Cellular activities during a mixed leucocyte reaction in the teleost sea bass Dicentrarchus labrax

Meloni, Sabrina; Zarletti, Gianpaolo; Benedetti, Stefania; Randelli, Elisa; Buonocore, Francesco; Scapigliati, Giuseppe

doi:10.1016/j.fsi.2005.10.001

Cited by 30 publications

(14 citation statements)

References 54 publications

(57 reference statements)

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“…MLC/MLR has been reported in several fish species upon in vitro incubation of allogeneic leukocytes (see Meloni et al, 2006). In channel catfish it has been reported that surface Ig-negative (sIg − ) lymphocytes were the responding cells in MLC (Miller et al,Fig.…”

Section: Discussionmentioning

confidence: 99%

Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish

Toda

Saito

Koike

et al. 2011

Developmental & Comparative Immunology

140

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Section: Discussionmentioning

confidence: 99%

Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish

Toda

Saito

Koike

et al. 2011

Developmental & Comparative Immunology

140

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“…Cell proliferation was determined by assaying intracellular ATP levels of leucocyte cultures using a luminometric assay (ATP-lite, Perkin Elmer) as previously described [26,27]. Briefly, 100 ml of culture medium containing 5 Â 10 5 leucocytes from control and VERv-treated fish were incubated for 48 h in L15 medium containing 5% FCS, antibiotics, and where required with 5 ml of the same VERv preparation obtained as above described, but inactivated by heat treatment (65 C, 20 min).…”

Section: Leucocytes and Cell Proliferationmentioning

confidence: 99%

“…1. Cell proliferation was measured by the quantitative determination of intracellular ATP, already shown to be related to cell viability and cell number in sea bass [27]. The bars show the content of intracellular ATP measured in cultures of leucocytes coming from samples inoculated with PBS (black bars), and from samples inoculated with VERv (grey bars).…”

Section: Cell Proliferationmentioning

confidence: 99%

Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus

Scapigliati

Buonocore

Randelli

et al. 2010

Fish & Shellfish Immunology

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Naïve sea bass juveniles (38.4 + or - 4.5 g) were intramuscularly infected with a sublethal dose of betanodavirus isolate 378/I03, followed after 43 days by a similar boosting. This infection resulted in an overall mortality of 7.6%. At various intervals, sampling of fish tissues was performed to investigate: i) B and T lymphocyte content in organs and tissues; ii), proliferation of leucocytes re-stimulated in vitro with inactivated virus; iii) presence of serum antibody specific for betanodavirus; iv) expression of genes coding for the following immunoregulatory molecules involved in innate and acquired responses: type I IFN, Mx, IL-1, Cox-2; IL-10, TGF-beta, TCRbeta, CD4, CD8alpha, IgM, by using a quantitative PCR array system developed for sea bass. The obtained results showed a detectable increase of T cells and B cells in PBL during betanodavirus infection. Furthermore, leucocytes obtained from blood, head kidney, and gills showed a detectable "in vitro" increase in viability upon addition of inactivated viral particles, as determined by measuring intracellular ATP concentration. ELISA analysis of sera showed that exposure to nodavirus induced a low, but specific antibody titer measured 43 days after infection, despite the presence of measurable levels of natural antibody. Finally, a strong upregulation of genes coding for type I IFN, Mx, and IgM was identified after both infection and boosting. Interestingly, an upregulation of Cox-2 until boosting, and of TGF-beta and IL-10 after boosting was also observed, while the other tested genes did not show any significant variations with respect to mock-treated fish. Overall, our work represents a first comprehensive analysis of cellular and molecular immune parameters in a fish species exposed to a pathogenic virus.

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“…In vitro, some of the alloreactive TCR þ channel catfish cytotoxic cell clones kill their targets [37], specific cell-mediated cytotoxicity (CMC) against allogeneic target cells has also been shown in rainbow trout [38], thus providing further evidence on the existence of cytotoxic T lymphocytes (CTL) in teleosts. In 2005, Meloni et al also confirmed the ''in vitro'' activities of T cells in teleost sea bass by mixed leukocyte reaction (MLR) and RT-PCR to analysis the expression of the T cell receptor (ß-subunit) mRNA [39]. Moreover, many evidences of ''in vivo'' activities of CTL have been shown in teleost species such as sea bass [40], ginbuna carp [41] and amago salmon (Oncorhynchus rhodurus) [42].…”

Section: Introductionmentioning

confidence: 96%

Molecular cloning and characterization of carp (Cyprinus carpio L.) CD8β and CD4-like genes

Sun

Shang

et al. 2007

Fish & Shellfish Immunology

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Cellular activities during a mixed leucocyte reaction in the teleost sea bass Dicentrarchus labrax

Cited by 30 publications

References 54 publications

Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish

Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish

Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus

Molecular cloning and characterization of carp (Cyprinus carpio L.) CD8β and CD4-like genes

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