Immunodetection of Lymphocyte Subpopulations Involved in Allograft Rejection in a Teleost,Dicentrarchus labrax(L.)

Immunoglobulin protein and gene transcripts in ovarian follicles throughout oogenesis in the teleost Dicentrarchus labrax

“…Adjacent sections were incubated for 18 h at room temperature with mAb DLT15 diluted 1:10 in 0.1 M PBS, pH 7.3, containing 5% normal horse serum, 5% normal sea bass serum and 0.1% sodium azide. mAb DLT15 (IgG 3 subclass isotype) was raised in mice against paraformaldehydefixed sea bass thymocytes and was established to be specific for thymocytes and peripheral T-cells (Scapigliati et al 1995(Scapigliati et al , 1999bAbelli et al 1999). Normal mouse serum or the myeloma culture medium substituted the primary antibody in negative control sections.…”

Kinetics of lymphocyte subpopulations in allogeneic grafted scales of ginbuna crucian carp

“…Abelli et al (1999) observed the infiltration of sIgM + cell into allografted tissue by immunohistochemical study. They discussed heterogeneity of the sIgM + cell populations involved in allograft rejection.…”

“…McKinney et al (1981) reported that in the holostean fish, Lepisosteus platyrhincus, the majority of infiltrated cells in acute allograft rejection were mononuclear leukocytes. Abelli et al (1999) showed that lymphocyte subpopulations were involved in allogeneic muscle graft rejection in sea bass, Dicentrarchus labrax (L.), using a monoclonal antibody (mAb) recognizing T cells. However, the subset of T cells involved in allograft rejection remains unknown.…”

Cellular activities during a mixed leucocyte reaction in the teleost sea bass Dicentrarchus labrax

“…This behaviour may be reproduced ''in vitro'' using the mixed leucocyte reaction (MLR), and ''in vivo'' leads to allograft rejection and to graft versus host reaction (GVRH). Both these ''in vivo'' activities have been shown to take place in teleost species such as damselfish [14], sea bass [15], and carp [16]. The ''in vitro'' activities of teleost T-cells have been known for many years, when peripheral blood leucocytes (PBL) from rainbow trout were found to exhibit an MLR against an allogeneic target [17].…”

“…However, despite the clear evidence of the involvement of T lymphocytes described in these previous works, their participation in fish MLR was only supposed, since no specific markers were available for direct analysis. We previously investigated the involvement of T-cells and B-cells during ''in vivo'' allograft rejection in sea bass by quantitative immunocytochemistry [15], and provided data on the participation of T lymphocytes during non-self recognition processes in fish.…”