Immunodetection of Herbicide 2,4-Dichlorophenoxyacetic Acid by Field-Effect Transistor-Based Biosensors

Хомутов, С. М.; Zherdev, Anatoly V.; Dzantiev, Boris B.; Reshetilov, A. N.

doi:10.1080/00032719408000306

Cited by 36 publications

(7 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The current requirements needed for the analysis of trace substances and pharmaceuticals in food industries and in clinical areas [59] have made of the immunosensors interesting alternatives that may provide fast, highly sensitive, specific, and automated analytical devices, continuous monitoring systems, etc. Although the most well known of these is the glucose sensor and other sensors employed for medical diagnoses, several immunosensors have also been developed for the detection of pesticides [60,61,62] and pollutants [63,64].…”

Section: Immunosensorsmentioning

confidence: 99%

Immunochemical determination of xenobiotics with endocrine disrupting effects

Estévez-Alberola

Marco

2004

Analytical and Bioanalytical Chemistry

View full text Add to dashboard Cite

This paper is a review with more than 100 references discussing the immunochemical methods reported in the literature for the most important man-made chemicals with suspected endocrine disrupting activity. Details regarding immunizing hapten design, antibody production, and the features (limit of detection, dynamic range, specificity) of the most important immunochemical methods developed (ELISA, FIIA, immunosorbents, immunosensors, etc.) are presented for important environmental pollutants such as bisphenol A, phthalates, alkylphenol polyethoxylates, alkylphenols, polychlorinated biphenyl compounds, and dioxins. Availability of commercial reagents and methods is reported.

show abstract

Section: Immunosensorsmentioning

confidence: 99%

Immunochemical determination of xenobiotics with endocrine disrupting effects

Estévez-Alberola

Marco

2004

Analytical and Bioanalytical Chemistry

View full text Add to dashboard Cite

show abstract

“…Several immunotechniques for its assay were described, involving ELISA [13 -17], ion-selective field effect transistor [18], potentiometric sensors [19] and piezoelectric sensors [12] as transducers. Amperometric sensors, which employed peroxidase as a label, have been developed in our laboratory [20,21].…”

Section: Introductionmentioning

confidence: 99%

The immunosensors for measurement of 2,4-dichlorophenoxyacetic acid based on electrochemical impedance spectroscopy

Navrátilová

Skládal

2004

Bioelectrochemistry

View full text Add to dashboard Cite

“…For comparison, the indirect competitive ELISA with immobilized 2,4-D-albumin conjugate and MAb E2/G2 in solution provided LOD of 0.2 pg/L, the total time of analysis being 2 h [24]; our previous immunosensor operating in a similar format as the above mentioned ELISA achieved LOD of 0.1 p g L in 1 h [20]. Another immunosensor based on a pH-sensitive ISFET and the 2,4-D, peroxidase tracer reports LOD of 1 pg/L [34].…”

Section: Optimization Of the Sensing System And Assay Conditionsmentioning

confidence: 98%

Disposable multichannel immunosensors for 2,4‐dichlorophenoxyacetic acid using acetylcholinesterase as an enzyme label

Kaláb

Skládal

1997

Electroanalysis

View full text Add to dashboard Cite

An improved version of the disposable multichannel immunochemical biosensor for the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) based on a screen-printed amperometric transducer and monoclonal antibodies (MAb) against 2,4-D is reported. Entrapment within a thin Nafion film was used for the direct immobilization of MAb at the electrode surface. The amount of the tracer (2,4-D conjugated to acetylcholinesterase) bound in a competitive immunochemical reaction was determined amperometrically using acetylthiocholine iodide as substrate. The measuring procedure (times of incubation with tracer and substrate, pH, tracer concentration) was optimized. The sensor was able to detect less than 0.01 pgL of free 2,4-D in water. One analysis (8 samples) was completed in 30 min (20min for immunochemical reaction, 5 min incubation with substrate, 5 min measurement). The performance of the immunosensor (two configurations) was evaluated on real samples (tap water) with added 2,4-D. The determined amounts (mean values 0.097 to 0.105 and 0.89 to 1.13) corresponded well with the added contents of 2,4-D (0.100 and 1.00 pgL, respectively).

show abstract

Immunodetection of Herbicide 2,4-Dichlorophenoxyacetic Acid by Field-Effect Transistor-Based Biosensors

Cited by 36 publications

References 5 publications

Immunochemical determination of xenobiotics with endocrine disrupting effects

Immunochemical determination of xenobiotics with endocrine disrupting effects

The immunosensors for measurement of 2,4-dichlorophenoxyacetic acid based on electrochemical impedance spectroscopy

Disposable multichannel immunosensors for 2,4‐dichlorophenoxyacetic acid using acetylcholinesterase as an enzyme label

Contact Info

Product

Resources

About