Immunodiagnosis of fasciolosis in horses and pigs using Western blots

Gorman, Thomas M.; Aballay, Jimena; Fredes, Fernando; Silva, Marco; Aguillón, Juan Carlos; Alcaíno, H

doi:10.1016/s0020-7519(97)00090-8

Cited by 18 publications

(13 citation statements)

References 5 publications

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“…Finally, several investigations noted the absence of information regarding fasciolosis in some animal species such as horses, rabbits and pigs, which in some countries may be important reservoir hosts of infection but are usually disregarded when treatment strategies are implemented (Apt et al 1993;Gorman et al 1997). The finding of a 60% seroprevalence hints that an elevated number of horses in the present research were exposed to F. hepatica metacercariae, and specific IgG antibodies were synthesized.…”

Section: Discussionmentioning

confidence: 61%

“…However, this method is not sensitive, and infections where the parasite burden is low or when the host is harbouring immature flukes in the liver parenchyma or the bile ducts may go undetected. Moreover, fluke egg elimination until 14-15 weeks occurred after the experimental infection of horses with F. hepatica metacercariae has been reported (Soulé et al 1989) and an intermittent egg output in these animals also (Gorman et al 1997). …”

Section: Introductionmentioning

confidence: 91%

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Enzyme-linked immunosorbent assays for the detection of equine antibodies specific to a recombinant Fasciola hepatica surface antigen in an endemic area

et al. 2011

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The utility of an enzyme-linked immunosorbent assay to determine the sensitization against the trematode Fasciola hepatica in horses from an endemic area (NW Spain) was assessed. Blood samples were collected from 536 horses and tested against a 2.9-kDa recombinant surface protein (FhrAPS) to estimate the presence of IgG antibodies. Data were analysed regarding several intrinsic (age, gender and breed) and extrinsic factors (aptitude and housing). The farm size (number of horses/farm) was also considered. Sixty percent (95% CI 56, 64) of the horses were positive to the FhrAPS-ELISA, with a significantly higher seroprevalence in the mares (67%). Foals reached the lowest percentage of sensitization against the trematode (12%), and a significant positive correlation between the seroprevalence of fasciolosis and the age of the horses was established. When considering all the factors together, the seroprevalence of fasciolosis was initially classified into two groups (nodes) regarding the age of the horses. The node composed of the horses older than 1 year was then divided into two other clusters according to their gender. The mares were finally classified and grouped into two nodes regarding their breed. We concluded that the FhrAPS-ELISA is very useful for the demonstration of specific equine IgG antibodies against F. hepatica. An elevated risk of exposition to this trematode in horses maintained in endemic areas was proven. The possible role of horses as reservoirs for F. hepatica infections is discussed.

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Section: Discussionmentioning

confidence: 61%

Section: Introductionmentioning

confidence: 91%

Enzyme-linked immunosorbent assays for the detection of equine antibodies specific to a recombinant Fasciola hepatica surface antigen in an endemic area

et al. 2011

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“…) and a blood ELISA which may have a high sensitivity but low specificity with detection of exposed in addition to infected horses (Gorman et al . ; Sol Arias et al . ; Acici et al .…”

Section: Discussionmentioning

confidence: 99%

“…Diagnosis of fluke in the horse is limited to a combination of history of environmental exposure, modified fluke egg sedimentation test (Boray test) which is reported to have a low sensitivity (Gorman et al . ; Acici et al . ) and elevation of gamma glutamyltransferase which intuitively should increase with biliary inflammation and damage.…”

Section: Discussionmentioning

confidence: 99%

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Severe eosinophilic cholangiohepatitis due to fluke infestation in a pony in Scotland

Raftery

Berman

Sutton

2015

Equine Veterinary Education

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Summary Fasciola hepatica infestation is reported as a cause of severe eosinophilic cholangiohepatitis in a 28‐year‐old Warmblood cross pony gelding. The gelding presented initially for investigation of acute laminitis found to be secondary to pars pituitary intermedia dysfunction (PPID). Six weeks of treatment including administration of pergolide achieved good control of the laminitis but there was a marked general clinical deterioration over this period with notable weight loss, tachycardia, anorexia and ventral oedema. Hepatobiliary enzymes were increased significantly and there was mild elevation of plasma bile acid concentration. An eosinophilia in both blood and peritoneal fluid specimens and histopathological confirmation of an eosinophilic cholangiohepatitis on examination of liver biopsies supported a primary parasitic aetiology. The pony was treated for Fasciola hepatica with triclabendazole 12 mg/kg bwt but clinical signs continued to progress. Due to poor clinical response the pony was subjected to euthanasia and post mortem examination revealed an overwhelming infestation of the biliary tracts with Fasciola hepatica and a secondary chronic and severe cholangiohepatitis with marked fibrosis. To the authors' knowledge this is the first equine report demonstrating the relationship between the clinical disease syndrome, eosinophilic cholangiohepatitis and hepatic fluke infestation. Depending on geographical location, fasciolosis should be considered as a differential diagnosis for equine hepatopathies and can result in severe disease.

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