Immunodiffusion and Immuno-histochemical Investigations on the Reactivity of Oxide Ceramic Middle-Ear Implants

Abstract

Oxide ceramic materials (partial and total ossicular replacement prostheses) have been implants of preference for the reconstruction of the ossicular chain because of their excellent biocompatibility. The reaction on the surface of the implants takes place at three biodynamic levels according to the model of Stern’s bilayer. We investigated the adsorption of proteins, which is determined by the cellular reaction and degradation to the surface using radial immunodiffusion and immunohistochemical methods. First, ceramic implants of aluminum oxide, hydroxyapatite, glass ceramic and zirconium oxide have individual actual (i.e. biological) surfaces. With a perthometer and the contact-free laser Focodyn method we determined each actual (i.e. biological) surface of the various ceramic implants mentioned above. Using radial immunodiffusion, the adsorption of albumin, glycoprotein, plasminogen, fibronectin, IgA, IgG and IgM shows characteristic rates of adsorption to the respective ceramic surfaces in correlating to the actual surface. A cross-check with fluorescent antibodies confirmed the protein adsorption. The individual surface adsorption of the proteins remains characteristic and is the basis for the recording of cellular reactions after implantation.

The elements of life and medicines

“…Use of zirconium for biomedical applications is steadily growing [ 146 ], e.g. in dental implants [ 147 , 148 ], total knee and hip replacements [ 149 ] and middle-ear ossicular chain reconstruction surgery [ 150 ]. Like Al III , Zr IV readily forms polymeric oxygen-bridged complexes (e.g.…”

Protein adsorption onto ceramic surfaces

“…Although the combination of ''ceramic-polyethylene'' has proven to be clinically reliable in artificial joints over a period of ten years, 13 there is little experience in using these materials in a blood-contacting device. Although a few studies have been published with regard to ce-ramic-protein interactions, 14,15 this study was undertaken to investigate protein adsorption, especially albumin, IgG, fibrinogen, and fibronectin, onto ceramic surfaces.…”

Estudo experimental da resposta tecidual à presença de celulose produzida por Acetobacter xylinum no dorso nasal de coelhos

“…Na literatura observamos que, para a avaliação da biocompatibilidade do enxerto em relação ao tecido hospedeiro, existem vários métodos. Têm-se: cultura de células e tecidos, análise histoquímica 12 , estudos bioquímicos 13,14 , estudos histológicos e estudos de perfusão de todo um órgão. Existem também medidas de peso, rigidez, elasticidade, alongamento, quebras na mecânica e alterações na superfície que podem ser revelados na microscopia eletrônica.…”