning

confidence: 99%

Section: Dpph Radical Scavenging Activity Of the Isolated Compoundsmentioning

confidence: 99%

See 2 more Smart Citations

Screening and identification of potential bioactive constituents in a new herbal prescription seaweed complex preparation

Liu¹,

Wen²,

Sun³

et al. 2016

Afr. J. Trad. Compl. Alt. Med.

View full text Add to dashboard Cite

show abstract

“…Primary bone marrow DCs were obtained from mouse bone marrow precursors as previously described, 8,19 with minor modifications. DCs were cultured at a starting concentration of 2.0×10 6 cells/mL in round-bottomed 6-well plates with RPMI 1640 supplemented with 10% FCS (Gibco, Waltham, MA, USA).…”

Section: Analysis Of the Effect Of Rgpl On Dcs Generation Of Bone Marmentioning

confidence: 99%

<em>Rehmannia glutinosa</em> polysaccharide liposome as a novel strategy for stimulating an efficient immune response and their effects on dendritic cells

Huang¹,

Qin²,

Huang³

et al. 2016

IJN

Self Cite

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Rehmannia glutinosa exhibits anti‐aging effect through maintaining the quiescence and decreasing the senescence of hematopoietic stem cells

Bai

Shi

Yang

et al. 2018

Anim Models and Exp Med

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Background The time‐related decline in regenerative capacity and organ homeostasis is a major feature of aging. Rehmannia glutinosa and Astragalus membranaceus have been used as traditional Chinese herbal medicines for enhanced immunity and prolonged life. However, the mechanism by which this herbal medicine slows aging is unknown. In this study, we investigated the mechanism of the herbal anti‐aging effect. Methods Mice were fed diets supplemented with R. glutinosa or A. membranaceus for 10 months; the control group was fed a standard diet. The phenotypes were evaluated using a grading score system and survival analysis. The percentages of the senescence phenotypes of hematopoietic stem cells ( HSC s) were determined by fluorescence‐activated cell sorting analysis. The function and the mechanism of HSC s were analyzed by clonogenic assay and the real‐time polymerase chain reaction. Results The anti‐aging effect of R. glutinosa is due to the enhanced function of HSC s. Mice fed with R. glutinosa displayed characteristics of a slowed aging process, including decreased senescence and increased rate of survival. Flow cytometry analysis showed decreased numbers of Lin – Sca1 + c‐kit – ( LSK ) cells, long‐term HSC s ( LT ‐ HSC s) and short‐term HSC s ( ST ‐ HSC s) in the R. glutinosa group. In vitro, clonogenic assays showed increased self‐renewal ability of LT ‐ HSC s from the R. glutinosa group as well as maintaining LSK quiescence through upregulated p18 expression. The R. glutinosa group also showed decreased reactive oxygen species levels and the percentage of β‐gal + cells through downregulation of the cellular senescence‐associated protein p53 and p16. Conclusion Rehmannia glutinosa exerts anti‐aging effects by maintaining the quiescence and decreasing the senescence of HSC s.

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Immunoenhancement effect of rehmannia glutinosa polysaccharide on lymphocyte proliferation and dendritic cell

Cited by 106 publications

References 40 publications

Screening and identification of potential bioactive constituents in a new herbal prescription seaweed complex preparation

Screening and identification of potential bioactive constituents in a new herbal prescription seaweed complex preparation

<em>Rehmannia glutinosa</em> polysaccharide liposome as a novel strategy for stimulating an efficient immune response and their effects on dendritic cells

Rehmannia glutinosa exhibits anti‐aging effect through maintaining the quiescence and decreasing the senescence of hematopoietic stem cells

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