Immunoelectronmicroscopy for Virus Detection and Virus Strain Identification

Kerlan, Camille; Mille, B.; Dunez, Jean

doi:10.17660/actahortic.1983.130.29

Cited by 4 publications

(2 citation statements)

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“…Several strains of PPV have been discovered, all genetically distinctive with serological particularities and biological properties including natural host range, relative infectivity and symptomatology in natural and artificial host species. The first two PPV strains have been described as serotypes PPV-D and PPV-M showing different reactivity with antibodies in immunodiffusion tests (Kerlan and Dunez, 1979). As these serotypes were found to be vastly prevalent in Europe, PPV variability has been considered to be very low for a long time (Revers et al, 1996).…”

Section: Ppv Strain Variabilitymentioning

confidence: 99%

Unfolding the secrets of plum pox virus: from epidemiology to genomics

Šubr¹,

Glasa²

2013

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Summary. -Over an approximately 80-year period since the description of the Sharka disease, the plum pox virus (PPV) has been thoroughly studied on various levels of the infection cycle. World-wide distribution of the virus, severity of the disease for the fruit industry with a potential to be further increased and discovery/ emergence of new strains make PPV the most epidemiologically important viral pathogen of stone fruit trees. The history of PPV research reflects the development of analytical methods applicable in plant virology. In particular the establishment of molecular biology with reverse genetics and improvement of DNA sequencing technology have further contributed to the increase in knowledge on PPV variability, evolution, replication and interaction with host plants. This review gives a comprehensive summary of PPV data accumulated progressively, from the biological characterization of the disease to recent attempts aimed at using the PPV-based vectors for expression of exogenous proteins in plants.

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Section: Ppv Strain Variabilitymentioning

confidence: 99%

Unfolding the secrets of plum pox virus: from epidemiology to genomics

Šubr¹,

Glasa²

2013

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“…GF 305) were inoculated by double chip budding with a PPV sero-group M collected in Greece [17]. Two green bark chip buds were inserted into both the rootstocks and the transgenic scions.…”

Section: Ppv Inoculationmentioning

confidence: 99%

Role of the 25–26 nt siRNA in the resistance of transgenic Prunus domestica graft inoculated with plum pox virus

et al. 2007

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The reaction of a genetically engineered plum clone (C5) resistant to plum pox virus (PPV) by graft inoculation with the virus was evaluated. The resistance in this clone has been demonstrated to be mediated through post-transcriptional gene silencing (PTGS). A single C5 plant out of 30 plants inoculated with PPV M strain by double chip-budding showed mild diffuse mosaic 'Sharka' symptom at the bottom section of the scion. The upper leaves of this PPV-infected C5 plant remained symptomless and the virus was not detected in them by either DAS-ELISA or RT-PCR. An RNA silencing associated small interfering RNA duplex, siRNA (21-26 nt), was detected in non-inoculated C5 plants and in the portions of inoculated C5 plant in which PPV could not be detected. In the PPV-infected portion of the C5 plant and in C6 PPV susceptible plants only the approximately 21-22 nt siRNAs was detected. Cytosine-methylation was confirmed in C5 plants both uninfected and showing PPV symptoms. The 25-26 nt siRNA normally present in C5 was absent in PPV-infected C5 tissues confirming the critical role of this siRNA in the resistance of clone C5 to PPV infection. We also show that this PPV infection was limited and transient. It was only detected in one plant at one of four post-dormancy sampling dates and did not appear to affect the overall PPV resistance of the C5 clone.

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Immunoelectron Microscopy of Plant Viruses and Mycoplasmas

Milne

1992

Advances in Disease Vector Research

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Immunoelectronmicroscopy for Virus Detection and Virus Strain Identification

Cited by 4 publications

References 0 publications

Unfolding the secrets of plum pox virus: from epidemiology to genomics

Unfolding the secrets of plum pox virus: from epidemiology to genomics

Role of the 25–26 nt siRNA in the resistance of transgenic Prunus domestica graft inoculated with plum pox virus

Immunoelectron Microscopy of Plant Viruses and Mycoplasmas

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