Immunodiagnosis of Human Leptospirosis by Dot-Elisa for the Detection of IgM, IgG, and IgA Antibodies

Mv, da Silva; Pm, Nakamura; Ed, Camargo; Batista, Luiza Ivete Vieira; Aj, Vaz; Ec, Romero; Ap, Brandão

doi:10.4269/ajtmh.1997.56.650

Cited by 29 publications

(30 citation statements)

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“…Se sabe que los anticuerpos IgG anti-Leptospira detectados por la técnica de MAT permanecen detectables por aproximadamente 6 a 12 meses después de la exposición a la infección 23,24 , e incluso pueden durar por años. La presencia de personas mayores de 15 años con niveles de anticuerpos IgG en la población estudiada sugiere que la circulación de Leptospira en la región de Urabá es activa y constante.…”

Section: Discussionunclassified

Situación de la leptospirosis en el Urabá antioqueño colombiano: estudio seroepidemiológico y factores de riesgo en población general urbana

Agudelo‐Flórez¹,

Restrepo-Jaramillo²,

Arboleda³

2007

Cad. Saúde Pública

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Leptospirosis es una zoonosis de gran incidencia en regiones tropicales. Su prevalencia es desconocida en la región del Urabá colombiano. Entre marzo y octubre del año 2000 se realizó un estudio descriptivo de corte para determinar la seroprevalencia de anticuerpos contra Leptospira spp. y describir algunos factores de riesgo en nueve municipios del Urabá. La población incluida fue de 582 personas a las cuales se les tomó una muestra de sangre y se le aplicó una encuesta sobre factores de riesgo. La detección de anticuerpos contra Leptospira spp. fue realizada por inmunofluorescencia indirecta y por microaglutinación. La seroprevalencia general en la zona fue 12,5% (IC95%: 10,01-15,5). No hubo diferencias en cuanto al sexo, raza, oficio, edad, años de residencia en la zona y características de la vivienda. L. interrogans serovar Grippotyphosa fue la especie más prevalente, identificándose en 53 de los seropositivos. En 38 seropositivos los títulos detectados fueron iguales o mayores a 1:400. En conclusión, existe alta prevalencia de anticuerpos contra Leptospira spp. Es necesario orientar las medidas de control para disminuir el riesgo de exposición ambiental a leptospirosis por parte de los habitantes de la zona.

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Section: Discussionunclassified

Situación de la leptospirosis en el Urabá antioqueño colombiano: estudio seroepidemiológico y factores de riesgo en población general urbana

Agudelo‐Flórez¹,

Restrepo-Jaramillo²,

Arboleda³

2007

Cad. Saúde Pública

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“…Several other alternatives of antibody detection assays have subsequently been developed for early diagnosis of leptospirosis. These include the hemolytic test (8), the slide agglutination test (14), the indirect hemagglutination assay (19,28), the indirect immunofluorescence test (32), the microcapsule agglutination test (2), the indirect enzyme-linked immunosorbent assay (ELISA) for immunoglobulin M (IgM) antibodies (1,22), the dot-ELISA for IgM (23,26,36), the LEPTO Stick (15), and the lateral flow assay (27). While these methods are much simpler than the MAT and many of them are currently available commercially, they still need a lag period after the infection before the antibodies become detectable, and once incited, the antibodies stay for a long time, even after the pathogenic organisms have been eliminated.…”

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confidence: 99%

Diagnosis of Human Leptospirosis by Monoclonal Antibody-Based Antigen Detection in Urine

Saengjaruk¹,

Chaicumpa²,

Watt

et al. 2002

J Clin Microbiol

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Hybridomas secreting specific monoclonal antibodies (MAb) to all members of the genus Leptospira (clone LF9) and those that are specific only to the pathogenic species (clones LD5 and LE1) were produced. MAb LF9, which was immunoglobulin G1 (IgG1), reacted to a 38-kDa component of the sodium dodecyl sulfate-polyacrylamide gel electrophoresis-separated whole-cell lysates of all Leptospira spp., while MAb LD5 and MAb LE1, which were IgG1 and IgG2a, respectively, reacted to the 35-to 36-kDa components of all serogroups of the pathogenic species of Leptospira. The MAb LD5 was used in a dot blot-enzyme-linked immunosorbent assay (dot-ELISA) for detecting Leptospira antigen in urine samples serially collected from two groups of patients diagnosed with leptospirosis, i.e., 36 clinically diagnosed patients and 25 Leptospira culture confirmed patients. Their serum samples were tested serologically by IgM Dipstick assay, indirect immunofluorescence assay (IFA), and/or microscopic agglutination test (MAT). Urine samples of 26 patients diagnosed with other illnesses and 120 healthy individuals served as controls. For the first group of patients, who had been ill for an average of 3.4 days before hospitalization, the IgM Dipstick test, IFA, and MAT were positive for 69.4, 70.0, and 85.7% of patients, while the Leptospira antigenuria tested by the MAb-based dot-ELISA was positive for 75.0, 88.9, 97.2, 97.2, and 100% of patients on days 1, 2, 3, 7, and 14 of hospitalization, respectively. All but 1 of 11 patients whose serum samples collected on the first day of hospitalization were IgM seronegative, were positive by urine antigen test on day 1. This is strong evidence that detection of antigen in urine can provide diagnostic information that could be useful in directing early therapeutic intervention. The MAT was positive in 10 of 12 patients (83.3%) of the 25 culture-positive Leptospira patients who had been ill for an average of 5.04 days before hospitalization, and the Leptospira antigen was found in 64.0, 84.0, 96.0, 100, 100, 100, and 100% of the patients' urine samples collected on days 1, 2, 3, 4, 5, 6, and 7 of hospitalization, respectively. Leptospira antigenuria was found in 3 of the 26 patients diagnosed with other illnesses and 1 of the 120 healthy controls. The reasons for this positivity are discussed. The detection of antigen in urine by the monoclonal antibody-based dot-ELISA has high potential for rapid, sensitive, and specific diagnosis of leptospirosis at a low cost.

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“…Two studies have indicated high risk of bias in the patient selection in the applicability criteria 50,51 , and two studies demonstrated a high risk of bias in evaluating the index test 48,65 . In the other studies, there were some unclear applicability criteria in the index test and reference standard 19,21,[23][24][25][26]29,30,32,34,35,38,40,42,43,46,47,49,53,54,57,64,[67][68][69] . The robustness of the results was tested by repeating the analysis using a different statistical model (random effects model).…”

Section: Resultsmentioning

confidence: 99%

IgM ELISA for leptospirosis diagnosis: a systematic review and meta-analysis

Rosa

Reis

Simon

et al. 2017

Ciênc. saúde coletiva

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Immunodiagnosis of Human Leptospirosis by Dot-Elisa for the Detection of IgM, IgG, and IgA Antibodies

Cited by 29 publications

References 0 publications

Situación de la leptospirosis en el Urabá antioqueño colombiano: estudio seroepidemiológico y factores de riesgo en población general urbana

Situación de la leptospirosis en el Urabá antioqueño colombiano: estudio seroepidemiológico y factores de riesgo en población general urbana

Diagnosis of Human Leptospirosis by Monoclonal Antibody-Based Antigen Detection in Urine

IgM ELISA for leptospirosis diagnosis: a systematic review and meta-analysis

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